TP53 Gene Status Affects Survival in Advanced Mycosis Fungoides

TP53 is frequently mutated in different types of neoplasms including leukemia and lymphomas. Mutations of TP53 have also been reported in mycosis fungoides (MF), the most common type of cutaneous lymphoma. However, little is known about the frequency, spectrum of mutations, and their prognostic sign...

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Main Authors:	Wooler, Gitte, Melchior, Linea, Ralfkiaer, Elisabeth, Rahbek Gjerdrum, Lise Mette, Gniadecki, Robert
Format:	Online
Language:	English
Published:	Frontiers Media S.A. 2016
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5104736/

id	pubmed-5104736
recordtype	oai_dc
spelling	pubmed-51047362016-11-25 TP53 Gene Status Affects Survival in Advanced Mycosis Fungoides Wooler, Gitte Melchior, Linea Ralfkiaer, Elisabeth Rahbek Gjerdrum, Lise Mette Gniadecki, Robert Medicine TP53 is frequently mutated in different types of neoplasms including leukemia and lymphomas. Mutations of TP53 have also been reported in mycosis fungoides (MF), the most common type of cutaneous lymphoma. However, little is known about the frequency, spectrum of mutations, and their prognostic significance in MF. In this study, we have optimized the protocol for Sanger sequencing of TP53 using DNA extracted from archival paraffin-embedded biopsies. Of 19 samples from patients with stage IIB MF or higher, 31% harbored mutations in TP53. Overall survival of the patients with mutated TP53 was significantly shorter than median survival in the age- and stage-matched patients treated in our Institution. Distribution of mutations was heterogenous in TP53 exons; however, C > T transitions were common suggesting the causal role of ultraviolet radiation. We propose that TP53 mutation status would be useful for risk stratification of patients with advanced MF. Frontiers Media S.A. 2016-11-11 /pmc/articles/PMC5104736/ /pubmed/27891503 http://dx.doi.org/10.3389/fmed.2016.00051 Text en Copyright © 2016 Wooler, Melchior, Ralfkiaer, Rahbek Gjerdrum and Gniadecki. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Wooler, Gitte Melchior, Linea Ralfkiaer, Elisabeth Rahbek Gjerdrum, Lise Mette Gniadecki, Robert
spellingShingle	Wooler, Gitte Melchior, Linea Ralfkiaer, Elisabeth Rahbek Gjerdrum, Lise Mette Gniadecki, Robert TP53 Gene Status Affects Survival in Advanced Mycosis Fungoides
author_facet	Wooler, Gitte Melchior, Linea Ralfkiaer, Elisabeth Rahbek Gjerdrum, Lise Mette Gniadecki, Robert
author_sort	Wooler, Gitte
title	TP53 Gene Status Affects Survival in Advanced Mycosis Fungoides
title_short	TP53 Gene Status Affects Survival in Advanced Mycosis Fungoides
title_full	TP53 Gene Status Affects Survival in Advanced Mycosis Fungoides
title_fullStr	TP53 Gene Status Affects Survival in Advanced Mycosis Fungoides
title_full_unstemmed	TP53 Gene Status Affects Survival in Advanced Mycosis Fungoides
title_sort	tp53 gene status affects survival in advanced mycosis fungoides
description	TP53 is frequently mutated in different types of neoplasms including leukemia and lymphomas. Mutations of TP53 have also been reported in mycosis fungoides (MF), the most common type of cutaneous lymphoma. However, little is known about the frequency, spectrum of mutations, and their prognostic significance in MF. In this study, we have optimized the protocol for Sanger sequencing of TP53 using DNA extracted from archival paraffin-embedded biopsies. Of 19 samples from patients with stage IIB MF or higher, 31% harbored mutations in TP53. Overall survival of the patients with mutated TP53 was significantly shorter than median survival in the age- and stage-matched patients treated in our Institution. Distribution of mutations was heterogenous in TP53 exons; however, C > T transitions were common suggesting the causal role of ultraviolet radiation. We propose that TP53 mutation status would be useful for risk stratification of patients with advanced MF.
publisher	Frontiers Media S.A.
publishDate	2016
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5104736/
_version_	1613722261008678912

TP53 Gene Status Affects Survival in Advanced Mycosis Fungoides

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