miR-200 family controls late steps of postnatal forebrain neurogenesis via Zeb2 inhibition

During neurogenesis, generation, migration and integration of the correct numbers of each neuron sub-type depends on complex molecular interactions in space and time. MicroRNAs represent a key control level allowing the flexibility and stability needed for this process. Insight into the role of this...

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Main Authors:	Beclin, Christophe, Follert, Philipp, Stappers, Elke, Barral, Serena, Nathalie, Coré, de Chevigny, Antoine, Magnone, Virginie, Lebrigand, Kévin, Bissels, Ute, Huylebroeck, Danny, Bosio, Andreas, Barbry, Pascal, Seuntjens, Eve, Cremer, Harold
Format:	Online
Language:	English
Published:	Nature Publishing Group 2016
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5073329/

id	pubmed-5073329
recordtype	oai_dc
spelling	pubmed-50733292016-10-26 miR-200 family controls late steps of postnatal forebrain neurogenesis via Zeb2 inhibition Beclin, Christophe Follert, Philipp Stappers, Elke Barral, Serena Nathalie, Coré de Chevigny, Antoine Magnone, Virginie Lebrigand, Kévin Bissels, Ute Huylebroeck, Danny Bosio, Andreas Barbry, Pascal Seuntjens, Eve Cremer, Harold Article During neurogenesis, generation, migration and integration of the correct numbers of each neuron sub-type depends on complex molecular interactions in space and time. MicroRNAs represent a key control level allowing the flexibility and stability needed for this process. Insight into the role of this regulatory pathway in the brain is still limited. We performed a sequential experimental approach using postnatal olfactory bulb neurogenesis in mice, starting from global expression analyses to the investigation of functional interactions between defined microRNAs and their targets. Deep sequencing of small RNAs extracted from defined compartments of the postnatal neurogenic system demonstrated that the miR-200 family is specifically induced during late neuronal differentiation stages. Using in vivo strategies we interfered with the entire miR-200 family in loss- and gain-of-function settings, showing a role of miR-200 in neuronal maturation. This function is mediated by targeting the transcription factor Zeb2. Interestingly, so far functional interaction between miR-200 and Zeb2 has been exclusively reported in cancer or cultured stem cells. Our data demonstrate that this regulatory interaction is also active during normal neurogenesis. Nature Publishing Group 2016-10-21 /pmc/articles/PMC5073329/ /pubmed/27767083 http://dx.doi.org/10.1038/srep35729 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Beclin, Christophe Follert, Philipp Stappers, Elke Barral, Serena Nathalie, Coré de Chevigny, Antoine Magnone, Virginie Lebrigand, Kévin Bissels, Ute Huylebroeck, Danny Bosio, Andreas Barbry, Pascal Seuntjens, Eve Cremer, Harold
spellingShingle	Beclin, Christophe Follert, Philipp Stappers, Elke Barral, Serena Nathalie, Coré de Chevigny, Antoine Magnone, Virginie Lebrigand, Kévin Bissels, Ute Huylebroeck, Danny Bosio, Andreas Barbry, Pascal Seuntjens, Eve Cremer, Harold miR-200 family controls late steps of postnatal forebrain neurogenesis via Zeb2 inhibition
author_facet	Beclin, Christophe Follert, Philipp Stappers, Elke Barral, Serena Nathalie, Coré de Chevigny, Antoine Magnone, Virginie Lebrigand, Kévin Bissels, Ute Huylebroeck, Danny Bosio, Andreas Barbry, Pascal Seuntjens, Eve Cremer, Harold
author_sort	Beclin, Christophe
title	miR-200 family controls late steps of postnatal forebrain neurogenesis via Zeb2 inhibition
title_short	miR-200 family controls late steps of postnatal forebrain neurogenesis via Zeb2 inhibition
title_full	miR-200 family controls late steps of postnatal forebrain neurogenesis via Zeb2 inhibition
title_fullStr	miR-200 family controls late steps of postnatal forebrain neurogenesis via Zeb2 inhibition
title_full_unstemmed	miR-200 family controls late steps of postnatal forebrain neurogenesis via Zeb2 inhibition
title_sort	mir-200 family controls late steps of postnatal forebrain neurogenesis via zeb2 inhibition
description	During neurogenesis, generation, migration and integration of the correct numbers of each neuron sub-type depends on complex molecular interactions in space and time. MicroRNAs represent a key control level allowing the flexibility and stability needed for this process. Insight into the role of this regulatory pathway in the brain is still limited. We performed a sequential experimental approach using postnatal olfactory bulb neurogenesis in mice, starting from global expression analyses to the investigation of functional interactions between defined microRNAs and their targets. Deep sequencing of small RNAs extracted from defined compartments of the postnatal neurogenic system demonstrated that the miR-200 family is specifically induced during late neuronal differentiation stages. Using in vivo strategies we interfered with the entire miR-200 family in loss- and gain-of-function settings, showing a role of miR-200 in neuronal maturation. This function is mediated by targeting the transcription factor Zeb2. Interestingly, so far functional interaction between miR-200 and Zeb2 has been exclusively reported in cancer or cultured stem cells. Our data demonstrate that this regulatory interaction is also active during normal neurogenesis.
publisher	Nature Publishing Group
publishDate	2016
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5073329/
_version_	1613691946826465280

miR-200 family controls late steps of postnatal forebrain neurogenesis via Zeb2 inhibition

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