Characterization of the Verticillium dahliae Exoproteome Involves in Pathogenicity from Cotton-Containing Medium

Verticillium wilt, caused by the Verticillium dahliae phytopathogen, is a devastating disease affecting many economically important crops. Previous studies have shown that the exoproteome of V. dahliae plays a significant role in this pathogenic process, but the components and mechanisms that underl...

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Main Authors:	Chen, Jie-Yin, Xiao, Hong-Li, Gui, Yue-Jing, Zhang, Dan-Dan, Li, Lei, Bao, Yu-Ming, Dai, Xiao-Feng
Format:	Online
Language:	English
Published:	Frontiers Media S.A. 2016
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5083787/

id	pubmed-5083787
recordtype	oai_dc
spelling	pubmed-50837872016-11-11 Characterization of the Verticillium dahliae Exoproteome Involves in Pathogenicity from Cotton-Containing Medium Chen, Jie-Yin Xiao, Hong-Li Gui, Yue-Jing Zhang, Dan-Dan Li, Lei Bao, Yu-Ming Dai, Xiao-Feng Microbiology Verticillium wilt, caused by the Verticillium dahliae phytopathogen, is a devastating disease affecting many economically important crops. Previous studies have shown that the exoproteome of V. dahliae plays a significant role in this pathogenic process, but the components and mechanisms that underlie this remain unclear. In this study, the exoproteome of V. dahliae was induced in a cotton-containing C’zapek-Dox (CCD) medium and quantified using the high-throughput isobaric tag technique for relative and absolute quantification (iTRAQ). Results showed that the abundance of 271 secreted proteins was affected by the CCD medium, of which 172 contain typical signal peptides generally produced by the Golgi/endoplasmic reticulum (ER). These enhanced abundance proteins were predominantly enriched in carbohydrate hydrolases; 126 were classified as carbohydrate-active (CAZymes) and almost all were significantly up-regulated in the CCD medium. Results showed that CAZymes proteins 30 and 22 participate in pectin and cellulose degradation pathways, corresponding with the transcription levels of several genes encoded plant cell wall degradation enzyme activated significantly during cotton infection. In addition, targeted deletion of two pectin lyase genes (VdPL3.1 and VdPL3.3) impaired wilt virulence to cotton. This study demonstrates that the V. dahliae exoproteome plays a crucial role in the development of symptoms of wilting and necrosis, predominantly via the pathogenic mechanisms of plant cell wall degradation as part of host plant infection. Frontiers Media S.A. 2016-10-28 /pmc/articles/PMC5083787/ /pubmed/27840627 http://dx.doi.org/10.3389/fmicb.2016.01709 Text en Copyright © 2016 Chen, Xiao, Gui, Zhang, Li, Bao and Dai. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Chen, Jie-Yin Xiao, Hong-Li Gui, Yue-Jing Zhang, Dan-Dan Li, Lei Bao, Yu-Ming Dai, Xiao-Feng
spellingShingle	Chen, Jie-Yin Xiao, Hong-Li Gui, Yue-Jing Zhang, Dan-Dan Li, Lei Bao, Yu-Ming Dai, Xiao-Feng Characterization of the Verticillium dahliae Exoproteome Involves in Pathogenicity from Cotton-Containing Medium
author_facet	Chen, Jie-Yin Xiao, Hong-Li Gui, Yue-Jing Zhang, Dan-Dan Li, Lei Bao, Yu-Ming Dai, Xiao-Feng
author_sort	Chen, Jie-Yin
title	Characterization of the Verticillium dahliae Exoproteome Involves in Pathogenicity from Cotton-Containing Medium
title_short	Characterization of the Verticillium dahliae Exoproteome Involves in Pathogenicity from Cotton-Containing Medium
title_full	Characterization of the Verticillium dahliae Exoproteome Involves in Pathogenicity from Cotton-Containing Medium
title_fullStr	Characterization of the Verticillium dahliae Exoproteome Involves in Pathogenicity from Cotton-Containing Medium
title_full_unstemmed	Characterization of the Verticillium dahliae Exoproteome Involves in Pathogenicity from Cotton-Containing Medium
title_sort	characterization of the verticillium dahliae exoproteome involves in pathogenicity from cotton-containing medium
description	Verticillium wilt, caused by the Verticillium dahliae phytopathogen, is a devastating disease affecting many economically important crops. Previous studies have shown that the exoproteome of V. dahliae plays a significant role in this pathogenic process, but the components and mechanisms that underlie this remain unclear. In this study, the exoproteome of V. dahliae was induced in a cotton-containing C’zapek-Dox (CCD) medium and quantified using the high-throughput isobaric tag technique for relative and absolute quantification (iTRAQ). Results showed that the abundance of 271 secreted proteins was affected by the CCD medium, of which 172 contain typical signal peptides generally produced by the Golgi/endoplasmic reticulum (ER). These enhanced abundance proteins were predominantly enriched in carbohydrate hydrolases; 126 were classified as carbohydrate-active (CAZymes) and almost all were significantly up-regulated in the CCD medium. Results showed that CAZymes proteins 30 and 22 participate in pectin and cellulose degradation pathways, corresponding with the transcription levels of several genes encoded plant cell wall degradation enzyme activated significantly during cotton infection. In addition, targeted deletion of two pectin lyase genes (VdPL3.1 and VdPL3.3) impaired wilt virulence to cotton. This study demonstrates that the V. dahliae exoproteome plays a crucial role in the development of symptoms of wilting and necrosis, predominantly via the pathogenic mechanisms of plant cell wall degradation as part of host plant infection.
publisher	Frontiers Media S.A.
publishDate	2016
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5083787/
_version_	1613701555639287808

Characterization of the Verticillium dahliae Exoproteome Involves in Pathogenicity from Cotton-Containing Medium

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