Picroside II Attenuates Airway Inflammation by Downregulating the Transcription Factor GATA3 and Th2-Related Cytokines in a Mouse Model of HDM-Induced Allergic Asthma

Picroside II isolated from Pseudolysimachion rotundum var. subintegrum has been used as traditional medicine to treat inflammatory diseases. In this study, we assessed whether picroside II has inhibitory effects on airway inflammation in a mouse model of house dust mite (HDM)-induced asthma. In the...

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Main Authors:	Choi, Jin, Choi, Bo Kyong, Kim, Jin seok, Lee, Jae-Won, Park, Hyun Ah, Ryu, Hyung Won, Lee, Su Ui, Hwang, Kwang Woo, Yun, Won-Kee, Kim, Hyoung-Chin, Ahn, Kyung-Seop, Oh, Sei-Ryang, Lee, Hyun-Jun
Format:	Online
Language:	English
Published:	Public Library of Science 2016
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5117775/

id	pubmed-5117775
recordtype	oai_dc
spelling	pubmed-51177752016-12-15 Picroside II Attenuates Airway Inflammation by Downregulating the Transcription Factor GATA3 and Th2-Related Cytokines in a Mouse Model of HDM-Induced Allergic Asthma Choi, Jin Choi, Bo Kyong Kim, Jin seok Lee, Jae-Won Park, Hyun Ah Ryu, Hyung Won Lee, Su Ui Hwang, Kwang Woo Yun, Won-Kee Kim, Hyoung-Chin Ahn, Kyung-Seop Oh, Sei-Ryang Lee, Hyun-Jun Research Article Picroside II isolated from Pseudolysimachion rotundum var. subintegrum has been used as traditional medicine to treat inflammatory diseases. In this study, we assessed whether picroside II has inhibitory effects on airway inflammation in a mouse model of house dust mite (HDM)-induced asthma. In the HDM-induced asthmatic model, picroside II significantly reduced inflammatory cell counts in the bronchoalveolar lavage fluid (BALF), the levels of total immunoglobulin (Ig) E and HDM-specific IgE and IgG1 in serum, airway inflammation, and mucus hypersecretion in the lung tissues. ELISA analysis showed that picroside II down-regulated the levels of Th2-related cytokines (including IL-4, IL-5, and IL-13) and asthma-related mediators, but it up-regulated Th1-related cytokine, IFNγ in BALF. Picroside II also inhibited the expression of Th2 type cytokine genes and the transcription factor GATA3 in the lung tissues of HDM-induced mice. Finally, we demonstrated that picroside II significantly decreased the expression of GATA3 and Th2 cytokines in developing Th2 cells, consistent with in vivo results. Taken together, these results indicate that picroside II has protective effects on allergic asthma by reducing GATA3 expression and Th2 cytokine bias. Public Library of Science 2016-11-21 /pmc/articles/PMC5117775/ /pubmed/27870920 http://dx.doi.org/10.1371/journal.pone.0167098 Text en © 2016 Choi et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Choi, Jin Choi, Bo Kyong Kim, Jin seok Lee, Jae-Won Park, Hyun Ah Ryu, Hyung Won Lee, Su Ui Hwang, Kwang Woo Yun, Won-Kee Kim, Hyoung-Chin Ahn, Kyung-Seop Oh, Sei-Ryang Lee, Hyun-Jun
spellingShingle	Choi, Jin Choi, Bo Kyong Kim, Jin seok Lee, Jae-Won Park, Hyun Ah Ryu, Hyung Won Lee, Su Ui Hwang, Kwang Woo Yun, Won-Kee Kim, Hyoung-Chin Ahn, Kyung-Seop Oh, Sei-Ryang Lee, Hyun-Jun Picroside II Attenuates Airway Inflammation by Downregulating the Transcription Factor GATA3 and Th2-Related Cytokines in a Mouse Model of HDM-Induced Allergic Asthma
author_facet	Choi, Jin Choi, Bo Kyong Kim, Jin seok Lee, Jae-Won Park, Hyun Ah Ryu, Hyung Won Lee, Su Ui Hwang, Kwang Woo Yun, Won-Kee Kim, Hyoung-Chin Ahn, Kyung-Seop Oh, Sei-Ryang Lee, Hyun-Jun
author_sort	Choi, Jin
title	Picroside II Attenuates Airway Inflammation by Downregulating the Transcription Factor GATA3 and Th2-Related Cytokines in a Mouse Model of HDM-Induced Allergic Asthma
title_short	Picroside II Attenuates Airway Inflammation by Downregulating the Transcription Factor GATA3 and Th2-Related Cytokines in a Mouse Model of HDM-Induced Allergic Asthma
title_full	Picroside II Attenuates Airway Inflammation by Downregulating the Transcription Factor GATA3 and Th2-Related Cytokines in a Mouse Model of HDM-Induced Allergic Asthma
title_fullStr	Picroside II Attenuates Airway Inflammation by Downregulating the Transcription Factor GATA3 and Th2-Related Cytokines in a Mouse Model of HDM-Induced Allergic Asthma
title_full_unstemmed	Picroside II Attenuates Airway Inflammation by Downregulating the Transcription Factor GATA3 and Th2-Related Cytokines in a Mouse Model of HDM-Induced Allergic Asthma
title_sort	picroside ii attenuates airway inflammation by downregulating the transcription factor gata3 and th2-related cytokines in a mouse model of hdm-induced allergic asthma
description	Picroside II isolated from Pseudolysimachion rotundum var. subintegrum has been used as traditional medicine to treat inflammatory diseases. In this study, we assessed whether picroside II has inhibitory effects on airway inflammation in a mouse model of house dust mite (HDM)-induced asthma. In the HDM-induced asthmatic model, picroside II significantly reduced inflammatory cell counts in the bronchoalveolar lavage fluid (BALF), the levels of total immunoglobulin (Ig) E and HDM-specific IgE and IgG1 in serum, airway inflammation, and mucus hypersecretion in the lung tissues. ELISA analysis showed that picroside II down-regulated the levels of Th2-related cytokines (including IL-4, IL-5, and IL-13) and asthma-related mediators, but it up-regulated Th1-related cytokine, IFNγ in BALF. Picroside II also inhibited the expression of Th2 type cytokine genes and the transcription factor GATA3 in the lung tissues of HDM-induced mice. Finally, we demonstrated that picroside II significantly decreased the expression of GATA3 and Th2 cytokines in developing Th2 cells, consistent with in vivo results. Taken together, these results indicate that picroside II has protective effects on allergic asthma by reducing GATA3 expression and Th2 cytokine bias.
publisher	Public Library of Science
publishDate	2016
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5117775/
_version_	1613735563710431232

Picroside II Attenuates Airway Inflammation by Downregulating the Transcription Factor GATA3 and Th2-Related Cytokines in a Mouse Model of HDM-Induced Allergic Asthma

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