Analysis of the Damage Mechanism Related to CO2 Laser Cochleostomy on Guinea Pig Cochlea

Different types of lasers have been used in inner ear surgery. Therefore, it is of the utmost importance to avoid damage to the inner ear (e.g., hyperthermia and acoustic effects) caused by the use of such lasers. The aim of this study was to use a high powered fibre-enabled CO2 laser (10 W, 606 J/c...

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Main Authors:	Liu, Xiang, Qian, Xiao-qing, Ma, Rui, Chi, Fang-Lu, Ren, Dong-Dong
Format:	Online
Language:	English
Published:	Hindawi Publishing Corporation 2016
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5192333/

id	pubmed-5192333
recordtype	oai_dc
spelling	pubmed-51923332017-01-09 Analysis of the Damage Mechanism Related to CO2 Laser Cochleostomy on Guinea Pig Cochlea Liu, Xiang Qian, Xiao-qing Ma, Rui Chi, Fang-Lu Ren, Dong-Dong Research Article Different types of lasers have been used in inner ear surgery. Therefore, it is of the utmost importance to avoid damage to the inner ear (e.g., hyperthermia and acoustic effects) caused by the use of such lasers. The aim of this study was to use a high powered fibre-enabled CO2 laser (10 W, 606 J/cm2) to perform cochleostomies on guinea pig cochlea and to investigate the possible laser-induced damage mechanisms. The temperature changes in the round window membrane, auditory evoked brainstem response, and morphological of the hair cells were measured and recorded before and after laser application. All of the outcomes differed in comparison with the control group. A rise in temperature and subsequent increased hearing loss were observed in animals that underwent surgery with a 10 W CO2 laser. These findings correlated with increased injury to the cochlear ultrastructure and a higher positive expression of E-cadherin and β-catenin in the damaged organ of Corti. We assume that enhanced cell-cell adhesion and the activated β-catenin-related canonical Wnt-signalling pathway may play a role in the protection of the cochlea to prevent further damage. Hindawi Publishing Corporation 2016 2016-12-14 /pmc/articles/PMC5192333/ /pubmed/28070426 http://dx.doi.org/10.1155/2016/5982397 Text en Copyright © 2016 Xiang Liu et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Liu, Xiang Qian, Xiao-qing Ma, Rui Chi, Fang-Lu Ren, Dong-Dong
spellingShingle	Liu, Xiang Qian, Xiao-qing Ma, Rui Chi, Fang-Lu Ren, Dong-Dong Analysis of the Damage Mechanism Related to CO2 Laser Cochleostomy on Guinea Pig Cochlea
author_facet	Liu, Xiang Qian, Xiao-qing Ma, Rui Chi, Fang-Lu Ren, Dong-Dong
author_sort	Liu, Xiang
title	Analysis of the Damage Mechanism Related to CO2 Laser Cochleostomy on Guinea Pig Cochlea
title_short	Analysis of the Damage Mechanism Related to CO2 Laser Cochleostomy on Guinea Pig Cochlea
title_full	Analysis of the Damage Mechanism Related to CO2 Laser Cochleostomy on Guinea Pig Cochlea
title_fullStr	Analysis of the Damage Mechanism Related to CO2 Laser Cochleostomy on Guinea Pig Cochlea
title_full_unstemmed	Analysis of the Damage Mechanism Related to CO2 Laser Cochleostomy on Guinea Pig Cochlea
title_sort	analysis of the damage mechanism related to co2 laser cochleostomy on guinea pig cochlea
description	Different types of lasers have been used in inner ear surgery. Therefore, it is of the utmost importance to avoid damage to the inner ear (e.g., hyperthermia and acoustic effects) caused by the use of such lasers. The aim of this study was to use a high powered fibre-enabled CO2 laser (10 W, 606 J/cm2) to perform cochleostomies on guinea pig cochlea and to investigate the possible laser-induced damage mechanisms. The temperature changes in the round window membrane, auditory evoked brainstem response, and morphological of the hair cells were measured and recorded before and after laser application. All of the outcomes differed in comparison with the control group. A rise in temperature and subsequent increased hearing loss were observed in animals that underwent surgery with a 10 W CO2 laser. These findings correlated with increased injury to the cochlear ultrastructure and a higher positive expression of E-cadherin and β-catenin in the damaged organ of Corti. We assume that enhanced cell-cell adhesion and the activated β-catenin-related canonical Wnt-signalling pathway may play a role in the protection of the cochlea to prevent further damage.
publisher	Hindawi Publishing Corporation
publishDate	2016
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5192333/
_version_	1613833696231555072

Analysis of the Damage Mechanism Related to CO2 Laser Cochleostomy on Guinea Pig Cochlea

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