ALV-J strain SCAU-HN06 induces innate immune responses in chicken primary monocyte-derived macrophages

ALV-J strain SCAU-HN06 induces innate immune responses in chicken primary monocyte-derived macrophages

Avian leucosis virus subgroup J (ALV-J) can cause lifelong infection and can escape from the host immune defenses in chickens. Since macrophages act as the important defense line against invading pathogens in host innate immunity, we investigated the function and innate immune responses of chicken p...

Full description

Bibliographic Details
Main Authors: Feng, Min, Dai, Manman, Cao, Weisheng, Tan, Yan, Li, Zhenhui, Shi, Meiqing, Zhang, Xiquan
Format: Online
Language:English
Published: Poultry Science Association, Inc. 2016
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5161024/
id pubmed-5161024
recordtype oai_dc
spelling pubmed-51610242016-12-19 ALV-J strain SCAU-HN06 induces innate immune responses in chicken primary monocyte-derived macrophages Feng, Min Dai, Manman Cao, Weisheng Tan, Yan Li, Zhenhui Shi, Meiqing Zhang, Xiquan Immunology, Health and Disease Avian leucosis virus subgroup J (ALV-J) can cause lifelong infection and can escape from the host immune defenses in chickens. Since macrophages act as the important defense line against invading pathogens in host innate immunity, we investigated the function and innate immune responses of chicken primary monocyte-derived macrophages (MDM) after ALV-J infection in this study. Our results indicated that ALV-J was stably maintained in MDM cells but that the viral growth rate was significantly lower than that in DF-1 cells. We also found that ALV-J infection significantly increased nitric oxide (NO) production, but had no effect on MDM phagocytic capacity. Interestingly, infection with ALV-J rapidly promoted the expression levels of Myxovirus resistance 1 (Mx) (3 h, 6 h), ISG12 (6 h), and interleukin-1β (IL-1β) (3 h, 12 h) at an early infection stage, whereas it sharply decreased the expression of Mx (24 h, 36 h), ISG12 (36 h), and made little change on IL-1β (24 h, 36 h) production at a late infection stage in MDM cells. Moreover, the protein levels of interferon-β (IFN-β) and interleukin-6 (IL-6) had sharply increased in infected MDM cells from 3 to 36 h post infection (hpi) of ALV-J. And, the protein level of interleukin-10 (IL-10) was dramatically decreased at 36 hpi in MDM cells infected with ALV-J. These results demonstrate that ALV-J can induce host innate immune responses and we hypothesize that macrophages play an important role in host innate immune attack and ALV-J immune escape. Poultry Science Association, Inc. 2016-08-02 2017-01 /pmc/articles/PMC5161024/ /pubmed/27486255 http://dx.doi.org/10.3382/ps/pew229 Text en © The Author 2016. Published by Oxford University Press on behalf of Poultry Science Association. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author Feng, Min
Dai, Manman
Cao, Weisheng
Tan, Yan
Li, Zhenhui
Shi, Meiqing
Zhang, Xiquan
spellingShingle Feng, Min
Dai, Manman
Cao, Weisheng
Tan, Yan
Li, Zhenhui
Shi, Meiqing
Zhang, Xiquan
ALV-J strain SCAU-HN06 induces innate immune responses in chicken primary monocyte-derived macrophages
author_facet Feng, Min
Dai, Manman
Cao, Weisheng
Tan, Yan
Li, Zhenhui
Shi, Meiqing
Zhang, Xiquan
author_sort Feng, Min
title ALV-J strain SCAU-HN06 induces innate immune responses in chicken primary monocyte-derived macrophages
title_short ALV-J strain SCAU-HN06 induces innate immune responses in chicken primary monocyte-derived macrophages
title_full ALV-J strain SCAU-HN06 induces innate immune responses in chicken primary monocyte-derived macrophages
title_fullStr ALV-J strain SCAU-HN06 induces innate immune responses in chicken primary monocyte-derived macrophages
title_full_unstemmed ALV-J strain SCAU-HN06 induces innate immune responses in chicken primary monocyte-derived macrophages
title_sort alv-j strain scau-hn06 induces innate immune responses in chicken primary monocyte-derived macrophages
description Avian leucosis virus subgroup J (ALV-J) can cause lifelong infection and can escape from the host immune defenses in chickens. Since macrophages act as the important defense line against invading pathogens in host innate immunity, we investigated the function and innate immune responses of chicken primary monocyte-derived macrophages (MDM) after ALV-J infection in this study. Our results indicated that ALV-J was stably maintained in MDM cells but that the viral growth rate was significantly lower than that in DF-1 cells. We also found that ALV-J infection significantly increased nitric oxide (NO) production, but had no effect on MDM phagocytic capacity. Interestingly, infection with ALV-J rapidly promoted the expression levels of Myxovirus resistance 1 (Mx) (3 h, 6 h), ISG12 (6 h), and interleukin-1β (IL-1β) (3 h, 12 h) at an early infection stage, whereas it sharply decreased the expression of Mx (24 h, 36 h), ISG12 (36 h), and made little change on IL-1β (24 h, 36 h) production at a late infection stage in MDM cells. Moreover, the protein levels of interferon-β (IFN-β) and interleukin-6 (IL-6) had sharply increased in infected MDM cells from 3 to 36 h post infection (hpi) of ALV-J. And, the protein level of interleukin-10 (IL-10) was dramatically decreased at 36 hpi in MDM cells infected with ALV-J. These results demonstrate that ALV-J can induce host innate immune responses and we hypothesize that macrophages play an important role in host innate immune attack and ALV-J immune escape.
publisher Poultry Science Association, Inc.
publishDate 2016
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5161024/
_version_ 1613786763697848320

Similar Items