In Vivo Follow-up of Brain Tumor Growth via Bioluminescence Imaging and Fluorescence Tomography

Reporter gene-based strategies are widely used in experimental oncology. Bioluminescence imaging (BLI) using the firefly luciferase (Fluc) as a reporter gene and d-luciferin as a substrate is currently the most widely employed technique. The present paper compares the performances of BLI imaging wit...

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Main Authors:	Genevois, Coralie, Loiseau, Hugues, Couillaud, Franck
Format:	Online
Language:	English
Published:	MDPI 2016
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5133816/

id	pubmed-5133816
recordtype	oai_dc
spelling	pubmed-51338162016-12-12 In Vivo Follow-up of Brain Tumor Growth via Bioluminescence Imaging and Fluorescence Tomography Genevois, Coralie Loiseau, Hugues Couillaud, Franck Article Reporter gene-based strategies are widely used in experimental oncology. Bioluminescence imaging (BLI) using the firefly luciferase (Fluc) as a reporter gene and d-luciferin as a substrate is currently the most widely employed technique. The present paper compares the performances of BLI imaging with fluorescence imaging using the near infrared fluorescent protein (iRFP) to monitor brain tumor growth in mice. Fluorescence imaging includes fluorescence reflectance imaging (FRI), fluorescence diffuse optical tomography (fDOT), and fluorescence molecular Imaging (FMT®). A U87 cell line was genetically modified for constitutive expression of both the encoding Fluc and iRFP reporter genes and assayed for cell, subcutaneous tumor and brain tumor imaging. On cultured cells, BLI was more sensitive than FRI; in vivo, tumors were first detected by BLI. Fluorescence of iRFP provided convenient tools such as flux cytometry, direct detection of the fluorescent protein on histological slices, and fluorescent tomography that allowed for 3D localization and absolute quantification of the fluorescent signal in brain tumors. MDPI 2016-10-31 /pmc/articles/PMC5133816/ /pubmed/27809256 http://dx.doi.org/10.3390/ijms17111815 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Genevois, Coralie Loiseau, Hugues Couillaud, Franck
spellingShingle	Genevois, Coralie Loiseau, Hugues Couillaud, Franck In Vivo Follow-up of Brain Tumor Growth via Bioluminescence Imaging and Fluorescence Tomography
author_facet	Genevois, Coralie Loiseau, Hugues Couillaud, Franck
author_sort	Genevois, Coralie
title	In Vivo Follow-up of Brain Tumor Growth via Bioluminescence Imaging and Fluorescence Tomography
title_short	In Vivo Follow-up of Brain Tumor Growth via Bioluminescence Imaging and Fluorescence Tomography
title_full	In Vivo Follow-up of Brain Tumor Growth via Bioluminescence Imaging and Fluorescence Tomography
title_fullStr	In Vivo Follow-up of Brain Tumor Growth via Bioluminescence Imaging and Fluorescence Tomography
title_full_unstemmed	In Vivo Follow-up of Brain Tumor Growth via Bioluminescence Imaging and Fluorescence Tomography
title_sort	in vivo follow-up of brain tumor growth via bioluminescence imaging and fluorescence tomography
description	Reporter gene-based strategies are widely used in experimental oncology. Bioluminescence imaging (BLI) using the firefly luciferase (Fluc) as a reporter gene and d-luciferin as a substrate is currently the most widely employed technique. The present paper compares the performances of BLI imaging with fluorescence imaging using the near infrared fluorescent protein (iRFP) to monitor brain tumor growth in mice. Fluorescence imaging includes fluorescence reflectance imaging (FRI), fluorescence diffuse optical tomography (fDOT), and fluorescence molecular Imaging (FMT®). A U87 cell line was genetically modified for constitutive expression of both the encoding Fluc and iRFP reporter genes and assayed for cell, subcutaneous tumor and brain tumor imaging. On cultured cells, BLI was more sensitive than FRI; in vivo, tumors were first detected by BLI. Fluorescence of iRFP provided convenient tools such as flux cytometry, direct detection of the fluorescent protein on histological slices, and fluorescent tomography that allowed for 3D localization and absolute quantification of the fluorescent signal in brain tumors.
publisher	MDPI
publishDate	2016
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5133816/
_version_	1613752999756169216

In Vivo Follow-up of Brain Tumor Growth via Bioluminescence Imaging and Fluorescence Tomography

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