Multiplex 16S rRNA‐derived geno‐biochip for detection of 16 bacterial pathogens from contaminated foods

Foodborne diseases caused by various pathogenic bacteria occur worldwide. To prevent foodborne diseases and minimize their impacts, it is important to inspect contaminated foods and specifically detect many types of pathogenic bacteria. Several DNA oligonucleotide biochips based on 16S rRNA have bee...

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Main Authors:	Shin, Hwa Hui, Hwang, Byeong Hee, Cha, Hyung Joon
Format:	Online
Language:	English
Published:	WILEY‐VCH Verlag 2016
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5132059/

id	pubmed-5132059
recordtype	oai_dc
spelling	pubmed-51320592016-12-02 Multiplex 16S rRNA‐derived geno‐biochip for detection of 16 bacterial pathogens from contaminated foods Shin, Hwa Hui Hwang, Byeong Hee Cha, Hyung Joon Research Articles Foodborne diseases caused by various pathogenic bacteria occur worldwide. To prevent foodborne diseases and minimize their impacts, it is important to inspect contaminated foods and specifically detect many types of pathogenic bacteria. Several DNA oligonucleotide biochips based on 16S rRNA have been investigated to detect bacteria; however, a mode of detection that can be used to detect diverse pathogenic strains and to examine the safety of food matrixes is still needed. In the present work, a 16S rRNA gene‐derived geno‐biochip detection system was developed after screening DNA oligonucleotide specific capture probes, and it was validated for multiple detection of 16 pathogenic strains that frequently occur with a signature pattern. rRNAs were also used as detection targets directly obtained from cell lysates without any purification and amplification steps in the bacterial cells separated from 8 food matrixes by simple pretreatments. Thus, the developed 16S rRNA‐derived geno‐biochip can be successfully used for the rapid and multiple detection of the 16 pathogenic bacteria frequently isolated from contaminated foods that are important for food safety. WILEY‐VCH Verlag 2016-09-06 2016-11 /pmc/articles/PMC5132059/ /pubmed/27492058 http://dx.doi.org/10.1002/biot.201600043 Text en © 2016 The Authors. Biotechnology Journal published by WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Shin, Hwa Hui Hwang, Byeong Hee Cha, Hyung Joon
spellingShingle	Shin, Hwa Hui Hwang, Byeong Hee Cha, Hyung Joon Multiplex 16S rRNA‐derived geno‐biochip for detection of 16 bacterial pathogens from contaminated foods
author_facet	Shin, Hwa Hui Hwang, Byeong Hee Cha, Hyung Joon
author_sort	Shin, Hwa Hui
title	Multiplex 16S rRNA‐derived geno‐biochip for detection of 16 bacterial pathogens from contaminated foods
title_short	Multiplex 16S rRNA‐derived geno‐biochip for detection of 16 bacterial pathogens from contaminated foods
title_full	Multiplex 16S rRNA‐derived geno‐biochip for detection of 16 bacterial pathogens from contaminated foods
title_fullStr	Multiplex 16S rRNA‐derived geno‐biochip for detection of 16 bacterial pathogens from contaminated foods
title_full_unstemmed	Multiplex 16S rRNA‐derived geno‐biochip for detection of 16 bacterial pathogens from contaminated foods
title_sort	multiplex 16s rrna‐derived geno‐biochip for detection of 16 bacterial pathogens from contaminated foods
description	Foodborne diseases caused by various pathogenic bacteria occur worldwide. To prevent foodborne diseases and minimize their impacts, it is important to inspect contaminated foods and specifically detect many types of pathogenic bacteria. Several DNA oligonucleotide biochips based on 16S rRNA have been investigated to detect bacteria; however, a mode of detection that can be used to detect diverse pathogenic strains and to examine the safety of food matrixes is still needed. In the present work, a 16S rRNA gene‐derived geno‐biochip detection system was developed after screening DNA oligonucleotide specific capture probes, and it was validated for multiple detection of 16 pathogenic strains that frequently occur with a signature pattern. rRNAs were also used as detection targets directly obtained from cell lysates without any purification and amplification steps in the bacterial cells separated from 8 food matrixes by simple pretreatments. Thus, the developed 16S rRNA‐derived geno‐biochip can be successfully used for the rapid and multiple detection of the 16 pathogenic bacteria frequently isolated from contaminated foods that are important for food safety.
publisher	WILEY‐VCH Verlag
publishDate	2016
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5132059/
_version_	1613750515496124416

Multiplex 16S rRNA‐derived geno‐biochip for detection of 16 bacterial pathogens from contaminated foods

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