Phenotypic detection of Metallo-β-Lactamase in Imipenem resistant Pseudomonas aeruginosa
Carbapenems are the primary choice of treatment for severe Pseudomonas aeruginosa infection. However, the emergence of carbapenem resistance due to the production of metallo β-lactamases (MBLs) is of global concern. In this study, 90 imipenem-(IPM- or IP-) resistant P. aeruginosa (IRPA) isolates, i...
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| Online Access: | http://eprints.um.edu.my/10498/ http://eprints.um.edu.my/10498/1/Phenotypic_Detection_of_Metallo%2D%CE%B2%2DLactamase_in_Imipenem%2DResistant_Pseudomonas_aeruginosa.pdf |
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um-104982014-10-14T01:22:37Z Phenotypic detection of Metallo-β-Lactamase in Imipenem resistant Pseudomonas aeruginosa Khosravi, Y. Loke, M.F. Tay, S.T. Vadivelu, J. Q Science (General) R Medicine (General) Carbapenems are the primary choice of treatment for severe Pseudomonas aeruginosa infection. However, the emergence of carbapenem resistance due to the production of metallo β-lactamases (MBLs) is of global concern. In this study, 90 imipenem-(IPM- or IP-) resistant P. aeruginosa (IRPA) isolates, including 32 previously tested positive and genotyped for MBL genes by PCR, were subjected to double-disk synergy test (DDST), combined disk test (CDT), and imipenem/imipenem-inhibitor (IP/IPI) E-test to evaluate their MBLs detection capability. All three methods were shown to have a sensitivity of 100%. However, DDST was the most specific of the three (96.6%), followed by IP/IPI E-test interpreted based on the single criteria of IP/IPI ≥ 8 as positive (62.1%), and CDT was the least specific (43.1%). Based on the data from this evaluation, we propose that only IRPA with IP MIC > 16 μg/mL and IP/IPI ≥ 8 by IP/IPI E-test should be taken as positive for MBL activity.With the new dual interpretation criteria,the MBL IP/IPI E-test was shown to achieve 100% sensitivity as well as specificity for the IRPA in this study. Therefore, the IP/IPI E-test is a viable alternative phenotypic assay to detect MBL production in IRPA in our population in circumstances where PCR detection is not a feasible option. 2012 Article PeerReviewed application/pdf http://eprints.um.edu.my/10498/1/Phenotypic_Detection_of_Metallo%2D%CE%B2%2DLactamase_in_Imipenem%2DResistant_Pseudomonas_aeruginosa.pdf Khosravi, Y.; Loke, M.F.; Tay, S.T.; Vadivelu, J. (2012) Phenotypic detection of Metallo-β-Lactamase in Imipenem resistant Pseudomonas aeruginosa. The Scientific World Journal <http://eprints.um.edu.my/view/publication/The_Scientific_World_Journal.html>. pp. 1-8. http://eprints.um.edu.my/10498/ |
| repository_type |
Digital Repository |
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Local University |
| institution |
University Malaya |
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UM Research Repository |
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Online Access |
| topic |
Q Science (General) R Medicine (General) |
| spellingShingle |
Q Science (General) R Medicine (General) Khosravi, Y. Loke, M.F. Tay, S.T. Vadivelu, J. Phenotypic detection of Metallo-β-Lactamase in Imipenem resistant Pseudomonas aeruginosa |
| description |
Carbapenems are the primary choice of treatment for severe Pseudomonas aeruginosa infection. However, the emergence of
carbapenem resistance due to the production of metallo β-lactamases (MBLs) is of global concern. In this study, 90 imipenem-(IPM- or IP-) resistant P. aeruginosa (IRPA) isolates, including 32 previously tested positive and genotyped for MBL genes by PCR, were subjected to double-disk synergy test (DDST), combined disk test (CDT), and imipenem/imipenem-inhibitor (IP/IPI) E-test to evaluate their MBLs detection capability. All three methods were shown to have a sensitivity of 100%. However, DDST was the most specific of the three (96.6%), followed by IP/IPI E-test interpreted based on the single criteria of IP/IPI ≥ 8 as positive (62.1%), and CDT was the least specific (43.1%). Based on the data from this evaluation, we propose that only IRPA with IP MIC > 16 μg/mL and IP/IPI ≥ 8 by IP/IPI E-test should be taken as positive for MBL activity.With the new dual interpretation criteria,the MBL IP/IPI E-test was shown to achieve 100% sensitivity as well as specificity for the IRPA in this study. Therefore, the IP/IPI E-test is a viable alternative phenotypic assay to detect MBL production in IRPA in our population in circumstances where PCR detection is not a feasible option. |
| format |
Article |
| author |
Khosravi, Y. Loke, M.F. Tay, S.T. Vadivelu, J. |
| author_facet |
Khosravi, Y. Loke, M.F. Tay, S.T. Vadivelu, J. |
| author_sort |
Khosravi, Y. |
| title |
Phenotypic detection of Metallo-β-Lactamase in Imipenem resistant Pseudomonas aeruginosa |
| title_short |
Phenotypic detection of Metallo-β-Lactamase in Imipenem resistant Pseudomonas aeruginosa |
| title_full |
Phenotypic detection of Metallo-β-Lactamase in Imipenem resistant Pseudomonas aeruginosa |
| title_fullStr |
Phenotypic detection of Metallo-β-Lactamase in Imipenem resistant Pseudomonas aeruginosa |
| title_full_unstemmed |
Phenotypic detection of Metallo-β-Lactamase in Imipenem resistant Pseudomonas aeruginosa |
| title_sort |
phenotypic detection of metallo-β-lactamase in imipenem resistant pseudomonas aeruginosa |
| publishDate |
2012 |
| url |
http://eprints.um.edu.my/10498/ http://eprints.um.edu.my/10498/1/Phenotypic_Detection_of_Metallo%2D%CE%B2%2DLactamase_in_Imipenem%2DResistant_Pseudomonas_aeruginosa.pdf |
| first_indexed |
2018-09-06T05:50:48Z |
| last_indexed |
2018-09-06T05:50:48Z |
| _version_ |
1610836133333172224 |