The Class II Trehalose 6-phosphate Synthase Gene PvTPS9 Modulates Trehalose Metabolism in Phaseolus vulgaris Nodules

The Class II Trehalose 6-phosphate Synthase Gene PvTPS9 Modulates Trehalose Metabolism in Phaseolus vulgaris Nodules

Legumes form symbioses with rhizobia, producing nitrogen-fixing nodules on the roots of the plant host. The network of plant signaling pathways affecting carbon metabolism may determine the final number of nodules. The trehalose biosynthetic pathway regulates carbon metabolism and plays a fundamenta...

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Main Authors: Barraza, Aarón, Contreras-Cubas, Cecilia, Estrada-Navarrete, Georgina, Reyes, José L., Juárez-Verdayes, Marco A., Avonce, Nelson, Quinto, Carmen, Díaz-Camino, Claudia, Sanchez, Federico
Format: Online
Language:English
Published: Frontiers Media S.A. 2016
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5088437/
id pubmed-5088437
recordtype oai_dc
spelling pubmed-50884372016-11-15 The Class II Trehalose 6-phosphate Synthase Gene PvTPS9 Modulates Trehalose Metabolism in Phaseolus vulgaris Nodules Barraza, Aarón Contreras-Cubas, Cecilia Estrada-Navarrete, Georgina Reyes, José L. Juárez-Verdayes, Marco A. Avonce, Nelson Quinto, Carmen Díaz-Camino, Claudia Sanchez, Federico Plant Science Legumes form symbioses with rhizobia, producing nitrogen-fixing nodules on the roots of the plant host. The network of plant signaling pathways affecting carbon metabolism may determine the final number of nodules. The trehalose biosynthetic pathway regulates carbon metabolism and plays a fundamental role in plant growth and development, as well as in plant-microbe interactions. The expression of genes for trehalose synthesis during nodule development suggests that this metabolite may play a role in legume-rhizobia symbiosis. In this work, PvTPS9, which encodes a Class II trehalose-6-phosphate synthase (TPS) of common bean (Phaseolus vulgaris), was silenced by RNA interference in transgenic nodules. The silencing of PvTPS9 in root nodules resulted in a reduction of 85% (± 1%) of its transcript, which correlated with a 30% decrease in trehalose contents of transgenic nodules and in untransformed leaves. Composite transgenic plants with PvTPS9 silenced in the roots showed no changes in nodule number and nitrogen fixation, but a severe reduction in plant biomass and altered transcript profiles of all Class II TPS genes. Our data suggest that PvTPS9 plays a key role in modulating trehalose metabolism in the symbiotic nodule and, therefore, in the whole plant. Frontiers Media S.A. 2016-11-01 /pmc/articles/PMC5088437/ /pubmed/27847509 http://dx.doi.org/10.3389/fpls.2016.01589 Text en Copyright © 2016 Barraza, Contreras-Cubas, Estrada-Navarrete, Reyes, Juárez-Verdayes, Avonce, Quinto, Díaz-Camino and Sanchez. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author Barraza, Aarón
Contreras-Cubas, Cecilia
Estrada-Navarrete, Georgina
Reyes, José L.
Juárez-Verdayes, Marco A.
Avonce, Nelson
Quinto, Carmen
Díaz-Camino, Claudia
Sanchez, Federico
spellingShingle Barraza, Aarón
Contreras-Cubas, Cecilia
Estrada-Navarrete, Georgina
Reyes, José L.
Juárez-Verdayes, Marco A.
Avonce, Nelson
Quinto, Carmen
Díaz-Camino, Claudia
Sanchez, Federico
The Class II Trehalose 6-phosphate Synthase Gene PvTPS9 Modulates Trehalose Metabolism in Phaseolus vulgaris Nodules
author_facet Barraza, Aarón
Contreras-Cubas, Cecilia
Estrada-Navarrete, Georgina
Reyes, José L.
Juárez-Verdayes, Marco A.
Avonce, Nelson
Quinto, Carmen
Díaz-Camino, Claudia
Sanchez, Federico
author_sort Barraza, Aarón
title The Class II Trehalose 6-phosphate Synthase Gene PvTPS9 Modulates Trehalose Metabolism in Phaseolus vulgaris Nodules
title_short The Class II Trehalose 6-phosphate Synthase Gene PvTPS9 Modulates Trehalose Metabolism in Phaseolus vulgaris Nodules
title_full The Class II Trehalose 6-phosphate Synthase Gene PvTPS9 Modulates Trehalose Metabolism in Phaseolus vulgaris Nodules
title_fullStr The Class II Trehalose 6-phosphate Synthase Gene PvTPS9 Modulates Trehalose Metabolism in Phaseolus vulgaris Nodules
title_full_unstemmed The Class II Trehalose 6-phosphate Synthase Gene PvTPS9 Modulates Trehalose Metabolism in Phaseolus vulgaris Nodules
title_sort class ii trehalose 6-phosphate synthase gene pvtps9 modulates trehalose metabolism in phaseolus vulgaris nodules
description Legumes form symbioses with rhizobia, producing nitrogen-fixing nodules on the roots of the plant host. The network of plant signaling pathways affecting carbon metabolism may determine the final number of nodules. The trehalose biosynthetic pathway regulates carbon metabolism and plays a fundamental role in plant growth and development, as well as in plant-microbe interactions. The expression of genes for trehalose synthesis during nodule development suggests that this metabolite may play a role in legume-rhizobia symbiosis. In this work, PvTPS9, which encodes a Class II trehalose-6-phosphate synthase (TPS) of common bean (Phaseolus vulgaris), was silenced by RNA interference in transgenic nodules. The silencing of PvTPS9 in root nodules resulted in a reduction of 85% (± 1%) of its transcript, which correlated with a 30% decrease in trehalose contents of transgenic nodules and in untransformed leaves. Composite transgenic plants with PvTPS9 silenced in the roots showed no changes in nodule number and nitrogen fixation, but a severe reduction in plant biomass and altered transcript profiles of all Class II TPS genes. Our data suggest that PvTPS9 plays a key role in modulating trehalose metabolism in the symbiotic nodule and, therefore, in the whole plant.
publisher Frontiers Media S.A.
publishDate 2016
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5088437/
_version_ 1613706099310985216

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