Subcellular Evidence for Biogenesis of Autophagosomal Membrane during Spermiogenesis In vivo

Although autophagosome formation has attracted substantial attention, the origin and the source of the autophagosomal membrane remains unresolved. The present study was designed to investigate in vivo subcellular evidence for the biogenesis of autophagosomal membrane during spermiogenesis using tran...

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Main Authors: Huang, Yufei, Yang, Ping, Liu, Tengfei, Chen, Hong, Chu, Xiaoya, Ahmad, Nisar, Zhang, Qian, Li, Quanfu, Hu, Lisi, Liu, Yi, Chen, Qiusheng
Format: Online
Language:English
Published: Frontiers Media S.A. 2016
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5067523/
id pubmed-5067523
recordtype oai_dc
spelling pubmed-50675232016-11-01 Subcellular Evidence for Biogenesis of Autophagosomal Membrane during Spermiogenesis In vivo Huang, Yufei Yang, Ping Liu, Tengfei Chen, Hong Chu, Xiaoya Ahmad, Nisar Zhang, Qian Li, Quanfu Hu, Lisi Liu, Yi Chen, Qiusheng Physiology Although autophagosome formation has attracted substantial attention, the origin and the source of the autophagosomal membrane remains unresolved. The present study was designed to investigate in vivo subcellular evidence for the biogenesis of autophagosomal membrane during spermiogenesis using transmission-electron microscopy (TEM), Western blots and immunohistochemistry in samples from the Chinese soft-shelled turtle. The testis expressed LC3-II protein, which was located within spermatids at different stages of differentiation and indicated active autophagy. TEM showed that numerous autophagosomes were developed inside spermatids. Many endoplasmic reticulum (ER) were transferred into a special “Chrysanthemum flower center” (CFC) in which several double-layer isolation membranes (IM) were formed and extended. The elongated IM always engulfed some cytoplasm and various structures. Narrow tubules connected the ends of multiple ER and the CFC. The CFC was more developed in spermatids with compact nuclei than in spermatids with granular nuclei. An IM could also be transformed from a single ER. Sometimes an IM extended from a trans-Golgi network and wrapped different structures. The plasma membrane of the spermatid invaginated to form vesicles that were distributed among various endosomes around the CFC during spermiogenesis. All this cellular evidence suggests that, in vivo, IM was developed mainly by CFC produced from ER within differentiating spermatids during spermiogenesis. Vesicles from Golgi complexes, plasma membranes and endosomes might also be the sources of the autophagosome membrane. Frontiers Media S.A. 2016-10-18 /pmc/articles/PMC5067523/ /pubmed/27803675 http://dx.doi.org/10.3389/fphys.2016.00470 Text en Copyright © 2016 Huang, Yang, Liu, Chen, Chu, Ahmad, Zhang, Li, Hu, Liu and Chen. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author Huang, Yufei
Yang, Ping
Liu, Tengfei
Chen, Hong
Chu, Xiaoya
Ahmad, Nisar
Zhang, Qian
Li, Quanfu
Hu, Lisi
Liu, Yi
Chen, Qiusheng
spellingShingle Huang, Yufei
Yang, Ping
Liu, Tengfei
Chen, Hong
Chu, Xiaoya
Ahmad, Nisar
Zhang, Qian
Li, Quanfu
Hu, Lisi
Liu, Yi
Chen, Qiusheng
Subcellular Evidence for Biogenesis of Autophagosomal Membrane during Spermiogenesis In vivo
author_facet Huang, Yufei
Yang, Ping
Liu, Tengfei
Chen, Hong
Chu, Xiaoya
Ahmad, Nisar
Zhang, Qian
Li, Quanfu
Hu, Lisi
Liu, Yi
Chen, Qiusheng
author_sort Huang, Yufei
title Subcellular Evidence for Biogenesis of Autophagosomal Membrane during Spermiogenesis In vivo
title_short Subcellular Evidence for Biogenesis of Autophagosomal Membrane during Spermiogenesis In vivo
title_full Subcellular Evidence for Biogenesis of Autophagosomal Membrane during Spermiogenesis In vivo
title_fullStr Subcellular Evidence for Biogenesis of Autophagosomal Membrane during Spermiogenesis In vivo
title_full_unstemmed Subcellular Evidence for Biogenesis of Autophagosomal Membrane during Spermiogenesis In vivo
title_sort subcellular evidence for biogenesis of autophagosomal membrane during spermiogenesis in vivo
description Although autophagosome formation has attracted substantial attention, the origin and the source of the autophagosomal membrane remains unresolved. The present study was designed to investigate in vivo subcellular evidence for the biogenesis of autophagosomal membrane during spermiogenesis using transmission-electron microscopy (TEM), Western blots and immunohistochemistry in samples from the Chinese soft-shelled turtle. The testis expressed LC3-II protein, which was located within spermatids at different stages of differentiation and indicated active autophagy. TEM showed that numerous autophagosomes were developed inside spermatids. Many endoplasmic reticulum (ER) were transferred into a special “Chrysanthemum flower center” (CFC) in which several double-layer isolation membranes (IM) were formed and extended. The elongated IM always engulfed some cytoplasm and various structures. Narrow tubules connected the ends of multiple ER and the CFC. The CFC was more developed in spermatids with compact nuclei than in spermatids with granular nuclei. An IM could also be transformed from a single ER. Sometimes an IM extended from a trans-Golgi network and wrapped different structures. The plasma membrane of the spermatid invaginated to form vesicles that were distributed among various endosomes around the CFC during spermiogenesis. All this cellular evidence suggests that, in vivo, IM was developed mainly by CFC produced from ER within differentiating spermatids during spermiogenesis. Vesicles from Golgi complexes, plasma membranes and endosomes might also be the sources of the autophagosome membrane.
publisher Frontiers Media S.A.
publishDate 2016
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5067523/
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