Microinjection-based generation of mutant mice with a double mutation and a 0.5 Mb deletion in their genome by the CRISPR/Cas9 system

Microinjection-based generation of mutant mice with a double mutation and a 0.5 Mb deletion in their genome by the CRISPR/Cas9 system

The clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) system is a useful tool for genome editing. In this study, using a microinjection-based CRISPR/Cas9 system, we efficiently generated mouse lines carrying mutations at the Irx3 and Irx5 loci, whi...

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Bibliographic Details
Main Authors: HARA, Satoshi, KATO, Tomoko, GOTO, Yuji, KUBOTA, Souichirou, TAMANO, Moe, TERAO, Miho, TAKADA, Shuji
Format: Online
Language:English
Published: The Society for Reproduction and Development 2016
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5081742/

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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5081742/

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