An integrated genomic approach for the study of mandibular prognathism in the European seabass (Dicentrarchus labrax)

An integrated genomic approach for the study of mandibular prognathism in the European seabass (Dicentrarchus labrax)

Skeletal anomalies in farmed fish are a relevant issue affecting animal welfare and health and causing significant economic losses. Here, a high-density genetic map of European seabass for QTL mapping of jaw deformity was constructed and a genome-wide association study (GWAS) was carried out on a to...

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Main Authors: Babbucci, Massimiliano, Ferraresso, Serena, Pauletto, Marianna, Franch, Rafaella, Papetti, Chiara, Patarnello, Tomaso, Carnier, Paolo, Bargelloni, Luca
Format: Online
Language:English
Published: Nature Publishing Group 2016
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5144136/
id pubmed-5144136
recordtype oai_dc
spelling pubmed-51441362016-12-16 An integrated genomic approach for the study of mandibular prognathism in the European seabass (Dicentrarchus labrax) Babbucci, Massimiliano Ferraresso, Serena Pauletto, Marianna Franch, Rafaella Papetti, Chiara Patarnello, Tomaso Carnier, Paolo Bargelloni, Luca Article Skeletal anomalies in farmed fish are a relevant issue affecting animal welfare and health and causing significant economic losses. Here, a high-density genetic map of European seabass for QTL mapping of jaw deformity was constructed and a genome-wide association study (GWAS) was carried out on a total of 298 juveniles, 148 of which belonged to four full-sib families. Out of 298 fish, 107 were affected by mandibular prognathism (MP). Three significant QTLs and two candidate SNPs associated with MP were identified. The two GWAS candidate markers were located on ChrX and Chr17, both in close proximity with the peaks of the two most significant QTLs. Notably, the SNP marker on Chr17 was positioned within the Sobp gene coding region, which plays a pivotal role in craniofacial development. The analysis of differentially expressed genes in jaw-deformed animals highlighted the “nervous system development” as a crucial pathway in MP. In particular, Zic2, a key gene for craniofacial morphogenesis in model species, was significantly down-regulated in MP-affected animals. Gene expression data revealed also a significant down-regulation of Sobp in deformed larvae. Our analyses, integrating transcriptomic and GWA methods, provide evidence for putative mechanisms underlying seabass jaw deformity. Nature Publishing Group 2016-12-08 /pmc/articles/PMC5144136/ /pubmed/27929136 http://dx.doi.org/10.1038/srep38673 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author Babbucci, Massimiliano
Ferraresso, Serena
Pauletto, Marianna
Franch, Rafaella
Papetti, Chiara
Patarnello, Tomaso
Carnier, Paolo
Bargelloni, Luca
spellingShingle Babbucci, Massimiliano
Ferraresso, Serena
Pauletto, Marianna
Franch, Rafaella
Papetti, Chiara
Patarnello, Tomaso
Carnier, Paolo
Bargelloni, Luca
An integrated genomic approach for the study of mandibular prognathism in the European seabass (Dicentrarchus labrax)
author_facet Babbucci, Massimiliano
Ferraresso, Serena
Pauletto, Marianna
Franch, Rafaella
Papetti, Chiara
Patarnello, Tomaso
Carnier, Paolo
Bargelloni, Luca
author_sort Babbucci, Massimiliano
title An integrated genomic approach for the study of mandibular prognathism in the European seabass (Dicentrarchus labrax)
title_short An integrated genomic approach for the study of mandibular prognathism in the European seabass (Dicentrarchus labrax)
title_full An integrated genomic approach for the study of mandibular prognathism in the European seabass (Dicentrarchus labrax)
title_fullStr An integrated genomic approach for the study of mandibular prognathism in the European seabass (Dicentrarchus labrax)
title_full_unstemmed An integrated genomic approach for the study of mandibular prognathism in the European seabass (Dicentrarchus labrax)
title_sort integrated genomic approach for the study of mandibular prognathism in the european seabass (dicentrarchus labrax)
description Skeletal anomalies in farmed fish are a relevant issue affecting animal welfare and health and causing significant economic losses. Here, a high-density genetic map of European seabass for QTL mapping of jaw deformity was constructed and a genome-wide association study (GWAS) was carried out on a total of 298 juveniles, 148 of which belonged to four full-sib families. Out of 298 fish, 107 were affected by mandibular prognathism (MP). Three significant QTLs and two candidate SNPs associated with MP were identified. The two GWAS candidate markers were located on ChrX and Chr17, both in close proximity with the peaks of the two most significant QTLs. Notably, the SNP marker on Chr17 was positioned within the Sobp gene coding region, which plays a pivotal role in craniofacial development. The analysis of differentially expressed genes in jaw-deformed animals highlighted the “nervous system development” as a crucial pathway in MP. In particular, Zic2, a key gene for craniofacial morphogenesis in model species, was significantly down-regulated in MP-affected animals. Gene expression data revealed also a significant down-regulation of Sobp in deformed larvae. Our analyses, integrating transcriptomic and GWA methods, provide evidence for putative mechanisms underlying seabass jaw deformity.
publisher Nature Publishing Group
publishDate 2016
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5144136/
_version_ 1613765302717251584

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