Paper‐Origami‐Based Multiplexed Malaria Diagnostics from Whole Blood

Paper‐Origami‐Based Multiplexed Malaria Diagnostics from Whole Blood

We demonstrate, for the first time, the multiplexed determination of microbial species from whole blood using the paper‐folding technique of origami to enable the sequential steps of DNA extraction, loop‐mediated isothermal amplification (LAMP), and array‐based fluorescence detection. A low‐cost han...

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Main Authors: Xu, Gaolian, Nolder, Debbie, Reboud, Julien, Oguike, Mary C., van Schalkwyk, Donelly A., Sutherland, Colin J., Cooper, Jonathan M.
Format: Online
Language:English
Published: John Wiley and Sons Inc. 2016
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5132111/
id pubmed-5132111
recordtype oai_dc
spelling pubmed-51321112016-12-02 Paper‐Origami‐Based Multiplexed Malaria Diagnostics from Whole Blood Xu, Gaolian Nolder, Debbie Reboud, Julien Oguike, Mary C. van Schalkwyk, Donelly A. Sutherland, Colin J. Cooper, Jonathan M. Communications We demonstrate, for the first time, the multiplexed determination of microbial species from whole blood using the paper‐folding technique of origami to enable the sequential steps of DNA extraction, loop‐mediated isothermal amplification (LAMP), and array‐based fluorescence detection. A low‐cost handheld flashlight reveals the presence of the final DNA amplicon to the naked eye, providing a “sample‐to‐answer” diagnosis from a finger‐prick volume of human blood, within 45 min, with minimal user intervention. To demonstrate the method, we showed the identification of three species of Plasmodium, analyzing 80 patient samples benchmarked against the gold‐standard polymerase chain reaction (PCR) assay in an operator‐blinded study. We also show that the test retains its diagnostic accuracy when using stored or fixed reference samples. John Wiley and Sons Inc. 2016-08-24 2016-12-05 /pmc/articles/PMC5132111/ /pubmed/27554333 http://dx.doi.org/10.1002/anie.201606060 Text en © 2016 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author Xu, Gaolian
Nolder, Debbie
Reboud, Julien
Oguike, Mary C.
van Schalkwyk, Donelly A.
Sutherland, Colin J.
Cooper, Jonathan M.
spellingShingle Xu, Gaolian
Nolder, Debbie
Reboud, Julien
Oguike, Mary C.
van Schalkwyk, Donelly A.
Sutherland, Colin J.
Cooper, Jonathan M.
Paper‐Origami‐Based Multiplexed Malaria Diagnostics from Whole Blood
author_facet Xu, Gaolian
Nolder, Debbie
Reboud, Julien
Oguike, Mary C.
van Schalkwyk, Donelly A.
Sutherland, Colin J.
Cooper, Jonathan M.
author_sort Xu, Gaolian
title Paper‐Origami‐Based Multiplexed Malaria Diagnostics from Whole Blood
title_short Paper‐Origami‐Based Multiplexed Malaria Diagnostics from Whole Blood
title_full Paper‐Origami‐Based Multiplexed Malaria Diagnostics from Whole Blood
title_fullStr Paper‐Origami‐Based Multiplexed Malaria Diagnostics from Whole Blood
title_full_unstemmed Paper‐Origami‐Based Multiplexed Malaria Diagnostics from Whole Blood
title_sort paper‐origami‐based multiplexed malaria diagnostics from whole blood
description We demonstrate, for the first time, the multiplexed determination of microbial species from whole blood using the paper‐folding technique of origami to enable the sequential steps of DNA extraction, loop‐mediated isothermal amplification (LAMP), and array‐based fluorescence detection. A low‐cost handheld flashlight reveals the presence of the final DNA amplicon to the naked eye, providing a “sample‐to‐answer” diagnosis from a finger‐prick volume of human blood, within 45 min, with minimal user intervention. To demonstrate the method, we showed the identification of three species of Plasmodium, analyzing 80 patient samples benchmarked against the gold‐standard polymerase chain reaction (PCR) assay in an operator‐blinded study. We also show that the test retains its diagnostic accuracy when using stored or fixed reference samples.
publisher John Wiley and Sons Inc.
publishDate 2016
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5132111/
_version_ 1613750591847137280

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