The Biofilm Lifestyle Involves an Increase in Bacterial Membrane Saturated Fatty Acids

Biofilm formation on contact surfaces contributes to persistence of foodborne pathogens all along the food and feed chain. The specific physiological features of bacterial cells embedded in biofilms contribute to their high tolerance to environmental stresses, including the action of antimicrobial c...

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Main Authors:	Dubois-Brissonnet, Florence, Trotier, Elsa, Briandet, Romain
Format:	Online
Language:	English
Published:	Frontiers Media S.A. 2016
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5083788/

id	pubmed-5083788
recordtype	oai_dc
spelling	pubmed-50837882016-11-11 The Biofilm Lifestyle Involves an Increase in Bacterial Membrane Saturated Fatty Acids Dubois-Brissonnet, Florence Trotier, Elsa Briandet, Romain Microbiology Biofilm formation on contact surfaces contributes to persistence of foodborne pathogens all along the food and feed chain. The specific physiological features of bacterial cells embedded in biofilms contribute to their high tolerance to environmental stresses, including the action of antimicrobial compounds. As membrane lipid adaptation is a vital facet of bacterial response when cells are submitted to harsh or unstable conditions, we focused here on membrane fatty acid composition of biofilm cells as compared to their free-growing counterparts. Pathogenic bacteria (Staphylococcus aureus, Listeria monocytogenes, Pseudomonas aeruginosa, Salmonella Typhimurium) were cultivated in planktonic or biofilm states and membrane fatty acid analyses were performed on whole cells in both conditions. The percentage of saturated fatty acids increases in biofilm cells in all cases, with a concomitant decrease of branched-chain fatty acids for Gram-positive bacteria, or with a decrease in the sum of other fatty acids for Gram-negative bacteria. We propose that increased membrane saturation in biofilm cells is an adaptive stress response that allows bacteria to limit exchanges, save energy, and survive. Reprogramming of membrane fluidity in biofilm cells might explain specific biofilm behavior including bacterial recalcitrance to biocide action. Frontiers Media S.A. 2016-10-28 /pmc/articles/PMC5083788/ /pubmed/27840623 http://dx.doi.org/10.3389/fmicb.2016.01673 Text en Copyright © 2016 Dubois-Brissonnet, Trotier and Briandet. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Dubois-Brissonnet, Florence Trotier, Elsa Briandet, Romain
spellingShingle	Dubois-Brissonnet, Florence Trotier, Elsa Briandet, Romain The Biofilm Lifestyle Involves an Increase in Bacterial Membrane Saturated Fatty Acids
author_facet	Dubois-Brissonnet, Florence Trotier, Elsa Briandet, Romain
author_sort	Dubois-Brissonnet, Florence
title	The Biofilm Lifestyle Involves an Increase in Bacterial Membrane Saturated Fatty Acids
title_short	The Biofilm Lifestyle Involves an Increase in Bacterial Membrane Saturated Fatty Acids
title_full	The Biofilm Lifestyle Involves an Increase in Bacterial Membrane Saturated Fatty Acids
title_fullStr	The Biofilm Lifestyle Involves an Increase in Bacterial Membrane Saturated Fatty Acids
title_full_unstemmed	The Biofilm Lifestyle Involves an Increase in Bacterial Membrane Saturated Fatty Acids
title_sort	biofilm lifestyle involves an increase in bacterial membrane saturated fatty acids
description	Biofilm formation on contact surfaces contributes to persistence of foodborne pathogens all along the food and feed chain. The specific physiological features of bacterial cells embedded in biofilms contribute to their high tolerance to environmental stresses, including the action of antimicrobial compounds. As membrane lipid adaptation is a vital facet of bacterial response when cells are submitted to harsh or unstable conditions, we focused here on membrane fatty acid composition of biofilm cells as compared to their free-growing counterparts. Pathogenic bacteria (Staphylococcus aureus, Listeria monocytogenes, Pseudomonas aeruginosa, Salmonella Typhimurium) were cultivated in planktonic or biofilm states and membrane fatty acid analyses were performed on whole cells in both conditions. The percentage of saturated fatty acids increases in biofilm cells in all cases, with a concomitant decrease of branched-chain fatty acids for Gram-positive bacteria, or with a decrease in the sum of other fatty acids for Gram-negative bacteria. We propose that increased membrane saturation in biofilm cells is an adaptive stress response that allows bacteria to limit exchanges, save energy, and survive. Reprogramming of membrane fluidity in biofilm cells might explain specific biofilm behavior including bacterial recalcitrance to biocide action.
publisher	Frontiers Media S.A.
publishDate	2016
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5083788/
_version_	1613701556835713024

The Biofilm Lifestyle Involves an Increase in Bacterial Membrane Saturated Fatty Acids

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