Relevance of MicroRNA200 Family and MicroRNA205 for Epithelial to Mesenchymal Transition and Clinical Outcome in Biliary Tract Cancer Patients

Extensive stromal interaction is one reason for the dismal outcome of biliary tract cancer (BTC) patients. Epithelial to mesenchymal transition (EMT) is involved in tumor invasion and metastasis and is partly regulated by microRNAs (miRs). This study explores the expression of anti-EMT miR200 family...

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Main Authors:	Urbas, Romana, Mayr, Christian, Klieser, Eckhard, Fuereder, Julia, Bach, Doris, Stättner, Stefan, Primavesi, Florian, Jaeger, Tarkan, Stanzer, Stefanie, Ress, Anna Lena, Löffelberger, Magdalena, Wagner, Andrej, Berr, Frieder, Ritter, Markus, Pichler, Martin, Neureiter, Daniel, Kiesslich, Tobias
Format:	Online
Language:	English
Published:	MDPI 2016
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5187853/

id	pubmed-5187853
recordtype	oai_dc
spelling	pubmed-51878532016-12-30 Relevance of MicroRNA200 Family and MicroRNA205 for Epithelial to Mesenchymal Transition and Clinical Outcome in Biliary Tract Cancer Patients Urbas, Romana Mayr, Christian Klieser, Eckhard Fuereder, Julia Bach, Doris Stättner, Stefan Primavesi, Florian Jaeger, Tarkan Stanzer, Stefanie Ress, Anna Lena Löffelberger, Magdalena Wagner, Andrej Berr, Frieder Ritter, Markus Pichler, Martin Neureiter, Daniel Kiesslich, Tobias Article Extensive stromal interaction is one reason for the dismal outcome of biliary tract cancer (BTC) patients. Epithelial to mesenchymal transition (EMT) is involved in tumor invasion and metastasis and is partly regulated by microRNAs (miRs). This study explores the expression of anti-EMT miR200 family (miR141, −200a/b/c, −429) and miR205 as well as the EMT-related proteins E-cadherin and vimentin in a panel of BTC cell lines and clinical specimens by quantitative real-time polymerase chain reaction, Western blot and immunohistochemistry, respectively. MicroRNA expression was correlated to (i) the expression patterns of E-cadherin and vimentin; (ii) clinicopathological characteristics; and (iii) survival data. MicroRNA-200 family and miR205 were expressed in all BTC cells and clinical specimens. E-cadherin and vimentin showed a mutually exclusive expression pattern in both, in vitro and in vivo. Expression of miR200 family members positively correlated with E-cadherin and negatively with vimentin expression in BTC cells and specimens. High expression of miR200 family members (but not miR205) and E-cadherin was associated with longer survival, while low miR200 family and high vimentin expression was a predictor of unfavorable survival. Overall, the current study demonstrates the relevance of the miR200 family in EMT of BTC tumors and suggests these miRs as predictors for positive outcome. MDPI 2016-12-07 /pmc/articles/PMC5187853/ /pubmed/27941621 http://dx.doi.org/10.3390/ijms17122053 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Urbas, Romana Mayr, Christian Klieser, Eckhard Fuereder, Julia Bach, Doris Stättner, Stefan Primavesi, Florian Jaeger, Tarkan Stanzer, Stefanie Ress, Anna Lena Löffelberger, Magdalena Wagner, Andrej Berr, Frieder Ritter, Markus Pichler, Martin Neureiter, Daniel Kiesslich, Tobias
spellingShingle	Urbas, Romana Mayr, Christian Klieser, Eckhard Fuereder, Julia Bach, Doris Stättner, Stefan Primavesi, Florian Jaeger, Tarkan Stanzer, Stefanie Ress, Anna Lena Löffelberger, Magdalena Wagner, Andrej Berr, Frieder Ritter, Markus Pichler, Martin Neureiter, Daniel Kiesslich, Tobias Relevance of MicroRNA200 Family and MicroRNA205 for Epithelial to Mesenchymal Transition and Clinical Outcome in Biliary Tract Cancer Patients
author_facet	Urbas, Romana Mayr, Christian Klieser, Eckhard Fuereder, Julia Bach, Doris Stättner, Stefan Primavesi, Florian Jaeger, Tarkan Stanzer, Stefanie Ress, Anna Lena Löffelberger, Magdalena Wagner, Andrej Berr, Frieder Ritter, Markus Pichler, Martin Neureiter, Daniel Kiesslich, Tobias
author_sort	Urbas, Romana
title	Relevance of MicroRNA200 Family and MicroRNA205 for Epithelial to Mesenchymal Transition and Clinical Outcome in Biliary Tract Cancer Patients
title_short	Relevance of MicroRNA200 Family and MicroRNA205 for Epithelial to Mesenchymal Transition and Clinical Outcome in Biliary Tract Cancer Patients
title_full	Relevance of MicroRNA200 Family and MicroRNA205 for Epithelial to Mesenchymal Transition and Clinical Outcome in Biliary Tract Cancer Patients
title_fullStr	Relevance of MicroRNA200 Family and MicroRNA205 for Epithelial to Mesenchymal Transition and Clinical Outcome in Biliary Tract Cancer Patients
title_full_unstemmed	Relevance of MicroRNA200 Family and MicroRNA205 for Epithelial to Mesenchymal Transition and Clinical Outcome in Biliary Tract Cancer Patients
title_sort	relevance of microrna200 family and microrna205 for epithelial to mesenchymal transition and clinical outcome in biliary tract cancer patients
description	Extensive stromal interaction is one reason for the dismal outcome of biliary tract cancer (BTC) patients. Epithelial to mesenchymal transition (EMT) is involved in tumor invasion and metastasis and is partly regulated by microRNAs (miRs). This study explores the expression of anti-EMT miR200 family (miR141, −200a/b/c, −429) and miR205 as well as the EMT-related proteins E-cadherin and vimentin in a panel of BTC cell lines and clinical specimens by quantitative real-time polymerase chain reaction, Western blot and immunohistochemistry, respectively. MicroRNA expression was correlated to (i) the expression patterns of E-cadherin and vimentin; (ii) clinicopathological characteristics; and (iii) survival data. MicroRNA-200 family and miR205 were expressed in all BTC cells and clinical specimens. E-cadherin and vimentin showed a mutually exclusive expression pattern in both, in vitro and in vivo. Expression of miR200 family members positively correlated with E-cadherin and negatively with vimentin expression in BTC cells and specimens. High expression of miR200 family members (but not miR205) and E-cadherin was associated with longer survival, while low miR200 family and high vimentin expression was a predictor of unfavorable survival. Overall, the current study demonstrates the relevance of the miR200 family in EMT of BTC tumors and suggests these miRs as predictors for positive outcome.
publisher	MDPI
publishDate	2016
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5187853/
_version_	1613826481636507648

Relevance of MicroRNA200 Family and MicroRNA205 for Epithelial to Mesenchymal Transition and Clinical Outcome in Biliary Tract Cancer Patients

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