Detecting stoichiometry of macromolecular complexes in live cells using FRET

The stoichiometry of macromolecular interactions is fundamental to cellular signalling yet challenging to detect from living cells. Fluorescence resonance energy transfer (FRET) is a powerful phenomenon for characterizing close-range interactions whereby a donor fluorophore transfers energy to a clo...

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Bibliographic Details
Main Authors: Ben-Johny, Manu, Yue, Daniel N., Yue, David T.
Format: Online
Language:English
Published: Nature Publishing Group 2016
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5150656/