Augmented bronchial smooth muscle contractility induced by aqueous cigarette smoke extract in rats

Cigarette smoking is the main risk factor for the development of chronic obstructive pulmonary disease (COPD). However, little is known about the mechanisms of cigarette smoke-induced bronchial smooth muscle (BSM) hyperresponsiveness. In the present study, we investigated the effects of aqueous ciga...

Full description

Bibliographic Details
Main Authors:	Sakai, Hiroyasu, Watanabe, Ayako, Fujita, Akiko, Misawa, Miwa, Narita, Minoru, Chiba, Yoshihiko
Format:	Online
Language:	English
Published:	Japan Society of Smooth Muscle Research 2014
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5137307/

id	pubmed-5137307
recordtype	oai_dc
spelling	pubmed-51373072017-02-14 Augmented bronchial smooth muscle contractility induced by aqueous cigarette smoke extract in rats Sakai, Hiroyasu Watanabe, Ayako Fujita, Akiko Misawa, Miwa Narita, Minoru Chiba, Yoshihiko Original Cigarette smoking is the main risk factor for the development of chronic obstructive pulmonary disease (COPD). However, little is known about the mechanisms of cigarette smoke-induced bronchial smooth muscle (BSM) hyperresponsiveness. In the present study, we investigated the effects of aqueous cigarette smoke extract (ACSE) on the BSM contraction in rats. The bronchial strips of rats were incubated with ACSE or control-extract for 24 h. The acetylcholine (ACh), high K+ depolarization and sodium fluoride (NaF)-induced BSM contraction of the ACSE-treated group was significantly augmented as compared to that of the control one. The expression levels of both myosin light-chain kinase (MLCK) and RhoA were significantly increased in the ACSE-treated BSM. These findings suggest that the water-soluble components of cigarette smoke may cause BSM hyperresponsiveness via an increase in MLCK and RhoA. Japan Society of Smooth Muscle Research 2014-07-19 2014 /pmc/articles/PMC5137307/ http://dx.doi.org/10.1540/jsmr.50.39 Text en ©2014 The Japan Society of Smooth Muscle Research http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Sakai, Hiroyasu Watanabe, Ayako Fujita, Akiko Misawa, Miwa Narita, Minoru Chiba, Yoshihiko
spellingShingle	Sakai, Hiroyasu Watanabe, Ayako Fujita, Akiko Misawa, Miwa Narita, Minoru Chiba, Yoshihiko Augmented bronchial smooth muscle contractility induced by aqueous cigarette smoke extract in rats
author_facet	Sakai, Hiroyasu Watanabe, Ayako Fujita, Akiko Misawa, Miwa Narita, Minoru Chiba, Yoshihiko
author_sort	Sakai, Hiroyasu
title	Augmented bronchial smooth muscle contractility induced by aqueous cigarette smoke extract in rats
title_short	Augmented bronchial smooth muscle contractility induced by aqueous cigarette smoke extract in rats
title_full	Augmented bronchial smooth muscle contractility induced by aqueous cigarette smoke extract in rats
title_fullStr	Augmented bronchial smooth muscle contractility induced by aqueous cigarette smoke extract in rats
title_full_unstemmed	Augmented bronchial smooth muscle contractility induced by aqueous cigarette smoke extract in rats
title_sort	augmented bronchial smooth muscle contractility induced by aqueous cigarette smoke extract in rats
description	Cigarette smoking is the main risk factor for the development of chronic obstructive pulmonary disease (COPD). However, little is known about the mechanisms of cigarette smoke-induced bronchial smooth muscle (BSM) hyperresponsiveness. In the present study, we investigated the effects of aqueous cigarette smoke extract (ACSE) on the BSM contraction in rats. The bronchial strips of rats were incubated with ACSE or control-extract for 24 h. The acetylcholine (ACh), high K+ depolarization and sodium fluoride (NaF)-induced BSM contraction of the ACSE-treated group was significantly augmented as compared to that of the control one. The expression levels of both myosin light-chain kinase (MLCK) and RhoA were significantly increased in the ACSE-treated BSM. These findings suggest that the water-soluble components of cigarette smoke may cause BSM hyperresponsiveness via an increase in MLCK and RhoA.
publisher	Japan Society of Smooth Muscle Research
publishDate	2014
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5137307/
_version_	1613756878322401280

Augmented bronchial smooth muscle contractility induced by aqueous cigarette smoke extract in rats

Similar Items