Association of nuclear and mitochondrial genes with audiological examinations in Iranian patients with nonaminoglycoside antibiotics-induced hearing loss

Mitochondrial DNA mutations play an important role in causing sensorineural hearing loss. The purpose of this study was to determine the association of the mitochondrial genes RNR1, MT-TL1, and ND1 as well as the nuclear genes GJB2 and GJB6 with audiological examinations in nonfamilial Iranians with...

Full description

Bibliographic Details
Main Authors:	Balali, M., Kamalidehghan, B., Farhadi, M., Ahmadipour, F., Ashkezari, M.D., Hemami, M.R., Arabzadeh, H., Falah, M., Meng, G.Y., Houshmand, M.
Format:	Article
Published:	Dove Medical Press 2016
Subjects:	Q Science (General) R Medicine
Online Access:	https://doi.org/10.2147/TCRM.S90581 https://doi.org/10.2147/TCRM.S90581

id	um-18454
recordtype	eprints
spelling	um-184542017-12-05T04:47:12Z Association of nuclear and mitochondrial genes with audiological examinations in Iranian patients with nonaminoglycoside antibiotics-induced hearing loss Balali, M. Kamalidehghan, B. Farhadi, M. Ahmadipour, F. Ashkezari, M.D. Hemami, M.R. Arabzadeh, H. Falah, M. Meng, G.Y. Houshmand, M. Q Science (General) R Medicine Mitochondrial DNA mutations play an important role in causing sensorineural hearing loss. The purpose of this study was to determine the association of the mitochondrial genes RNR1, MT-TL1, and ND1 as well as the nuclear genes GJB2 and GJB6 with audiological examinations in nonfamilial Iranians with cochlear implants, using polymerase chain reaction, DNA sequencing, and RNA secondary structure analysis. We found that there were no novel mutations in the mitochondrial gene 12S rRNA (MT-RNR1) in patients with and without GJB2 mutation (GJB2+and GJB2-, respectively), but a total of six polymorphisms were found. No mutations were observed in tRNALeu(UUR) (MT-TL1). Furthermore, eight polymorphisms were found in the mitochondrial ND1 gene. Additionally, no mutations were observed in the nuclear GJB6 gene in patients in the GJB2- and GJB2+ groups. The speech intelligibility rating and category of auditory perception tests were statistically assessed in patients in the GJB2- and GJB2+ groups. The results indicated that there was a significant difference (P <0.05) between the categories of auditory perception score in the GJB2- group compared to that in the GJB2+group. Successful cochlear implantation was observed among individuals with GJB2 mutations (GJB2+) and mitochondrial polymorphisms compared to those without GJB2 mutations (GJB2-). In conclusion, the outcome of this study suggests that variation in the mitochondrial and nuclear genes may influence the penetrance of deafness. Therefore, further genetic and functional studies are required to help patients in making the best choice for cochlear implants. Dove Medical Press 2016 Article PeerReviewed https://doi.org/10.2147/TCRM.S90581 Balali, M.; Kamalidehghan, B.; Farhadi, M.; Ahmadipour, F.; Ashkezari, M.D.; Hemami, M.R.; Arabzadeh, H.; Falah, M.; Meng, G.Y.; Houshmand, M. (2016) Association of nuclear and mitochondrial genes with audiological examinations in Iranian patients with nonaminoglycoside antibiotics-induced hearing loss. Therapeutics and Clinical Risk Management <http://eprints.um.edu.my/view/publication/Therapeutics_and_Clinical_Risk_Management.html>, 12. pp. 117-128. ISSN 1176-6336 http://eprints.um.edu.my/18454/
repository_type	Digital Repository
institution_category	Local University
institution	University Malaya
building	UM Research Repository
collection	Online Access
topic	Q Science (General) R Medicine
spellingShingle	Q Science (General) R Medicine Balali, M. Kamalidehghan, B. Farhadi, M. Ahmadipour, F. Ashkezari, M.D. Hemami, M.R. Arabzadeh, H. Falah, M. Meng, G.Y. Houshmand, M. Association of nuclear and mitochondrial genes with audiological examinations in Iranian patients with nonaminoglycoside antibiotics-induced hearing loss
description	Mitochondrial DNA mutations play an important role in causing sensorineural hearing loss. The purpose of this study was to determine the association of the mitochondrial genes RNR1, MT-TL1, and ND1 as well as the nuclear genes GJB2 and GJB6 with audiological examinations in nonfamilial Iranians with cochlear implants, using polymerase chain reaction, DNA sequencing, and RNA secondary structure analysis. We found that there were no novel mutations in the mitochondrial gene 12S rRNA (MT-RNR1) in patients with and without GJB2 mutation (GJB2+and GJB2-, respectively), but a total of six polymorphisms were found. No mutations were observed in tRNALeu(UUR) (MT-TL1). Furthermore, eight polymorphisms were found in the mitochondrial ND1 gene. Additionally, no mutations were observed in the nuclear GJB6 gene in patients in the GJB2- and GJB2+ groups. The speech intelligibility rating and category of auditory perception tests were statistically assessed in patients in the GJB2- and GJB2+ groups. The results indicated that there was a significant difference (P <0.05) between the categories of auditory perception score in the GJB2- group compared to that in the GJB2+group. Successful cochlear implantation was observed among individuals with GJB2 mutations (GJB2+) and mitochondrial polymorphisms compared to those without GJB2 mutations (GJB2-). In conclusion, the outcome of this study suggests that variation in the mitochondrial and nuclear genes may influence the penetrance of deafness. Therefore, further genetic and functional studies are required to help patients in making the best choice for cochlear implants.
format	Article
author	Balali, M. Kamalidehghan, B. Farhadi, M. Ahmadipour, F. Ashkezari, M.D. Hemami, M.R. Arabzadeh, H. Falah, M. Meng, G.Y. Houshmand, M.
author_facet	Balali, M. Kamalidehghan, B. Farhadi, M. Ahmadipour, F. Ashkezari, M.D. Hemami, M.R. Arabzadeh, H. Falah, M. Meng, G.Y. Houshmand, M.
author_sort	Balali, M.
title	Association of nuclear and mitochondrial genes with audiological examinations in Iranian patients with nonaminoglycoside antibiotics-induced hearing loss
title_short	Association of nuclear and mitochondrial genes with audiological examinations in Iranian patients with nonaminoglycoside antibiotics-induced hearing loss
title_full	Association of nuclear and mitochondrial genes with audiological examinations in Iranian patients with nonaminoglycoside antibiotics-induced hearing loss
title_fullStr	Association of nuclear and mitochondrial genes with audiological examinations in Iranian patients with nonaminoglycoside antibiotics-induced hearing loss
title_full_unstemmed	Association of nuclear and mitochondrial genes with audiological examinations in Iranian patients with nonaminoglycoside antibiotics-induced hearing loss
title_sort	association of nuclear and mitochondrial genes with audiological examinations in iranian patients with nonaminoglycoside antibiotics-induced hearing loss
publisher	Dove Medical Press
publishDate	2016
url	https://doi.org/10.2147/TCRM.S90581 https://doi.org/10.2147/TCRM.S90581
first_indexed	2018-09-06T06:51:07Z
last_indexed	2018-09-06T06:51:07Z
_version_	1610839928320557056

Association of nuclear and mitochondrial genes with audiological examinations in Iranian patients with nonaminoglycoside antibiotics-induced hearing loss

Similar Items