Comparative genome analysis of multiple vancomycin-resistant Enterococcus faecium isolated from two fatal cases

Enterococcus faecium is both a commensal of the human intestinal tract and an opportunistic pathogen. The increasing incidence of enterococcal infections is mainly due to the ability of this organism to develop resistance to multiple antibiotics, including vancomycin. The aim of this study was to pe...

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Main Authors:	Lim, S.Y., Yap, K.P., Teh, C.S.J., Jabar, K.A., Thong, K.L.
Format:	Article
Published:	Elsevier 2017
Subjects:	Q Science (General) R Medicine
Online Access:	https://doi.org/10.1016/j.meegid.2016.12.029 https://doi.org/10.1016/j.meegid.2016.12.029 http://eprints.um.edu.my/18384/1/Comparative_genome_analysis_of_multiple_vancomycin%2Dresistant_Enterococcus.pdf

id	um-18384
recordtype	eprints
spelling	um-183842017-11-30T02:10:57Z Comparative genome analysis of multiple vancomycin-resistant Enterococcus faecium isolated from two fatal cases Lim, S.Y. Yap, K.P. Teh, C.S.J. Jabar, K.A. Thong, K.L. Q Science (General) R Medicine Enterococcus faecium is both a commensal of the human intestinal tract and an opportunistic pathogen. The increasing incidence of enterococcal infections is mainly due to the ability of this organism to develop resistance to multiple antibiotics, including vancomycin. The aim of this study was to perform comparative genome analyses on four vancomycin-resistant Enterococcus faecium (VREfm) strains isolated from two fatal cases in a tertiary hospital in Malaysia. Two sequence types, ST80 and ST203, were identified which belong to the clinically important clonal complex (CC) 17. This is the first report on the emergence of ST80 strains in Malaysia. Three of the studied strains (VREr5, VREr6, VREr7) were each isolated from different body sites of a single patient (patient Y) and had different PFGE patterns. While VREr6 and VREr7 were phenotypically and genotypically similar, the initial isolate, VREr5, was found to be more similar to VRE2 isolated from another patient (patient X), in terms of the genome contents, sequence types and phylogenomic relationship. Both the clinical records and genome sequence data suggested that patient Y was infected by multiple strains from different clones and the strain that infected patient Y could have derived from the same clone from patient X. These multidrug resistant strains harbored a number of virulence genes such as the epa locus and pilus-associated genes which could enhance their persistence. Apart from that, a homolog of E. faecalis bee locus was identified in VREr5 which might be involved in biofilm formation. Overall, our comparative genomic analyses had provided insight into the genetic relatedness, as well as the virulence potential, of the four clinical strains. Elsevier 2017-12-25 Article PeerReviewed application/pdf http://eprints.um.edu.my/18384/1/Comparative_genome_analysis_of_multiple_vancomycin%2Dresistant_Enterococcus.pdf https://doi.org/10.1016/j.meegid.2016.12.029 Lim, S.Y.; Yap, K.P.; Teh, C.S.J.; Jabar, K.A.; Thong, K.L. (2017) Comparative genome analysis of multiple vancomycin-resistant Enterococcus faecium isolated from two fatal cases. Infection, Genetics and Evolution <http://eprints.um.edu.my/view/publication/Infection,_Genetics_and_Evolution.html>, 49. pp. 55-65. ISSN 1567-1348 http://eprints.um.edu.my/18384/
repository_type	Digital Repository
institution_category	Local University
institution	University Malaya
building	UM Research Repository
collection	Online Access
topic	Q Science (General) R Medicine
spellingShingle	Q Science (General) R Medicine Lim, S.Y. Yap, K.P. Teh, C.S.J. Jabar, K.A. Thong, K.L. Comparative genome analysis of multiple vancomycin-resistant Enterococcus faecium isolated from two fatal cases
description	Enterococcus faecium is both a commensal of the human intestinal tract and an opportunistic pathogen. The increasing incidence of enterococcal infections is mainly due to the ability of this organism to develop resistance to multiple antibiotics, including vancomycin. The aim of this study was to perform comparative genome analyses on four vancomycin-resistant Enterococcus faecium (VREfm) strains isolated from two fatal cases in a tertiary hospital in Malaysia. Two sequence types, ST80 and ST203, were identified which belong to the clinically important clonal complex (CC) 17. This is the first report on the emergence of ST80 strains in Malaysia. Three of the studied strains (VREr5, VREr6, VREr7) were each isolated from different body sites of a single patient (patient Y) and had different PFGE patterns. While VREr6 and VREr7 were phenotypically and genotypically similar, the initial isolate, VREr5, was found to be more similar to VRE2 isolated from another patient (patient X), in terms of the genome contents, sequence types and phylogenomic relationship. Both the clinical records and genome sequence data suggested that patient Y was infected by multiple strains from different clones and the strain that infected patient Y could have derived from the same clone from patient X. These multidrug resistant strains harbored a number of virulence genes such as the epa locus and pilus-associated genes which could enhance their persistence. Apart from that, a homolog of E. faecalis bee locus was identified in VREr5 which might be involved in biofilm formation. Overall, our comparative genomic analyses had provided insight into the genetic relatedness, as well as the virulence potential, of the four clinical strains.
format	Article
author	Lim, S.Y. Yap, K.P. Teh, C.S.J. Jabar, K.A. Thong, K.L.
author_facet	Lim, S.Y. Yap, K.P. Teh, C.S.J. Jabar, K.A. Thong, K.L.
author_sort	Lim, S.Y.
title	Comparative genome analysis of multiple vancomycin-resistant Enterococcus faecium isolated from two fatal cases
title_short	Comparative genome analysis of multiple vancomycin-resistant Enterococcus faecium isolated from two fatal cases
title_full	Comparative genome analysis of multiple vancomycin-resistant Enterococcus faecium isolated from two fatal cases
title_fullStr	Comparative genome analysis of multiple vancomycin-resistant Enterococcus faecium isolated from two fatal cases
title_full_unstemmed	Comparative genome analysis of multiple vancomycin-resistant Enterococcus faecium isolated from two fatal cases
title_sort	comparative genome analysis of multiple vancomycin-resistant enterococcus faecium isolated from two fatal cases
publisher	Elsevier
publishDate	2017
url	https://doi.org/10.1016/j.meegid.2016.12.029 https://doi.org/10.1016/j.meegid.2016.12.029 http://eprints.um.edu.my/18384/1/Comparative_genome_analysis_of_multiple_vancomycin%2Dresistant_Enterococcus.pdf
first_indexed	2018-09-06T06:50:27Z
last_indexed	2018-09-06T06:50:27Z
_version_	1610839886983593984

Comparative genome analysis of multiple vancomycin-resistant Enterococcus faecium isolated from two fatal cases

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