Trio, a Rho Family GEF, Interacts with the Presynaptic Active Zone Proteins Piccolo and Bassoon

Synaptic vesicles (SVs) fuse with the plasma membrane at a precise location called the presynaptic active zone (AZ). This fusion is coordinated by proteins embedded within a cytoskeletal matrix assembled at the AZ (CAZ). In the present study, we have identified a novel binding partner for the CAZ pr...

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Main Authors:	Terry-Lorenzo, Ryan T., Torres, Viviana I., Wagh, Dhananjay, Galaz, Jose, Swanson, Selene K., Florens, Laurence, Washburn, Michael P., Waites, Clarissa L., Gundelfinger, Eckart D., Reimer, Richard J., Garner, Craig C.
Format:	Online
Language:	English
Published:	Public Library of Science 2016
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5132261/

id	pubmed-5132261
recordtype	oai_dc
spelling	pubmed-51322612016-12-21 Trio, a Rho Family GEF, Interacts with the Presynaptic Active Zone Proteins Piccolo and Bassoon Terry-Lorenzo, Ryan T. Torres, Viviana I. Wagh, Dhananjay Galaz, Jose Swanson, Selene K. Florens, Laurence Washburn, Michael P. Waites, Clarissa L. Gundelfinger, Eckart D. Reimer, Richard J. Garner, Craig C. Research Article Synaptic vesicles (SVs) fuse with the plasma membrane at a precise location called the presynaptic active zone (AZ). This fusion is coordinated by proteins embedded within a cytoskeletal matrix assembled at the AZ (CAZ). In the present study, we have identified a novel binding partner for the CAZ proteins Piccolo and Bassoon. This interacting protein, Trio, is a member of the Dbl family of guanine nucleotide exchange factors (GEFs) known to regulate the dynamic assembly of actin and growth factor dependent axon guidance and synaptic growth. Trio was found to interact with the C-terminal PBH 9/10 domains of Piccolo and Bassoon via its own N-terminal Spectrin repeats, a domain that is also critical for its localization to the CAZ. Moreover, our data suggest that regions within the C-terminus of Trio negatively regulate its interactions with Piccolo/Bassoon. These findings provide a mechanism for the presynaptic targeting of Trio and support a model in which Piccolo and Bassoon play a role in regulating neurotransmission through interactions with proteins, including Trio, that modulate the dynamic assembly of F-actin during cycles of synaptic vesicle exo- and endocytosis. Public Library of Science 2016-12-01 /pmc/articles/PMC5132261/ /pubmed/27907191 http://dx.doi.org/10.1371/journal.pone.0167535 Text en © 2016 Terry-Lorenzo et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Terry-Lorenzo, Ryan T. Torres, Viviana I. Wagh, Dhananjay Galaz, Jose Swanson, Selene K. Florens, Laurence Washburn, Michael P. Waites, Clarissa L. Gundelfinger, Eckart D. Reimer, Richard J. Garner, Craig C.
spellingShingle	Terry-Lorenzo, Ryan T. Torres, Viviana I. Wagh, Dhananjay Galaz, Jose Swanson, Selene K. Florens, Laurence Washburn, Michael P. Waites, Clarissa L. Gundelfinger, Eckart D. Reimer, Richard J. Garner, Craig C. Trio, a Rho Family GEF, Interacts with the Presynaptic Active Zone Proteins Piccolo and Bassoon
author_facet	Terry-Lorenzo, Ryan T. Torres, Viviana I. Wagh, Dhananjay Galaz, Jose Swanson, Selene K. Florens, Laurence Washburn, Michael P. Waites, Clarissa L. Gundelfinger, Eckart D. Reimer, Richard J. Garner, Craig C.
author_sort	Terry-Lorenzo, Ryan T.
title	Trio, a Rho Family GEF, Interacts with the Presynaptic Active Zone Proteins Piccolo and Bassoon
title_short	Trio, a Rho Family GEF, Interacts with the Presynaptic Active Zone Proteins Piccolo and Bassoon
title_full	Trio, a Rho Family GEF, Interacts with the Presynaptic Active Zone Proteins Piccolo and Bassoon
title_fullStr	Trio, a Rho Family GEF, Interacts with the Presynaptic Active Zone Proteins Piccolo and Bassoon
title_full_unstemmed	Trio, a Rho Family GEF, Interacts with the Presynaptic Active Zone Proteins Piccolo and Bassoon
title_sort	trio, a rho family gef, interacts with the presynaptic active zone proteins piccolo and bassoon
description	Synaptic vesicles (SVs) fuse with the plasma membrane at a precise location called the presynaptic active zone (AZ). This fusion is coordinated by proteins embedded within a cytoskeletal matrix assembled at the AZ (CAZ). In the present study, we have identified a novel binding partner for the CAZ proteins Piccolo and Bassoon. This interacting protein, Trio, is a member of the Dbl family of guanine nucleotide exchange factors (GEFs) known to regulate the dynamic assembly of actin and growth factor dependent axon guidance and synaptic growth. Trio was found to interact with the C-terminal PBH 9/10 domains of Piccolo and Bassoon via its own N-terminal Spectrin repeats, a domain that is also critical for its localization to the CAZ. Moreover, our data suggest that regions within the C-terminus of Trio negatively regulate its interactions with Piccolo/Bassoon. These findings provide a mechanism for the presynaptic targeting of Trio and support a model in which Piccolo and Bassoon play a role in regulating neurotransmission through interactions with proteins, including Trio, that modulate the dynamic assembly of F-actin during cycles of synaptic vesicle exo- and endocytosis.
publisher	Public Library of Science
publishDate	2016
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5132261/
_version_	1613750826009886720

Trio, a Rho Family GEF, Interacts with the Presynaptic Active Zone Proteins Piccolo and Bassoon

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