Investigating the Influence of MoS2 Nanosheets on E. coli from Metabolomics Level
Molybdenum disulfide, a type of two-dimensional layered material with unique properties, has been widely used in many fields. However, an exact understanding of its toxicity remains elusive, let alone its effects on the environmental microbial community. In this study, we utilized metabolomics techn...
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Public Library of Science
2016
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5132170/ |
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pubmed-51321702016-12-21 Investigating the Influence of MoS2 Nanosheets on E. coli from Metabolomics Level Wu, Na Yu, Yadong Li, Tao Ji, Xiaojun Jiang, Ling Zong, Jiajun Huang, He Research Article Molybdenum disulfide, a type of two-dimensional layered material with unique properties, has been widely used in many fields. However, an exact understanding of its toxicity remains elusive, let alone its effects on the environmental microbial community. In this study, we utilized metabolomics technology to explore the effects of different concentrations of molybdenum disulfide nanosheets on Escherichia coli for the first time. The results showed that with increasing concentration of molybdenum disulfide nanosheets, the survival rate of Escherichia coli was decreased and the release of lactic dehydrogenase was increased. At the same time, intracellular concentrations of reactive oxygen species were dramatically increased. In addition, metabolomics analysis showed that high concentrations of molybdenum disulfide nanosheets (100, 1000 μg/mL) could significantly affect the metabolic profile of Escherichia coli, including glycine, serine and threonine metabolism, protein biosynthesis, urea cycle and pyruvate metabolism. These results will be beneficial for molybdenum disulfide toxicity assessment and further applications. Public Library of Science 2016-12-01 /pmc/articles/PMC5132170/ /pubmed/27907068 http://dx.doi.org/10.1371/journal.pone.0167245 Text en © 2016 Wu et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Wu, Na Yu, Yadong Li, Tao Ji, Xiaojun Jiang, Ling Zong, Jiajun Huang, He |
| spellingShingle |
Wu, Na Yu, Yadong Li, Tao Ji, Xiaojun Jiang, Ling Zong, Jiajun Huang, He Investigating the Influence of MoS2 Nanosheets on E. coli from Metabolomics Level |
| author_facet |
Wu, Na Yu, Yadong Li, Tao Ji, Xiaojun Jiang, Ling Zong, Jiajun Huang, He |
| author_sort |
Wu, Na |
| title |
Investigating the Influence of MoS2 Nanosheets on E. coli from Metabolomics Level |
| title_short |
Investigating the Influence of MoS2 Nanosheets on E. coli from Metabolomics Level |
| title_full |
Investigating the Influence of MoS2 Nanosheets on E. coli from Metabolomics Level |
| title_fullStr |
Investigating the Influence of MoS2 Nanosheets on E. coli from Metabolomics Level |
| title_full_unstemmed |
Investigating the Influence of MoS2 Nanosheets on E. coli from Metabolomics Level |
| title_sort |
investigating the influence of mos2 nanosheets on e. coli from metabolomics level |
| description |
Molybdenum disulfide, a type of two-dimensional layered material with unique properties, has been widely used in many fields. However, an exact understanding of its toxicity remains elusive, let alone its effects on the environmental microbial community. In this study, we utilized metabolomics technology to explore the effects of different concentrations of molybdenum disulfide nanosheets on Escherichia coli for the first time. The results showed that with increasing concentration of molybdenum disulfide nanosheets, the survival rate of Escherichia coli was decreased and the release of lactic dehydrogenase was increased. At the same time, intracellular concentrations of reactive oxygen species were dramatically increased. In addition, metabolomics analysis showed that high concentrations of molybdenum disulfide nanosheets (100, 1000 μg/mL) could significantly affect the metabolic profile of Escherichia coli, including glycine, serine and threonine metabolism, protein biosynthesis, urea cycle and pyruvate metabolism. These results will be beneficial for molybdenum disulfide toxicity assessment and further applications. |
| publisher |
Public Library of Science |
| publishDate |
2016 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5132170/ |
| _version_ |
1613750688318226432 |