Regulatory Role of OsMADS34 in the Determination of Glumes Fate, Grain Yield, and Quality in Rice
Grasses produce seeds on spikelets, a unique type of inflorescence. Despite the importance of grass crops for food, the genetic mechanisms that control spikelet development remain poorly understood. In this study, we used m34-z, a new mutant allele of the rice (Oryza sativa) E-class gene OsMADS34, t...
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| Format: | Online |
| Language: | English |
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Frontiers Media S.A.
2016
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5156729/ |
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pubmed-5156729 |
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pubmed-51567292016-12-23 Regulatory Role of OsMADS34 in the Determination of Glumes Fate, Grain Yield, and Quality in Rice Ren, Deyong Rao, Yuchun Leng, Yujia Li, Zizhuang Xu, Qiankun Wu, Liwen Qiu, Zhennan Xue, Dawei Zeng, Dali Hu, Jiang Zhang, Guangheng Zhu, Li Gao, Zhenyu Chen, Guang Dong, Guojun Guo, Longbiao Qian, Qian Plant Science Grasses produce seeds on spikelets, a unique type of inflorescence. Despite the importance of grass crops for food, the genetic mechanisms that control spikelet development remain poorly understood. In this study, we used m34-z, a new mutant allele of the rice (Oryza sativa) E-class gene OsMADS34, to examine OsMADS34 function in determining the identities of glumes (rudimentary glume and sterile lemma) and grain size. In the m34-z mutant, both the rudimentary glume and sterile lemma were homeotically converted to the lemma-like organ and acquired the lemma identity, suggesting that OsMADS34 plays important roles in the development of glumes. In the m34-z mutant, most of the grains from the secondary panicle branches (spb) were decreased in size, compared with grains from wild-type, but no differences were observed in the grains from the primary panicle branches. The amylose content and gel consistency, and a seed-setting rate from the spb were reduced in the m34-z mutant. Interesting, transcriptional activity analysis revealed that OsMADS34 protein was a transcription repressor and it may influence grain yield by suppressing the expressions of BG1, GW8, GW2, and GL7 in the m34-z mutant. These findings revealed that OsMADS34 largely affects grain yield by affecting the size of grains from the secondary branches. Frontiers Media S.A. 2016-12-15 /pmc/articles/PMC5156729/ /pubmed/28018389 http://dx.doi.org/10.3389/fpls.2016.01853 Text en Copyright © 2016 Ren, Rao, Leng, Li, Xu, Wu, Qiu, Xue, Zeng, Hu, Zhang, Zhu, Gao, Chen, Dong, Guo and Qian. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Ren, Deyong Rao, Yuchun Leng, Yujia Li, Zizhuang Xu, Qiankun Wu, Liwen Qiu, Zhennan Xue, Dawei Zeng, Dali Hu, Jiang Zhang, Guangheng Zhu, Li Gao, Zhenyu Chen, Guang Dong, Guojun Guo, Longbiao Qian, Qian |
| spellingShingle |
Ren, Deyong Rao, Yuchun Leng, Yujia Li, Zizhuang Xu, Qiankun Wu, Liwen Qiu, Zhennan Xue, Dawei Zeng, Dali Hu, Jiang Zhang, Guangheng Zhu, Li Gao, Zhenyu Chen, Guang Dong, Guojun Guo, Longbiao Qian, Qian Regulatory Role of OsMADS34 in the Determination of Glumes Fate, Grain Yield, and Quality in Rice |
| author_facet |
Ren, Deyong Rao, Yuchun Leng, Yujia Li, Zizhuang Xu, Qiankun Wu, Liwen Qiu, Zhennan Xue, Dawei Zeng, Dali Hu, Jiang Zhang, Guangheng Zhu, Li Gao, Zhenyu Chen, Guang Dong, Guojun Guo, Longbiao Qian, Qian |
| author_sort |
Ren, Deyong |
| title |
Regulatory Role of OsMADS34 in the Determination of Glumes Fate, Grain Yield, and Quality in Rice |
| title_short |
Regulatory Role of OsMADS34 in the Determination of Glumes Fate, Grain Yield, and Quality in Rice |
| title_full |
Regulatory Role of OsMADS34 in the Determination of Glumes Fate, Grain Yield, and Quality in Rice |
| title_fullStr |
Regulatory Role of OsMADS34 in the Determination of Glumes Fate, Grain Yield, and Quality in Rice |
| title_full_unstemmed |
Regulatory Role of OsMADS34 in the Determination of Glumes Fate, Grain Yield, and Quality in Rice |
| title_sort |
regulatory role of osmads34 in the determination of glumes fate, grain yield, and quality in rice |
| description |
Grasses produce seeds on spikelets, a unique type of inflorescence. Despite the importance of grass crops for food, the genetic mechanisms that control spikelet development remain poorly understood. In this study, we used m34-z, a new mutant allele of the rice (Oryza sativa) E-class gene OsMADS34, to examine OsMADS34 function in determining the identities of glumes (rudimentary glume and sterile lemma) and grain size. In the m34-z mutant, both the rudimentary glume and sterile lemma were homeotically converted to the lemma-like organ and acquired the lemma identity, suggesting that OsMADS34 plays important roles in the development of glumes. In the m34-z mutant, most of the grains from the secondary panicle branches (spb) were decreased in size, compared with grains from wild-type, but no differences were observed in the grains from the primary panicle branches. The amylose content and gel consistency, and a seed-setting rate from the spb were reduced in the m34-z mutant. Interesting, transcriptional activity analysis revealed that OsMADS34 protein was a transcription repressor and it may influence grain yield by suppressing the expressions of BG1, GW8, GW2, and GL7 in the m34-z mutant. These findings revealed that OsMADS34 largely affects grain yield by affecting the size of grains from the secondary branches. |
| publisher |
Frontiers Media S.A. |
| publishDate |
2016 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5156729/ |
| _version_ |
1613781235864174592 |