Random mutagenesis of the hyperthermophilic archaeon Pyrococcus furiosus using in vitro mariner transposition and natural transformation

Transposition mutagenesis is a powerful tool to identify the function of genes, reveal essential genes and generally to unravel the genetic basis of living organisms. However, transposon-mediated mutagenesis has only been successfully applied to a limited number of archaeal species and has never bee...

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Bibliographic Details
Main Authors: Guschinskaya, Natalia, Brunel, Romain, Tourte, Maxime, Lipscomb, Gina L., Adams, Michael W. W., Oger, Philippe, Charpentier, Xavier
Format: Online
Language:English
Published: Nature Publishing Group 2016
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5099854/