Ellagic acid increases osteocalcin and alkaline phosphatase after tooth extraction in nicotinic-treated rats

Objectives:-To examine the effect of nicotine (Ni) on bone socket healing treated with Ellagic acid (EA) after tooth extraction in rat. Materials and Methods: Thirty-Two Sprague Dawley (SD) male rats were divided into four groups. The group 1 was administrated with distilled water intragastrically a...

Full description

Bibliographic Details
Main Authors:	Al-Obaidi, M.M.J., Al-Bayaty, F.H., Batran, R.A., Ibrahim, O.E., Daher, A.M.
Format:	Article
Published:	Bentham Science Publishers 2016
Subjects:	R Medicine RK Dentistry
Online Access:	https://doi.org/10.2174/138161282216160428002842 https://doi.org/10.2174/138161282216160428002842

id	um-18255
recordtype	eprints
spelling	um-182552017-11-14T04:13:05Z Ellagic acid increases osteocalcin and alkaline phosphatase after tooth extraction in nicotinic-treated rats Al-Obaidi, M.M.J. Al-Bayaty, F.H. Batran, R.A. Ibrahim, O.E. Daher, A.M. R Medicine RK Dentistry Objectives:-To examine the effect of nicotine (Ni) on bone socket healing treated with Ellagic acid (EA) after tooth extraction in rat. Materials and Methods: Thirty-Two Sprague Dawley (SD) male rats were divided into four groups. The group 1 was administrated with distilled water intragastrically and injected sterile saline subcutaneously. The group 2 was administrated with EA orally and injected with sterile saline subcutaneously. The groups 3 & 4 were subcutaneously exposed to Ni for 4 weeks twice daily before tooth extraction procedure, and maintained Ni injection until the animals were sacrificed. After one month Ni exposure, the group 4 was fed with EA while continuing Ni injection. All the groups were anesthetized, and the upper left incisor was extracted. Four rats from each group were sacrificed on 14th and 28th days. Tumour necrosis factor alpha (TNFα), Interleukin-1 beta (IL-1β) and Interleukin-6 (IL-6) were applied to assess in serum rat at 14th and 28th days. Superoxide dismutase (SOD) and Thiobarbituric acid reactive substances (TBRAS) levels were assessed to evaluate the antioxidant status and lipid peroxidation accordingly after tooth extraction in homogenized gingival maxilla tissue of rat at 14th and 28th days. The socket hard tissue was stained by eosin and hematoxylin (H&E); immunohistochemical technique was used to assess the healing process by Osteocalcin (OCN) and Alkaline Phosphatase (ALP) biomarkers. Results: Ni-induced rats administered with EA compound (Group 4) dropped the elevated concentration of pro-inflammatory cytokines significantly when compared to Ni-induced rats (Group 3) (p<0.05). Ni-induced rats administrated with EA compound (Group 4) showed significant production of SOD and recession in TBRAS level when compared to Ni-induced rats (Group 3) (p<0.05). The immunohistochemistry analysis has revealed that OCN and ALP have presented stronger expression in Ni-induced rats treated with EA (Group 4), as against Ni-induced rats (Group 3). Conclusion: We have concluded that, Ni-induced rats, treated with EA have exerted positive effect on the trabecular bone formation after tooth extraction in nicotinic rats could be due to the antioxidant activity of EA which lead to upregulate of OCN and ALP proteins which are responsible for osteogenesis. Bentham Science Publishers 2016 Article PeerReviewed https://doi.org/10.2174/138161282216160428002842 Al-Obaidi, M.M.J.; Al-Bayaty, F.H.; Batran, R.A.; Ibrahim, O.E.; Daher, A.M. (2016) Ellagic acid increases osteocalcin and alkaline phosphatase after tooth extraction in nicotinic-treated rats. Current Pharmaceutical Design <http://eprints.um.edu.my/view/publication/Current_Pharmaceutical_Design.html>, 22 (16). pp. 2403-2410. ISSN 1381-6128 http://eprints.um.edu.my/18255/
repository_type	Digital Repository
institution_category	Local University
institution	University Malaya
