Synthesized flavanoid-derived ligand reduced dengue virus type-2 replication in vitro

Synthesized flavanoid-derived ligand reduced dengue virus type-2 replication in vitro

Objective To investigate the antiviral property of a lead ligand, YK51 that was synthesized based on the flavanoid of a natural product toward dengue virus type-2 (DENV2) replication. Methods cRNA was isolated from HepG2 cells inoculated with 1 000 median tissue culture infective dose of DENV2 and t...

Full description

Bibliographic Details
Main Authors: Muhamad, M., Lee, Y.K., Rahman, N.A., Yusof, R.
Format: Article
Published: Elsevier 2017
Subjects:
Q Science (General)
R Medicine
Online Access:http://dx.doi.org/10.1016/j.apjtb.2016.10.013
http://dx.doi.org/10.1016/j.apjtb.2016.10.013
id um-19088
recordtype eprints
spelling um-190882018-09-04T04:56:50Z Synthesized flavanoid-derived ligand reduced dengue virus type-2 replication in vitro Muhamad, M. Lee, Y.K. Rahman, N.A. Yusof, R. Q Science (General) R Medicine Objective To investigate the antiviral property of a lead ligand, YK51 that was synthesized based on the flavanoid of a natural product toward dengue virus type-2 (DENV2) replication. Methods cRNA was isolated from HepG2 cells inoculated with 1 000 median tissue culture infective dose of DENV2 and treated with different doses of the ligand followed by RT-PCR to quantify the virus gene copies. Confocal microscopy of actin and tubulin redistribution was also performed. Results The quantitative RT-PCR result showed reduction of the DENV2 gene copies as the ligand concentration was increased. The confocal microscopy result showed increase in the tubulin intensity (79.6%) of infected BHK21 cells treated with the ligand, compared with the non-treated cells (54.8%). The 1.5-fold increase in the intensity of tubulin suggested that the ligand inhibitory effect stabilized the cellular microtubule structure. Conclusions The synthesized ligand YK51 reduced DENV2 viral load by inhibiting virus replication thus is highly potential to be developed as antiviral agent. Elsevier 2017 Article PeerReviewed http://dx.doi.org/10.1016/j.apjtb.2016.10.013 Muhamad, M.; Lee, Y.K.; Rahman, N.A.; Yusof, R. (2017) Synthesized flavanoid-derived ligand reduced dengue virus type-2 replication in vitro. Asian Pacific Journal of Tropical Biomedicine <http://eprints.um.edu.my/view/publication/Asian_Pacific_Journal_of_Tropical_Biomedicine.html>, 7 (2). pp. 91-95. ISSN 2221-1691 http://eprints.um.edu.my/19088/
repository_type Digital Repository
institution_category Local University
institution University Malaya
building UM Research Repository
collection Online Access
topic Q Science (General)
R Medicine
spellingShingle Q Science (General)
R Medicine
Muhamad, M.
Lee, Y.K.
Rahman, N.A.
Yusof, R.
Synthesized flavanoid-derived ligand reduced dengue virus type-2 replication in vitro
description Objective To investigate the antiviral property of a lead ligand, YK51 that was synthesized based on the flavanoid of a natural product toward dengue virus type-2 (DENV2) replication. Methods cRNA was isolated from HepG2 cells inoculated with 1 000 median tissue culture infective dose of DENV2 and treated with different doses of the ligand followed by RT-PCR to quantify the virus gene copies. Confocal microscopy of actin and tubulin redistribution was also performed. Results The quantitative RT-PCR result showed reduction of the DENV2 gene copies as the ligand concentration was increased. The confocal microscopy result showed increase in the tubulin intensity (79.6%) of infected BHK21 cells treated with the ligand, compared with the non-treated cells (54.8%). The 1.5-fold increase in the intensity of tubulin suggested that the ligand inhibitory effect stabilized the cellular microtubule structure. Conclusions The synthesized ligand YK51 reduced DENV2 viral load by inhibiting virus replication thus is highly potential to be developed as antiviral agent.
format Article
author Muhamad, M.
Lee, Y.K.
Rahman, N.A.
Yusof, R.
author_facet Muhamad, M.
Lee, Y.K.
Rahman, N.A.
Yusof, R.
author_sort Muhamad, M.
title Synthesized flavanoid-derived ligand reduced dengue virus type-2 replication in vitro
title_short Synthesized flavanoid-derived ligand reduced dengue virus type-2 replication in vitro
title_full Synthesized flavanoid-derived ligand reduced dengue virus type-2 replication in vitro
title_fullStr Synthesized flavanoid-derived ligand reduced dengue virus type-2 replication in vitro
title_full_unstemmed Synthesized flavanoid-derived ligand reduced dengue virus type-2 replication in vitro
title_sort synthesized flavanoid-derived ligand reduced dengue virus type-2 replication in vitro
publisher Elsevier
publishDate 2017
url http://dx.doi.org/10.1016/j.apjtb.2016.10.013
http://dx.doi.org/10.1016/j.apjtb.2016.10.013
first_indexed 2018-09-06T06:56:20Z
last_indexed 2018-09-06T06:56:20Z
_version_ 1610840270699495424

Similar Items