Tricholoma matsutake Aqueous Extract Induces Hepatocellular Carcinoma Cell Apoptosis via Caspase-Dependent Mitochondrial Pathway

Tricholoma matsutake Aqueous Extract Induces Hepatocellular Carcinoma Cell Apoptosis via Caspase-Dependent Mitochondrial Pathway

Tricholoma matsutake, one of widely accepted functional mushrooms, possesses various pharmacological activities, and its antitumor effect has become an important research point. Our study aims to evaluate the cytotoxicity activities of T. matsutake aqueous extract (TM) in HepG2 and SMMC-7721 cells....

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Main Authors: Wang, Yanzhen, Chen, Yiling, Zhang, Xinrui, Cai, Guangsheng, An, Shengshu, Wang, Xue, Teng, Lirong, Wang, Di
Format: Online
Language:English
Published: Hindawi Publishing Corporation 2016
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5149606/
id pubmed-5149606
recordtype oai_dc
spelling pubmed-51496062016-12-25 Tricholoma matsutake Aqueous Extract Induces Hepatocellular Carcinoma Cell Apoptosis via Caspase-Dependent Mitochondrial Pathway Wang, Yanzhen Chen, Yiling Zhang, Xinrui Cai, Guangsheng An, Shengshu Wang, Xue Teng, Lirong Wang, Di Research Article Tricholoma matsutake, one of widely accepted functional mushrooms, possesses various pharmacological activities, and its antitumor effect has become an important research point. Our study aims to evaluate the cytotoxicity activities of T. matsutake aqueous extract (TM) in HepG2 and SMMC-7721 cells. In in vitro experiments, TM strikingly reduced cell viability, promoted cell apoptosis, inhibited cell migration ability, induced excessive generation of ROS, and caused caspases cascade and mitochondrial membrane potential dissipation in hepatocellular carcinoma cells. In in vivo experiments, 14-day TM treatment strongly suppressed tumor growth in HepG2 and SMMC-7721-xenografted nude mice without influence on their body weights and liver function. Furthermore, TM increased the levels of cleaved poly-ADP-ribose polymerase (PARP), Bad, and Bax and reduced the expressions of B-cell lymphoma 2 (Bcl-2) in treated cells and tumor tissues. All aforementioned results suggest that caspase-dependent mitochondrial apoptotic pathways are involved in TM-mediated antihepatocellular carcinoma. Hindawi Publishing Corporation 2016 2016-11-28 /pmc/articles/PMC5149606/ /pubmed/28018916 http://dx.doi.org/10.1155/2016/9014364 Text en Copyright © 2016 Yanzhen Wang et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author Wang, Yanzhen
Chen, Yiling
Zhang, Xinrui
Cai, Guangsheng
An, Shengshu
Wang, Xue
Teng, Lirong
Wang, Di
spellingShingle Wang, Yanzhen
Chen, Yiling
Zhang, Xinrui
Cai, Guangsheng
An, Shengshu
Wang, Xue
Teng, Lirong
Wang, Di
Tricholoma matsutake Aqueous Extract Induces Hepatocellular Carcinoma Cell Apoptosis via Caspase-Dependent Mitochondrial Pathway
author_facet Wang, Yanzhen
Chen, Yiling
Zhang, Xinrui
Cai, Guangsheng
An, Shengshu
Wang, Xue
Teng, Lirong
Wang, Di
author_sort Wang, Yanzhen
title Tricholoma matsutake Aqueous Extract Induces Hepatocellular Carcinoma Cell Apoptosis via Caspase-Dependent Mitochondrial Pathway
title_short Tricholoma matsutake Aqueous Extract Induces Hepatocellular Carcinoma Cell Apoptosis via Caspase-Dependent Mitochondrial Pathway
title_full Tricholoma matsutake Aqueous Extract Induces Hepatocellular Carcinoma Cell Apoptosis via Caspase-Dependent Mitochondrial Pathway
title_fullStr Tricholoma matsutake Aqueous Extract Induces Hepatocellular Carcinoma Cell Apoptosis via Caspase-Dependent Mitochondrial Pathway
title_full_unstemmed Tricholoma matsutake Aqueous Extract Induces Hepatocellular Carcinoma Cell Apoptosis via Caspase-Dependent Mitochondrial Pathway
title_sort tricholoma matsutake aqueous extract induces hepatocellular carcinoma cell apoptosis via caspase-dependent mitochondrial pathway
description Tricholoma matsutake, one of widely accepted functional mushrooms, possesses various pharmacological activities, and its antitumor effect has become an important research point. Our study aims to evaluate the cytotoxicity activities of T. matsutake aqueous extract (TM) in HepG2 and SMMC-7721 cells. In in vitro experiments, TM strikingly reduced cell viability, promoted cell apoptosis, inhibited cell migration ability, induced excessive generation of ROS, and caused caspases cascade and mitochondrial membrane potential dissipation in hepatocellular carcinoma cells. In in vivo experiments, 14-day TM treatment strongly suppressed tumor growth in HepG2 and SMMC-7721-xenografted nude mice without influence on their body weights and liver function. Furthermore, TM increased the levels of cleaved poly-ADP-ribose polymerase (PARP), Bad, and Bax and reduced the expressions of B-cell lymphoma 2 (Bcl-2) in treated cells and tumor tissues. All aforementioned results suggest that caspase-dependent mitochondrial apoptotic pathways are involved in TM-mediated antihepatocellular carcinoma.
publisher Hindawi Publishing Corporation
publishDate 2016
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5149606/
_version_ 1613772435909246976

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