building	UM Research Repository
collection	Online Access
topic	R Medicine RK Dentistry
spellingShingle	R Medicine RK Dentistry Al-Obaidi, M.M.J. Al-Bayaty, F.H. Batran, R.A. Ibrahim, O.E. Daher, A.M. Ellagic acid increases osteocalcin and alkaline phosphatase after tooth extraction in nicotinic-treated rats
description	Objectives:-To examine the effect of nicotine (Ni) on bone socket healing treated with Ellagic acid (EA) after tooth extraction in rat. Materials and Methods: Thirty-Two Sprague Dawley (SD) male rats were divided into four groups. The group 1 was administrated with distilled water intragastrically and injected sterile saline subcutaneously. The group 2 was administrated with EA orally and injected with sterile saline subcutaneously. The groups 3 & 4 were subcutaneously exposed to Ni for 4 weeks twice daily before tooth extraction procedure, and maintained Ni injection until the animals were sacrificed. After one month Ni exposure, the group 4 was fed with EA while continuing Ni injection. All the groups were anesthetized, and the upper left incisor was extracted. Four rats from each group were sacrificed on 14th and 28th days. Tumour necrosis factor alpha (TNFα), Interleukin-1 beta (IL-1β) and Interleukin-6 (IL-6) were applied to assess in serum rat at 14th and 28th days. Superoxide dismutase (SOD) and Thiobarbituric acid reactive substances (TBRAS) levels were assessed to evaluate the antioxidant status and lipid peroxidation accordingly after tooth extraction in homogenized gingival maxilla tissue of rat at 14th and 28th days. The socket hard tissue was stained by eosin and hematoxylin (H&E); immunohistochemical technique was used to assess the healing process by Osteocalcin (OCN) and Alkaline Phosphatase (ALP) biomarkers. Results: Ni-induced rats administered with EA compound (Group 4) dropped the elevated concentration of pro-inflammatory cytokines significantly when compared to Ni-induced rats (Group 3) (p<0.05). Ni-induced rats administrated with EA compound (Group 4) showed significant production of SOD and recession in TBRAS level when compared to Ni-induced rats (Group 3) (p<0.05). The immunohistochemistry analysis has revealed that OCN and ALP have presented stronger expression in Ni-induced rats treated with EA (Group 4), as against Ni-induced rats (Group 3). Conclusion: We have concluded that, Ni-induced rats, treated with EA have exerted positive effect on the trabecular bone formation after tooth extraction in nicotinic rats could be due to the antioxidant activity of EA which lead to upregulate of OCN and ALP proteins which are responsible for osteogenesis.
format	Article
author	Al-Obaidi, M.M.J. Al-Bayaty, F.H. Batran, R.A. Ibrahim, O.E. Daher, A.M.
author_facet	Al-Obaidi, M.M.J. Al-Bayaty, F.H. Batran, R.A. Ibrahim, O.E. Daher, A.M.
author_sort	Al-Obaidi, M.M.J.
title	Ellagic acid increases osteocalcin and alkaline phosphatase after tooth extraction in nicotinic-treated rats
title_short	Ellagic acid increases osteocalcin and alkaline phosphatase after tooth extraction in nicotinic-treated rats
title_full	Ellagic acid increases osteocalcin and alkaline phosphatase after tooth extraction in nicotinic-treated rats
title_fullStr	Ellagic acid increases osteocalcin and alkaline phosphatase after tooth extraction in nicotinic-treated rats
title_full_unstemmed	Ellagic acid increases osteocalcin and alkaline phosphatase after tooth extraction in nicotinic-treated rats
title_sort	ellagic acid increases osteocalcin and alkaline phosphatase after tooth extraction in nicotinic-treated rats
publisher	Bentham Science Publishers
publishDate	2016
url	https://doi.org/10.2174/138161282216160428002842 https://doi.org/10.2174/138161282216160428002842
first_indexed	2018-09-06T06:49:20Z
last_indexed	2018-09-06T06:49:20Z
_version_	1610839816024358912

Ellagic acid increases osteocalcin and alkaline phosphatase after tooth extraction in nicotinic-treated rats

Similar Items