Identification of amino acids related to catalytic function of Sulfolobus solfataricus P1 carboxylesterase by site-directed mutagenesis and molecular modeling
The archaeon Sulfolobus solfataricus P1 carboxylesterase is a thermostable enzyme with a molecular mass of 33.5 kDa belonging to the mammalian hormone-sensitive lipase (HSL) family. In our previous study, we purified the enzyme and suggested the expected amino acids related to its catalysis by chemi...
| Main Authors: | , , , , |
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| Format: | Online |
| Language: | English |
| Published: |
Korean Society for Biochemistry and Molecular Biology
2016
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5070724/ |