Influence of nicotine on the adherence of Candida albicans ATCC 14053 and Candida parapsilosis ATCC 22019 and expression of selected binding-related genes

Smoking is considered one of the main factors for development of dental plaque. Yeasts have been described to contribute to the plaque community. The aim of this study was to investigate the influence of nicotine on the growth of planktonic cells and biofilm, cell aggregation, surface hydrophobicity...

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Main Authors:	Gunasegar, S., Himratul-Aznita, W.H.
Format:	Article
Published:	Taylor & Francis 2017
Subjects:	Practice of dentistry. Dental economics
Online Access:	http://www.tandfonline.com/doi/full/10.1080/13102818.2017.1334593 http://www.tandfonline.com/doi/full/10.1080/13102818.2017.1334593 http://eprints.um.edu.my/17791/1/Influence_of_nicotine_on_the_adherence_of_Candida_albicans_ATCC_14053_and_Candida_parapsilosis_ATCC_22019_and_expression_of_selected_binding_related.pdf

id	um-17791
recordtype	eprints
spelling	um-177912017-09-13T01:55:37Z Influence of nicotine on the adherence of Candida albicans ATCC 14053 and Candida parapsilosis ATCC 22019 and expression of selected binding-related genes Gunasegar, S. Himratul-Aznita, W.H. Practice of dentistry. Dental economics Smoking is considered one of the main factors for development of dental plaque. Yeasts have been described to contribute to the plaque community. The aim of this study was to investigate the influence of nicotine on the growth of planktonic cells and biofilm, cell aggregation, surface hydrophobicity, cell adherence and binding-related genes expressed by Candida albicans and Candida parapsilosis in vitro. The relative number of viable fungal cells was determined based on viable plate counts. The biofilm growth was quantified using the crystal violet assay. Reverse transcription-polymerase chain reaction was used to evaluate the regulation of selected genes associated with adherence. The results indicated that nicotine enhanced the growth of both planktonic and biofilm oral fungal cells. Cell surface hydrophobicity and the expression of Hyphal Wall Protein 1 (HWP1) and agglutinin-like sequences 3 (ALS3) of C. albicans and C. parapsilosis were found to increase relative to the nicotine concentrations. The results suggest that nicotine can enhance the growth of C. albicans and C. parapsilosis in vitro and influence their adherence to the surface of microplate wells that mimic as the tooth surface. Taylor & Francis 2017 Article PeerReviewed application/pdf http://eprints.um.edu.my/17791/1/Influence_of_nicotine_on_the_adherence_of_Candida_albicans_ATCC_14053_and_Candida_parapsilosis_ATCC_22019_and_expression_of_selected_binding_related.pdf http://www.tandfonline.com/doi/full/10.1080/13102818.2017.1334593 Gunasegar, S.; Himratul-Aznita, W.H. (2017) Influence of nicotine on the adherence of Candida albicans ATCC 14053 and Candida parapsilosis ATCC 22019 and expression of selected binding-related genes. Biotechnology & Biotechnological Equipment <http://eprints.um.edu.my/view/publication/Biotechnology_=26_Biotechnological_Equipment.html>, 31 (4). pp. 807-814. ISSN 1310-2818 http://eprints.um.edu.my/17791/
repository_type	Digital Repository
institution_category	Local University
institution	University Malaya
building	UM Research Repository
collection	Online Access
topic	Practice of dentistry. Dental economics
spellingShingle	Practice of dentistry. Dental economics Gunasegar, S. Himratul-Aznita, W.H. Influence of nicotine on the adherence of Candida albicans ATCC 14053 and Candida parapsilosis ATCC 22019 and expression of selected binding-related genes
description	Smoking is considered one of the main factors for development of dental plaque. Yeasts have been described to contribute to the plaque community. The aim of this study was to investigate the influence of nicotine on the growth of planktonic cells and biofilm, cell aggregation, surface hydrophobicity, cell adherence and binding-related genes expressed by Candida albicans and Candida parapsilosis in vitro. The relative number of viable fungal cells was determined based on viable plate counts. The biofilm growth was quantified using the crystal violet assay. Reverse transcription-polymerase chain reaction was used to evaluate the regulation of selected genes associated with adherence. The results indicated that nicotine enhanced the growth of both planktonic and biofilm oral fungal cells. Cell surface hydrophobicity and the expression of Hyphal Wall Protein 1 (HWP1) and agglutinin-like sequences 3 (ALS3) of C. albicans and C. parapsilosis were found to increase relative to the nicotine concentrations. The results suggest that nicotine can enhance the growth of C. albicans and C. parapsilosis in vitro and influence their adherence to the surface of microplate wells that mimic as the tooth surface.
format	Article
author	Gunasegar, S. Himratul-Aznita, W.H.
author_facet	Gunasegar, S. Himratul-Aznita, W.H.
author_sort	Gunasegar, S.
title	Influence of nicotine on the adherence of Candida albicans ATCC 14053 and Candida parapsilosis ATCC 22019 and expression of selected binding-related genes
title_short	Influence of nicotine on the adherence of Candida albicans ATCC 14053 and Candida parapsilosis ATCC 22019 and expression of selected binding-related genes
title_full	Influence of nicotine on the adherence of Candida albicans ATCC 14053 and Candida parapsilosis ATCC 22019 and expression of selected binding-related genes
title_fullStr	Influence of nicotine on the adherence of Candida albicans ATCC 14053 and Candida parapsilosis ATCC 22019 and expression of selected binding-related genes
title_full_unstemmed	Influence of nicotine on the adherence of Candida albicans ATCC 14053 and Candida parapsilosis ATCC 22019 and expression of selected binding-related genes
title_sort	influence of nicotine on the adherence of candida albicans atcc 14053 and candida parapsilosis atcc 22019 and expression of selected binding-related genes
publisher	Taylor & Francis
publishDate	2017
url	http://www.tandfonline.com/doi/full/10.1080/13102818.2017.1334593 http://www.tandfonline.com/doi/full/10.1080/13102818.2017.1334593 http://eprints.um.edu.my/17791/1/Influence_of_nicotine_on_the_adherence_of_Candida_albicans_ATCC_14053_and_Candida_parapsilosis_ATCC_22019_and_expression_of_selected_binding_related.pdf
first_indexed	2018-09-06T06:45:04Z
last_indexed	2018-09-06T06:45:04Z
_version_	1610839547995750400

Influence of nicotine on the adherence of Candida albicans ATCC 14053 and Candida parapsilosis ATCC 22019 and expression of selected binding-related genes

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