Lipid accumulation in lipomyces starkeyi MV-8 cultured in glucose media

Numerous problems in producing diesel have risen globally. Originally, plant oils and animals fats were used as biodiesel feedstock. However, this feedstock was incompatible due to the competition with food production supplies and large area needed. Hence, oleaginous yeast is looked as a further alt...

Full description

Bibliographic Details
Main Author:	Khairunnisa, Auma
Format:	Project Report
Language:	English English
Published:	Universiti Malaysia Sarawak, (UNIMAS) 2015
Subjects:	S Agriculture (General) SB Plant culture SF Animal culture
Online Access:	http://ir.unimas.my/10823/ http://ir.unimas.my/10823/1/Lipid%20Accumulation%20of%20Lipomyces%20Starkeyi%20MV-8%20Cultured%20in%20Glucose%20Media%20%2824%20pages%29.pdf http://ir.unimas.my/10823/2/Lipid%20Accumulation%20of%20Lipomyces%20Starkeyi%20MV-8%20Cultured%20in%20Glucose%20Media%20%28fulltext%29.pdf

id	unimas-10823
recordtype	eprints
spelling	unimas-108232016-03-07T07:38:04Z http://ir.unimas.my/10823/ Lipid accumulation in lipomyces starkeyi MV-8 cultured in glucose media Khairunnisa, Auma S Agriculture (General) SB Plant culture SF Animal culture Numerous problems in producing diesel have risen globally. Originally, plant oils and animals fats were used as biodiesel feedstock. However, this feedstock was incompatible due to the competition with food production supplies and large area needed. Hence, oleaginous yeast is looked as a further alternative as biodiesel feedstock. Lipomyces starkeyi is selected as it can accumulate higher lipid than other oleaginous yeasts. This study uses L. starkeyi MV-8 instead wild type strain which regularly produce lesser lipid. The study was performed to determine the lipid production of L. starkeyi MV-8 cultured in 2.5% (w/v) and 5.0% (w/v) concentration of glucose. L. starkeyi MV-8 was propagated for 8 days to determine the highest growth of biomass. Lipid accumulation of L. starkeyi MV-8 was performed at two different concentrations of glucose. Total carbohydrate test and lipid extraction were carried out to find out sugar consumption and lipid production respectively. The highest biomass of L. starkeyi MV-8 in 2.5% (w/v) and 5.0% (w/v) of glucose were obtained at hour 192 at respective 17.57 g/L and 20.10 g/L, while the highest lipid production of L. starkeyi MV-8 in 2.5% (w/v) and 5.0% (w/v) of glucose were 4.30 g/L and 6.00 g/L respectively. Based on these results, the percentages of lipid content in the L. starkeyi MV-8 biomass were 24.47% and 29.85% respectively for L. starkeyi MV-8 in 2.5% (w/v) and 5.0% (w/v) of glucose. In this study, L. starkeyi MV-8 cultured in 5.0% (w/v) of glucose concentration gave higher biomass and higher lipid than in 2.5% (w/v) of glucose concentration. Universiti Malaysia Sarawak, (UNIMAS) 2015 Project Report NonPeerReviewed text en http://ir.unimas.my/10823/1/Lipid%20Accumulation%20of%20Lipomyces%20Starkeyi%20MV-8%20Cultured%20in%20Glucose%20Media%20%2824%20pages%29.pdf text en http://ir.unimas.my/10823/2/Lipid%20Accumulation%20of%20Lipomyces%20Starkeyi%20MV-8%20Cultured%20in%20Glucose%20Media%20%28fulltext%29.pdf Khairunnisa, Auma (2015) Lipid accumulation in lipomyces starkeyi MV-8 cultured in glucose media. [Project Report] (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Sarawak
building	UNIMAS Institutional Repository
collection	Online Access
language	English English
topic	S Agriculture (General) SB Plant culture SF Animal culture
spellingShingle	S Agriculture (General) SB Plant culture SF Animal culture Khairunnisa, Auma Lipid accumulation in lipomyces starkeyi MV-8 cultured in glucose media
description	Numerous problems in producing diesel have risen globally. Originally, plant oils and animals fats were used as biodiesel feedstock. However, this feedstock was incompatible due to the competition with food production supplies and large area needed. Hence, oleaginous yeast is looked as a further alternative as biodiesel feedstock. Lipomyces starkeyi is selected as it can accumulate higher lipid than other oleaginous yeasts. This study uses L. starkeyi MV-8 instead wild type strain which regularly produce lesser lipid. The study was performed to determine the lipid production of L. starkeyi MV-8 cultured in 2.5% (w/v) and 5.0% (w/v) concentration of glucose. L. starkeyi MV-8 was propagated for 8 days to determine the highest growth of biomass. Lipid accumulation of L. starkeyi MV-8 was performed at two different concentrations of glucose. Total carbohydrate test and lipid extraction were carried out to find out sugar consumption and lipid production respectively. The highest biomass of L. starkeyi MV-8 in 2.5% (w/v) and 5.0% (w/v) of glucose were obtained at hour 192 at respective 17.57 g/L and 20.10 g/L, while the highest lipid production of L. starkeyi MV-8 in 2.5% (w/v) and 5.0% (w/v) of glucose were 4.30 g/L and 6.00 g/L respectively. Based on these results, the percentages of lipid content in the L. starkeyi MV-8 biomass were 24.47% and 29.85% respectively for L. starkeyi MV-8 in 2.5% (w/v) and 5.0% (w/v) of glucose. In this study, L. starkeyi MV-8 cultured in 5.0% (w/v) of glucose concentration gave higher biomass and higher lipid than in 2.5% (w/v) of glucose concentration.
format	Project Report
author	Khairunnisa, Auma
author_facet	Khairunnisa, Auma
author_sort	Khairunnisa, Auma
title	Lipid accumulation in lipomyces starkeyi MV-8 cultured in glucose media
title_short	Lipid accumulation in lipomyces starkeyi MV-8 cultured in glucose media
title_full	Lipid accumulation in lipomyces starkeyi MV-8 cultured in glucose media
title_fullStr	Lipid accumulation in lipomyces starkeyi MV-8 cultured in glucose media
title_full_unstemmed	Lipid accumulation in lipomyces starkeyi MV-8 cultured in glucose media
title_sort	lipid accumulation in lipomyces starkeyi mv-8 cultured in glucose media
publisher	Universiti Malaysia Sarawak, (UNIMAS)
publishDate	2015
url	http://ir.unimas.my/10823/ http://ir.unimas.my/10823/1/Lipid%20Accumulation%20of%20Lipomyces%20Starkeyi%20MV-8%20Cultured%20in%20Glucose%20Media%20%2824%20pages%29.pdf http://ir.unimas.my/10823/2/Lipid%20Accumulation%20of%20Lipomyces%20Starkeyi%20MV-8%20Cultured%20in%20Glucose%20Media%20%28fulltext%29.pdf
first_indexed	2018-09-06T15:49:33Z
last_indexed	2018-09-06T15:49:33Z
_version_	1610873803981717504

Lipid accumulation in lipomyces starkeyi MV-8 cultured in glucose media

Similar Items