Optimised conditions for PFGE separation of Pasteurella multocida B:2 DNA
Pulsed-field condition specific for Pasteurella multocida B:2 have been optimized to obtain a good separation resolution. Restriction enzyme, NotI, was the most suitable as it generates discernible number of bands. Other optimised electrophoretic conditions were: 1.5% agarose gel concentration, 200V...
| Main Authors: | , , , |
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| Format: | Article |
| Published: |
2001
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| Subjects: | |
| Online Access: | http://eprints.um.edu.my/9969/ http://eprints.um.edu.my/9969/1/Hasima_et_al.%2C_2001.pdf |