A method for rapidly screening functionality of actin mutants and tagged actins
Recombinant production and biochemical analysis of actin mutants has been hampered by the fact that actin has an absolute requirement for the eukaryotic chaperone CCT to reach its native state. We therefore have developed a method to rapidly screen the folding capacity and functionality of actin var...
| Main Authors: | , , , , |
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| Format: | Online |
| Language: | English |
| Published: |
Biological Procedures Online
2004
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC524212/ |