NusG inhibits RNA polymerase backtracking by stabilizing the minimal transcription bubble

Universally conserved factors from NusG family bind at the upstream fork junction of transcription elongation complexes and modulate RNA synthesis in response to translation, processing, and folding of the nascent RNA. Escherichia coli NusG enhances transcription elongation in vitro by a poorly unde...

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Main Authors:	Turtola, Matti, Belogurov, Georgiy A
Format:	Online
Language:	English
Published:	eLife Sciences Publications, Ltd 2016
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5100998/

id	pubmed-5100998
recordtype	oai_dc
spelling	pubmed-51009982016-11-10 NusG inhibits RNA polymerase backtracking by stabilizing the minimal transcription bubble Turtola, Matti Belogurov, Georgiy A Biochemistry Universally conserved factors from NusG family bind at the upstream fork junction of transcription elongation complexes and modulate RNA synthesis in response to translation, processing, and folding of the nascent RNA. Escherichia coli NusG enhances transcription elongation in vitro by a poorly understood mechanism. Here we report that E. coli NusG slows Gre factor-stimulated cleavage of the nascent RNA, but does not measurably change the rates of single nucleotide addition and translocation by a non-paused RNA polymerase. We demonstrate that NusG slows RNA cleavage by inhibiting backtracking. This activity is abolished by mismatches in the upstream DNA and is independent of the gate and rudder loops, but is partially dependent on the lid loop. Our comprehensive mapping of the upstream fork junction by base analogue fluorescence and nucleic acids crosslinking suggests that NusG inhibits backtracking by stabilizing the minimal transcription bubble. eLife Sciences Publications, Ltd 2016-10-04 /pmc/articles/PMC5100998/ /pubmed/27697152 http://dx.doi.org/10.7554/eLife.18096 Text en © 2016, Turtola et al http://creativecommons.org/licenses/by/4.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Turtola, Matti Belogurov, Georgiy A
spellingShingle	Turtola, Matti Belogurov, Georgiy A NusG inhibits RNA polymerase backtracking by stabilizing the minimal transcription bubble
author_facet	Turtola, Matti Belogurov, Georgiy A
author_sort	Turtola, Matti
title	NusG inhibits RNA polymerase backtracking by stabilizing the minimal transcription bubble
title_short	NusG inhibits RNA polymerase backtracking by stabilizing the minimal transcription bubble
title_full	NusG inhibits RNA polymerase backtracking by stabilizing the minimal transcription bubble
title_fullStr	NusG inhibits RNA polymerase backtracking by stabilizing the minimal transcription bubble
title_full_unstemmed	NusG inhibits RNA polymerase backtracking by stabilizing the minimal transcription bubble
title_sort	nusg inhibits rna polymerase backtracking by stabilizing the minimal transcription bubble
description	Universally conserved factors from NusG family bind at the upstream fork junction of transcription elongation complexes and modulate RNA synthesis in response to translation, processing, and folding of the nascent RNA. Escherichia coli NusG enhances transcription elongation in vitro by a poorly understood mechanism. Here we report that E. coli NusG slows Gre factor-stimulated cleavage of the nascent RNA, but does not measurably change the rates of single nucleotide addition and translocation by a non-paused RNA polymerase. We demonstrate that NusG slows RNA cleavage by inhibiting backtracking. This activity is abolished by mismatches in the upstream DNA and is independent of the gate and rudder loops, but is partially dependent on the lid loop. Our comprehensive mapping of the upstream fork junction by base analogue fluorescence and nucleic acids crosslinking suggests that NusG inhibits backtracking by stabilizing the minimal transcription bubble.
publisher	eLife Sciences Publications, Ltd
publishDate	2016
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5100998/
_version_	1613718717514907648

NusG inhibits RNA polymerase backtracking by stabilizing the minimal transcription bubble

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