Validating Intravascular Imaging with Serial Optical Coherence Tomography and Confocal Fluorescence Microscopy

Atherosclerotic cardiovascular diseases are characterized by the formation of a plaque in the arterial wall. Intravascular ultrasound (IVUS) provides high-resolution images allowing delineation of atherosclerotic plaques. When combined with near infrared fluorescence (NIRF), the plaque can also be s...

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Main Authors:	Tardif, Pier-Luc, Bertrand, Marie-Jeanne, Abran, Maxime, Castonguay, Alexandre, Lefebvre, Joël, Stähli, Barbara E., Merlet, Nolwenn, Mihalache-Avram, Teodora, Geoffroy, Pascale, Mecteau, Mélanie, Busseuil, David, Ni, Feng, Abulrob, Abedelnasser, Rhéaume, Éric, L’Allier, Philippe, Tardif, Jean-Claude, Lesage, Frédéric
Format:	Online
Language:	English
Published:	MDPI 2016
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5187910/

id	pubmed-5187910
recordtype	oai_dc
spelling	pubmed-51879102016-12-30 Validating Intravascular Imaging with Serial Optical Coherence Tomography and Confocal Fluorescence Microscopy Tardif, Pier-Luc Bertrand, Marie-Jeanne Abran, Maxime Castonguay, Alexandre Lefebvre, Joël Stähli, Barbara E. Merlet, Nolwenn Mihalache-Avram, Teodora Geoffroy, Pascale Mecteau, Mélanie Busseuil, David Ni, Feng Abulrob, Abedelnasser Rhéaume, Éric L’Allier, Philippe Tardif, Jean-Claude Lesage, Frédéric Article Atherosclerotic cardiovascular diseases are characterized by the formation of a plaque in the arterial wall. Intravascular ultrasound (IVUS) provides high-resolution images allowing delineation of atherosclerotic plaques. When combined with near infrared fluorescence (NIRF), the plaque can also be studied at a molecular level with a large variety of biomarkers. In this work, we present a system enabling automated volumetric histology imaging of excised aortas that can spatially correlate results with combined IVUS/NIRF imaging of lipid-rich atheroma in cholesterol-fed rabbits. Pullbacks in the rabbit aortas were performed with a dual modality IVUS/NIRF catheter developed by our group. Ex vivo three-dimensional (3D) histology was performed combining optical coherence tomography (OCT) and confocal fluorescence microscopy, providing high-resolution anatomical and molecular information, respectively, to validate in vivo findings. The microscope was combined with a serial slicer allowing for the imaging of the whole vessel automatically. Colocalization of in vivo and ex vivo results is demonstrated. Slices can then be recovered to be tested in conventional histology. MDPI 2016-12-15 /pmc/articles/PMC5187910/ /pubmed/27983695 http://dx.doi.org/10.3390/ijms17122110 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Tardif, Pier-Luc Bertrand, Marie-Jeanne Abran, Maxime Castonguay, Alexandre Lefebvre, Joël Stähli, Barbara E. Merlet, Nolwenn Mihalache-Avram, Teodora Geoffroy, Pascale Mecteau, Mélanie Busseuil, David Ni, Feng Abulrob, Abedelnasser Rhéaume, Éric L’Allier, Philippe Tardif, Jean-Claude Lesage, Frédéric
spellingShingle	Tardif, Pier-Luc Bertrand, Marie-Jeanne Abran, Maxime Castonguay, Alexandre Lefebvre, Joël Stähli, Barbara E. Merlet, Nolwenn Mihalache-Avram, Teodora Geoffroy, Pascale Mecteau, Mélanie Busseuil, David Ni, Feng Abulrob, Abedelnasser Rhéaume, Éric L’Allier, Philippe Tardif, Jean-Claude Lesage, Frédéric Validating Intravascular Imaging with Serial Optical Coherence Tomography and Confocal Fluorescence Microscopy
author_facet	Tardif, Pier-Luc Bertrand, Marie-Jeanne Abran, Maxime Castonguay, Alexandre Lefebvre, Joël Stähli, Barbara E. Merlet, Nolwenn Mihalache-Avram, Teodora Geoffroy, Pascale Mecteau, Mélanie Busseuil, David Ni, Feng Abulrob, Abedelnasser Rhéaume, Éric L’Allier, Philippe Tardif, Jean-Claude Lesage, Frédéric
author_sort	Tardif, Pier-Luc
title	Validating Intravascular Imaging with Serial Optical Coherence Tomography and Confocal Fluorescence Microscopy
title_short	Validating Intravascular Imaging with Serial Optical Coherence Tomography and Confocal Fluorescence Microscopy
title_full	Validating Intravascular Imaging with Serial Optical Coherence Tomography and Confocal Fluorescence Microscopy
title_fullStr	Validating Intravascular Imaging with Serial Optical Coherence Tomography and Confocal Fluorescence Microscopy
title_full_unstemmed	Validating Intravascular Imaging with Serial Optical Coherence Tomography and Confocal Fluorescence Microscopy
title_sort	validating intravascular imaging with serial optical coherence tomography and confocal fluorescence microscopy
description	Atherosclerotic cardiovascular diseases are characterized by the formation of a plaque in the arterial wall. Intravascular ultrasound (IVUS) provides high-resolution images allowing delineation of atherosclerotic plaques. When combined with near infrared fluorescence (NIRF), the plaque can also be studied at a molecular level with a large variety of biomarkers. In this work, we present a system enabling automated volumetric histology imaging of excised aortas that can spatially correlate results with combined IVUS/NIRF imaging of lipid-rich atheroma in cholesterol-fed rabbits. Pullbacks in the rabbit aortas were performed with a dual modality IVUS/NIRF catheter developed by our group. Ex vivo three-dimensional (3D) histology was performed combining optical coherence tomography (OCT) and confocal fluorescence microscopy, providing high-resolution anatomical and molecular information, respectively, to validate in vivo findings. The microscope was combined with a serial slicer allowing for the imaging of the whole vessel automatically. Colocalization of in vivo and ex vivo results is demonstrated. Slices can then be recovered to be tested in conventional histology.
publisher	MDPI
publishDate	2016
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5187910/
_version_	1613826591390957568

Validating Intravascular Imaging with Serial Optical Coherence Tomography and Confocal Fluorescence Microscopy

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