Onco-miR-130 promotes cell proliferation and migration by targeting TGFβR2 in gastric cancer

MicroRNAs (miRNAs) have been proved to play crucial roles in tumorigenesis. TGFβ signal pathway abnormality is found in various cancers and correlates with tumor proliferation and metastasis. However, the mechanisms underlying the dys-regulation of TGFβR2 expression in GC have not been investigated...

Full description

Bibliographic Details
Main Authors:	Duan, Jingjing, Zhang, Haiyang, Qu, Yanjun, Deng, Ting, Huang, Dingzhi, Liu, Rui, Zhang, Le, Bai, Ming, Zhou, Likun, Ying, Guoguang, Ba, Yi
Format:	Online
Language:	English
Published:	Impact Journals LLC 2016
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5190115/

id	pubmed-5190115
recordtype	oai_dc
spelling	pubmed-51901152017-01-05 Onco-miR-130 promotes cell proliferation and migration by targeting TGFβR2 in gastric cancer Duan, Jingjing Zhang, Haiyang Qu, Yanjun Deng, Ting Huang, Dingzhi Liu, Rui Zhang, Le Bai, Ming Zhou, Likun Ying, Guoguang Ba, Yi Research Paper MicroRNAs (miRNAs) have been proved to play crucial roles in tumorigenesis. TGFβ signal pathway abnormality is found in various cancers and correlates with tumor proliferation and metastasis. However, the mechanisms underlying the dys-regulation of TGFβR2 expression in GC have not been investigated yet. In this study, we found that the TGFβR2 protein was clearly repressed in tumor tissues, while miR-130 expression level was dramatically increased in GC tissues. Firefly luciferase activity assay revealed that miR-130 could directly bind to 3′UTR of TGFβR2 mRNA. Meanwhile, miR-130 mimics lead to the decreased TGFβR2 protein levels, while miR-130 inhibitors enhanced TGFβR2 expression in SGC7901 cells. Subsequent functional experiments showed that overexpressed miR-130 could promote proliferation and migration of SGC7901 cells. And siRNA-mediated TGFβR2 down-regulation could simulate the effects of miR-130 mimics on phenotypes of SGC7901 cells. Furthermore, there existed intense relationship between the expression level of miR-130 and epithelial-mesenchymal markers. Our results demonstrated that miR-130 was an oncogene by directly targeting TGFβR2 in GC. Impact Journals LLC 2016-06-10 /pmc/articles/PMC5190115/ /pubmed/27304191 http://dx.doi.org/10.18632/oncotarget.9936 Text en Copyright: © 2016 Duan et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Duan, Jingjing Zhang, Haiyang Qu, Yanjun Deng, Ting Huang, Dingzhi Liu, Rui Zhang, Le Bai, Ming Zhou, Likun Ying, Guoguang Ba, Yi
spellingShingle	Duan, Jingjing Zhang, Haiyang Qu, Yanjun Deng, Ting Huang, Dingzhi Liu, Rui Zhang, Le Bai, Ming Zhou, Likun Ying, Guoguang Ba, Yi Onco-miR-130 promotes cell proliferation and migration by targeting TGFβR2 in gastric cancer
author_facet	Duan, Jingjing Zhang, Haiyang Qu, Yanjun Deng, Ting Huang, Dingzhi Liu, Rui Zhang, Le Bai, Ming Zhou, Likun Ying, Guoguang Ba, Yi
author_sort	Duan, Jingjing
title	Onco-miR-130 promotes cell proliferation and migration by targeting TGFβR2 in gastric cancer
title_short	Onco-miR-130 promotes cell proliferation and migration by targeting TGFβR2 in gastric cancer
title_full	Onco-miR-130 promotes cell proliferation and migration by targeting TGFβR2 in gastric cancer
title_fullStr	Onco-miR-130 promotes cell proliferation and migration by targeting TGFβR2 in gastric cancer
title_full_unstemmed	Onco-miR-130 promotes cell proliferation and migration by targeting TGFβR2 in gastric cancer
title_sort	onco-mir-130 promotes cell proliferation and migration by targeting tgfβr2 in gastric cancer
description	MicroRNAs (miRNAs) have been proved to play crucial roles in tumorigenesis. TGFβ signal pathway abnormality is found in various cancers and correlates with tumor proliferation and metastasis. However, the mechanisms underlying the dys-regulation of TGFβR2 expression in GC have not been investigated yet. In this study, we found that the TGFβR2 protein was clearly repressed in tumor tissues, while miR-130 expression level was dramatically increased in GC tissues. Firefly luciferase activity assay revealed that miR-130 could directly bind to 3′UTR of TGFβR2 mRNA. Meanwhile, miR-130 mimics lead to the decreased TGFβR2 protein levels, while miR-130 inhibitors enhanced TGFβR2 expression in SGC7901 cells. Subsequent functional experiments showed that overexpressed miR-130 could promote proliferation and migration of SGC7901 cells. And siRNA-mediated TGFβR2 down-regulation could simulate the effects of miR-130 mimics on phenotypes of SGC7901 cells. Furthermore, there existed intense relationship between the expression level of miR-130 and epithelial-mesenchymal markers. Our results demonstrated that miR-130 was an oncogene by directly targeting TGFβR2 in GC.
publisher	Impact Journals LLC
publishDate	2016
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5190115/
_version_	1613830272914030592

Onco-miR-130 promotes cell proliferation and migration by targeting TGFβR2 in gastric cancer

Similar Items