Protein-binding affinity of leucaena condensed tannins of differing molecular weights

Depending on their source, concentration, chemical structure, and molecular weight, condensed tannins (CTs) form insoluble complexes with protein, which could lead to ruminal bypass protein, benefiting animal production. In this study, CTs from Leuceana leucocephala hybrid were fractionated into fiv...

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Main Authors:	Huang, X.D., Liang, J.B., Tan, H.Y., Yahya, R., Long, R., Ho, Y.W.
Format:	Article
Published:	2011
Subjects:	QD Chemistry
Online Access:	http://eprints.um.edu.my/6747/

id	um-6747
recordtype	eprints
spelling	um-67472013-07-05T07:20:02Z Protein-binding affinity of leucaena condensed tannins of differing molecular weights Huang, X.D. Liang, J.B. Tan, H.Y. Yahya, R. Long, R. Ho, Y.W. QD Chemistry Depending on their source, concentration, chemical structure, and molecular weight, condensed tannins (CTs) form insoluble complexes with protein, which could lead to ruminal bypass protein, benefiting animal production. In this study, CTs from Leuceana leucocephala hybrid were fractionated into five fractions by a size exclusion chromatography procedure. The molecular weights of the CT fractions were determined using Q-TOF LC-MS, and the protein-binding affinities of the respective CT fractions were determined using a protein precipitation assay with bovine serum albumin (BSA) as the standard protein. The calculated number-average molecular weights (M n) were 1348.6, 857.1, 730.1, 726.0, and 497.1, and b values (the b value represents the CT quantity that is needed to bind half of the maximum precipitable BSA) of the different molecular weight fractions were 0.381, 0.510, 0.580, 0.636, and 0.780 for fractions 1, 2, 3, 4, and 5, respectively. The results indicated that, in general, CTs of higher molecular weight fractions have stronger protein-binding affinity than those of lower molecular weights. However, the number of hydroxyl units within the structure of CT polymers also affects the protein-binding affinity. 2011 Article PeerReviewed Huang, X.D.; Liang, J.B.; Tan, H.Y.; Yahya, R.; Long, R.; Ho, Y.W. (2011) Protein-binding affinity of leucaena condensed tannins of differing molecular weights. Journal of Agricultural and Food Chemistry <http://eprints.um.edu.my/view/publication/Journal_of_Agricultural_and_Food_Chemistry.html>, 59 (19). pp. 10677-10682. ISSN 00218561 http://eprints.um.edu.my/6747/
repository_type	Digital Repository
institution_category	Local University
institution	University Malaya
building	UM Research Repository
collection	Online Access
topic	QD Chemistry
spellingShingle	QD Chemistry Huang, X.D. Liang, J.B. Tan, H.Y. Yahya, R. Long, R. Ho, Y.W. Protein-binding affinity of leucaena condensed tannins of differing molecular weights
description	Depending on their source, concentration, chemical structure, and molecular weight, condensed tannins (CTs) form insoluble complexes with protein, which could lead to ruminal bypass protein, benefiting animal production. In this study, CTs from Leuceana leucocephala hybrid were fractionated into five fractions by a size exclusion chromatography procedure. The molecular weights of the CT fractions were determined using Q-TOF LC-MS, and the protein-binding affinities of the respective CT fractions were determined using a protein precipitation assay with bovine serum albumin (BSA) as the standard protein. The calculated number-average molecular weights (M n) were 1348.6, 857.1, 730.1, 726.0, and 497.1, and b values (the b value represents the CT quantity that is needed to bind half of the maximum precipitable BSA) of the different molecular weight fractions were 0.381, 0.510, 0.580, 0.636, and 0.780 for fractions 1, 2, 3, 4, and 5, respectively. The results indicated that, in general, CTs of higher molecular weight fractions have stronger protein-binding affinity than those of lower molecular weights. However, the number of hydroxyl units within the structure of CT polymers also affects the protein-binding affinity.
format	Article
author	Huang, X.D. Liang, J.B. Tan, H.Y. Yahya, R. Long, R. Ho, Y.W.
author_facet	Huang, X.D. Liang, J.B. Tan, H.Y. Yahya, R. Long, R. Ho, Y.W.
author_sort	Huang, X.D.
title	Protein-binding affinity of leucaena condensed tannins of differing molecular weights
title_short	Protein-binding affinity of leucaena condensed tannins of differing molecular weights
title_full	Protein-binding affinity of leucaena condensed tannins of differing molecular weights
title_fullStr	Protein-binding affinity of leucaena condensed tannins of differing molecular weights
title_full_unstemmed	Protein-binding affinity of leucaena condensed tannins of differing molecular weights
title_sort	protein-binding affinity of leucaena condensed tannins of differing molecular weights
publishDate	2011
url	http://eprints.um.edu.my/6747/
first_indexed	2018-09-06T05:20:58Z
last_indexed	2018-09-06T05:20:58Z
_version_	1610834256964091904

Protein-binding affinity of leucaena condensed tannins of differing molecular weights

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