Inhibition of dengue virus entry into target cells using synthetic antiviral peptides

Despite the importance of DENV as a human pathogen, there is no specific treatment or protective vaccine. Successful entry into the host cells is necessary for establishing the infection. Recently, the virus entry step has become an attractive therapeutic strategy because it represents a barrier to...

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Main Authors:	Alhoot, M.A., Rathinam, A.K., Wang, S.M., Manikam, R., Sekaran, S.D.
Format:	Article
Published:	2013
Subjects:	R Medicine
Online Access:	http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3638295/ http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3638295/

id	um-10629
recordtype	eprints
spelling	um-106292014-11-05T00:55:50Z Inhibition of dengue virus entry into target cells using synthetic antiviral peptides Alhoot, M.A. Rathinam, A.K. Wang, S.M. Manikam, R. Sekaran, S.D. R Medicine Despite the importance of DENV as a human pathogen, there is no specific treatment or protective vaccine. Successful entry into the host cells is necessary for establishing the infection. Recently, the virus entry step has become an attractive therapeutic strategy because it represents a barrier to suppress the onset of the infection. Four putative antiviral peptides were designed to target domain III of DENV-2 E protein using BioMoDroid algorithm. Two peptides showed significant inhibition of DENV when simultaneously incubated as shown by plaque formation assay, RT-qPCR, and Western blot analysis. Both DET4 and DET2 showed significant inhibition of virus entry (84.6 and 40.6 respectively) using micromolar concentrations. Furthermore, the TEM images showed that the inhibitory peptides caused structural abnormalities and alteration of the arrangement of the viral E protein, which interferes with virus binding and entry. Inhibition of DENV entry during the initial stages of infection can potentially reduce the viremia in infected humans resulting in prevention of the progression of dengue fever to the severe life-threatening infection, reduce the infected vector numbers, and thus break the transmission cycle. Moreover these peptides though designed against the conserved region in DENV-2 would have the potential to be active against all the serotypes of dengue and might be considered as Hits to begin designing and developing of more potent analogous peptides that could constitute as promising therapeutic agents for attenuating dengue infection. 2013 Article PeerReviewed http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3638295/ Alhoot, M.A.; Rathinam, A.K.; Wang, S.M.; Manikam, R.; Sekaran, S.D. (2013) Inhibition of dengue virus entry into target cells using synthetic antiviral peptides. International Journal of Medical Sciences <http://eprints.um.edu.my/view/publication/International_Journal_of_Medical_Sciences.html>, 10 (6). pp. 719-729. ISSN 1449-1907 http://eprints.um.edu.my/10629/
repository_type	Digital Repository
institution_category	Local University
institution	University Malaya
building	UM Research Repository
collection	Online Access
topic	R Medicine
spellingShingle	R Medicine Alhoot, M.A. Rathinam, A.K. Wang, S.M. Manikam, R. Sekaran, S.D. Inhibition of dengue virus entry into target cells using synthetic antiviral peptides
description	Despite the importance of DENV as a human pathogen, there is no specific treatment or protective vaccine. Successful entry into the host cells is necessary for establishing the infection. Recently, the virus entry step has become an attractive therapeutic strategy because it represents a barrier to suppress the onset of the infection. Four putative antiviral peptides were designed to target domain III of DENV-2 E protein using BioMoDroid algorithm. Two peptides showed significant inhibition of DENV when simultaneously incubated as shown by plaque formation assay, RT-qPCR, and Western blot analysis. Both DET4 and DET2 showed significant inhibition of virus entry (84.6 and 40.6 respectively) using micromolar concentrations. Furthermore, the TEM images showed that the inhibitory peptides caused structural abnormalities and alteration of the arrangement of the viral E protein, which interferes with virus binding and entry. Inhibition of DENV entry during the initial stages of infection can potentially reduce the viremia in infected humans resulting in prevention of the progression of dengue fever to the severe life-threatening infection, reduce the infected vector numbers, and thus break the transmission cycle. Moreover these peptides though designed against the conserved region in DENV-2 would have the potential to be active against all the serotypes of dengue and might be considered as Hits to begin designing and developing of more potent analogous peptides that could constitute as promising therapeutic agents for attenuating dengue infection.
format	Article
author	Alhoot, M.A. Rathinam, A.K. Wang, S.M. Manikam, R. Sekaran, S.D.
author_facet	Alhoot, M.A. Rathinam, A.K. Wang, S.M. Manikam, R. Sekaran, S.D.
author_sort	Alhoot, M.A.
title	Inhibition of dengue virus entry into target cells using synthetic antiviral peptides
title_short	Inhibition of dengue virus entry into target cells using synthetic antiviral peptides
title_full	Inhibition of dengue virus entry into target cells using synthetic antiviral peptides
title_fullStr	Inhibition of dengue virus entry into target cells using synthetic antiviral peptides
title_full_unstemmed	Inhibition of dengue virus entry into target cells using synthetic antiviral peptides
title_sort	inhibition of dengue virus entry into target cells using synthetic antiviral peptides
publishDate	2013
url	http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3638295/ http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3638295/
first_indexed	2018-09-06T05:51:53Z
last_indexed	2018-09-06T05:51:53Z
_version_	1610836201705570304

Inhibition of dengue virus entry into target cells using synthetic antiviral peptides

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