Hormone Receptor Expression in Human Fascial Tissue
Many epidemiologic, clinical, and experimental findings point to sex differences in myofascial pain in view of the fact that adult women tend to have more myofascial problems with respect to men. It is possible that one of the stimuli to sensitization of fascial nociceptors could come from hormonal...
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| Format: | Online |
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PAGEPress Publications, Pavia, Italy
2016
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5134680/ |
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pubmed-51346802016-12-09 Hormone Receptor Expression in Human Fascial Tissue Fede, C. Albertin, G. Petrelli, L. Sfriso, M.M. Biz, C. De Caro, R. Stecco, C. Original Paper Many epidemiologic, clinical, and experimental findings point to sex differences in myofascial pain in view of the fact that adult women tend to have more myofascial problems with respect to men. It is possible that one of the stimuli to sensitization of fascial nociceptors could come from hormonal factors such as estrogen and relaxin, that are involved in extracellular matrix and collagen remodeling and thus contribute to functions of myofascial tissue. Immunohistochemical and molecular investigations (real-time PCR analysis) of relaxin receptor 1 (RXFP1) and estrogen receptor-alpha (ERα) localization were carried out on samples of human fascia collected from 8 volunteers patients during orthopedic surgery (all females, between 42 and 70 yrs, divided into pre- and post-menopausal groups), and in fibroblasts isolated from deep fascia, to examine both protein and RNA expression levels. We can assume that the two sex hormone receptors analyzed are expressed in all the human fascial districts examined and in fascial fibroblasts culture cells, to a lesser degree in the post-menopausal with respect to the pre-menopausal women. Hormone receptor expression was concentrated in the fibroblasts, and RXFP1 was also evident in blood vessels and nerves. Our results are the first demonstrating that the fibroblasts located within different districts of the muscular fasciae express sex hormone receptors and can help to explain the link between hormonal factors and myofascial pain. It is known, in fact, that estrogen and relaxin play a key role in extracellular matrix remodeling by inhibiting fibrosis and inflammatory activities, both important factors affecting fascial stiffness and sensitization of fascial nociceptors. PAGEPress Publications, Pavia, Italy 2016-11-02 /pmc/articles/PMC5134680/ /pubmed/28076930 http://dx.doi.org/10.4081/ejh.2016.2710 Text en ©Copyright C. Fede et al. http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Fede, C. Albertin, G. Petrelli, L. Sfriso, M.M. Biz, C. De Caro, R. Stecco, C. |
| spellingShingle |
Fede, C. Albertin, G. Petrelli, L. Sfriso, M.M. Biz, C. De Caro, R. Stecco, C. Hormone Receptor Expression in Human Fascial Tissue |
| author_facet |
Fede, C. Albertin, G. Petrelli, L. Sfriso, M.M. Biz, C. De Caro, R. Stecco, C. |
| author_sort |
Fede, C. |
| title |
Hormone Receptor Expression in Human Fascial Tissue |
| title_short |
Hormone Receptor Expression in Human Fascial Tissue |
| title_full |
Hormone Receptor Expression in Human Fascial Tissue |
| title_fullStr |
Hormone Receptor Expression in Human Fascial Tissue |
| title_full_unstemmed |
Hormone Receptor Expression in Human Fascial Tissue |
| title_sort |
hormone receptor expression in human fascial tissue |
| description |
Many epidemiologic, clinical, and experimental findings point to sex differences in myofascial pain in view of the fact that adult women tend to have more myofascial problems with respect to men. It is possible that one of the stimuli to sensitization of fascial nociceptors could come from hormonal factors such as estrogen and relaxin, that are involved in extracellular matrix and collagen remodeling and thus contribute to functions of myofascial tissue. Immunohistochemical and molecular investigations (real-time PCR analysis) of relaxin receptor 1 (RXFP1) and estrogen receptor-alpha (ERα) localization were carried out on samples of human fascia collected from 8 volunteers patients during orthopedic surgery (all females, between 42 and 70 yrs, divided into pre- and post-menopausal groups), and in fibroblasts isolated from deep fascia, to examine both protein and RNA expression levels. We can assume that the two sex hormone receptors analyzed are expressed in all the human fascial districts examined and in fascial fibroblasts culture cells, to a lesser degree in the post-menopausal with respect to the pre-menopausal women. Hormone receptor expression was concentrated in the fibroblasts, and RXFP1 was also evident in blood vessels and nerves. Our results are the first demonstrating that the fibroblasts located within different districts of the muscular fasciae express sex hormone receptors and can help to explain the link between hormonal factors and myofascial pain. It is known, in fact, that estrogen and relaxin play a key role in extracellular matrix remodeling by inhibiting fibrosis and inflammatory activities, both important factors affecting fascial stiffness and sensitization of fascial nociceptors. |
| publisher |
PAGEPress Publications, Pavia, Italy |
| publishDate |
2016 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5134680/ |
| _version_ |
1613754174836572160 |