A serum miRNA profile of human longevity: findings from the Baltimore Longitudinal Study of Aging (BLSA)

A serum miRNA profile of human longevity: findings from the Baltimore Longitudinal Study of Aging (BLSA)

In C. elegans, miRNAs are genetic biomarkers of aging. Similarly, multiple miRNAs are differentially expressed between younger and older persons, suggesting that miRNA-regulated biological mechanisms affecting aging are evolutionarily conserved. Previous human studies have not considered participant...

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Main Authors: Smith-Vikos, Thalyana, Liu, Zuyun, Parsons, Christine, Gorospe, Myriam, Ferrucci, Luigi, Gill, Thomas M., Slack, Frank J.
Format: Online
Language:English
Published: Impact Journals LLC 2016
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5191881/
id pubmed-5191881
recordtype oai_dc
spelling pubmed-51918812016-12-28 A serum miRNA profile of human longevity: findings from the Baltimore Longitudinal Study of Aging (BLSA) Smith-Vikos, Thalyana Liu, Zuyun Parsons, Christine Gorospe, Myriam Ferrucci, Luigi Gill, Thomas M. Slack, Frank J. Research Paper In C. elegans, miRNAs are genetic biomarkers of aging. Similarly, multiple miRNAs are differentially expressed between younger and older persons, suggesting that miRNA-regulated biological mechanisms affecting aging are evolutionarily conserved. Previous human studies have not considered participants' lifespans, a key factor in identifying biomarkers of aging. Using PCR arrays, we measured miRNA levels from serum samples obtained longitudinally at ages 50, 55, and 60 from 16 non-Hispanic males who had documented lifespans from 58 to 92. Numerous miRNAs showed significant changes in expression levels. At age 50, 24 miRNAs were significantly upregulated, and 73 were significantly downregulated in the long-lived subgroup (76-92 years) as compared with the short-lived subgroup (58-75 years). In long-lived participants, the most upregulated was miR-373-5p, while the most downregulated was miR-15b-5p. Longitudinally, significant Pearson correlations were observed between lifespan and expression of nine miRNAs (p value<0.05). Six of these nine miRNAs (miR-211-5p, 374a-5p, 340-3p, 376c-3p, 5095, 1225-3p) were also significantly up- or downregulated when comparing long-lived and short-lived participants. Twenty-four validated targets of these miRNAs encoded aging-associated proteins, including PARP1, IGF1R, and IGF2R. We propose that the expression profiles of the six miRNAs (miR-211-5p, 374a-5p, 340-3p, 376c-3p, 5095, and 1225-3p) may be useful biomarkers of aging. Impact Journals LLC 2016-11-07 /pmc/articles/PMC5191881/ /pubmed/27824314 http://dx.doi.org/10.18632/aging.101106 Text en Copyright: © 2016 Smith-Vikos et al. http://creativecommons.org/licenses/by/3.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) (CC-BY), which permits unrestricted use and redistribution provided that the original author and source are credited.
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author Smith-Vikos, Thalyana
Liu, Zuyun
Parsons, Christine
Gorospe, Myriam
Ferrucci, Luigi
Gill, Thomas M.
Slack, Frank J.
spellingShingle Smith-Vikos, Thalyana
Liu, Zuyun
Parsons, Christine
Gorospe, Myriam
Ferrucci, Luigi
Gill, Thomas M.
Slack, Frank J.
A serum miRNA profile of human longevity: findings from the Baltimore Longitudinal Study of Aging (BLSA)
author_facet Smith-Vikos, Thalyana
Liu, Zuyun
Parsons, Christine
Gorospe, Myriam
Ferrucci, Luigi
Gill, Thomas M.
Slack, Frank J.
author_sort Smith-Vikos, Thalyana
title A serum miRNA profile of human longevity: findings from the Baltimore Longitudinal Study of Aging (BLSA)
title_short A serum miRNA profile of human longevity: findings from the Baltimore Longitudinal Study of Aging (BLSA)
title_full A serum miRNA profile of human longevity: findings from the Baltimore Longitudinal Study of Aging (BLSA)
title_fullStr A serum miRNA profile of human longevity: findings from the Baltimore Longitudinal Study of Aging (BLSA)
title_full_unstemmed A serum miRNA profile of human longevity: findings from the Baltimore Longitudinal Study of Aging (BLSA)
title_sort serum mirna profile of human longevity: findings from the baltimore longitudinal study of aging (blsa)
description In C. elegans, miRNAs are genetic biomarkers of aging. Similarly, multiple miRNAs are differentially expressed between younger and older persons, suggesting that miRNA-regulated biological mechanisms affecting aging are evolutionarily conserved. Previous human studies have not considered participants' lifespans, a key factor in identifying biomarkers of aging. Using PCR arrays, we measured miRNA levels from serum samples obtained longitudinally at ages 50, 55, and 60 from 16 non-Hispanic males who had documented lifespans from 58 to 92. Numerous miRNAs showed significant changes in expression levels. At age 50, 24 miRNAs were significantly upregulated, and 73 were significantly downregulated in the long-lived subgroup (76-92 years) as compared with the short-lived subgroup (58-75 years). In long-lived participants, the most upregulated was miR-373-5p, while the most downregulated was miR-15b-5p. Longitudinally, significant Pearson correlations were observed between lifespan and expression of nine miRNAs (p value<0.05). Six of these nine miRNAs (miR-211-5p, 374a-5p, 340-3p, 376c-3p, 5095, 1225-3p) were also significantly up- or downregulated when comparing long-lived and short-lived participants. Twenty-four validated targets of these miRNAs encoded aging-associated proteins, including PARP1, IGF1R, and IGF2R. We propose that the expression profiles of the six miRNAs (miR-211-5p, 374a-5p, 340-3p, 376c-3p, 5095, and 1225-3p) may be useful biomarkers of aging.
publisher Impact Journals LLC
publishDate 2016
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5191881/
_version_ 1613833261357727744

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