Identification of Novel Rosavirus Species That Infects Diverse Rodent Species and Causes Multisystemic Dissemination in Mouse Model

While novel picornaviruses are being discovered in rodents, their host range and pathogenicity are largely unknown. We identified two novel picornaviruses, rosavirus B from the street rat, Norway rat, and rosavirus C from five different wild rat species (chestnut spiny rat, greater bandicoot rat, In...

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Main Authors:	Lau, Susanna K. P., Woo, Patrick C. Y., Li, Kenneth S. M., Zhang, Hao-Ji, Fan, Rachel Y. Y., Zhang, Anna J. X., Chan, Brandon C. C., Lam, Carol S. F., Yip, Cyril C. Y., Yuen, Ming-Chi, Chan, Kwok-Hung, Chen, Zhi-Wei, Yuen, Kwok-Yung
Format:	Online
Language:	English
Published:	Public Library of Science 2016
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5063349/

id	pubmed-5063349
recordtype	oai_dc
spelling	pubmed-50633492016-11-04 Identification of Novel Rosavirus Species That Infects Diverse Rodent Species and Causes Multisystemic Dissemination in Mouse Model Lau, Susanna K. P. Woo, Patrick C. Y. Li, Kenneth S. M. Zhang, Hao-Ji Fan, Rachel Y. Y. Zhang, Anna J. X. Chan, Brandon C. C. Lam, Carol S. F. Yip, Cyril C. Y. Yuen, Ming-Chi Chan, Kwok-Hung Chen, Zhi-Wei Yuen, Kwok-Yung Research Article While novel picornaviruses are being discovered in rodents, their host range and pathogenicity are largely unknown. We identified two novel picornaviruses, rosavirus B from the street rat, Norway rat, and rosavirus C from five different wild rat species (chestnut spiny rat, greater bandicoot rat, Indochinese forest rat, roof rat and Coxing's white-bellied rat) in China. Analysis of 13 complete genome sequences showed that “Rosavirus B” and “Rosavirus C” represent two potentially novel picornavirus species infecting different rodents. Though being most closely related to rosavirus A, rosavirus B and C possessed distinct protease cleavage sites and variations in Yn-Xm-AUG sequence in 5’UTR and myristylation site in VP4. Anti-rosavirus B VP1 antibodies were detected in Norway rats, whereas anti-rosavirus C VP1 and neutralizing antibodies were detected in Indochinese forest rats and Coxing's white-bellied rats. While the highest prevalence was observed in Coxing's white-bellied rats by RT-PCR, the detection of rosavirus C from different rat species suggests potential interspecies transmission. Rosavirus C isolated from 3T3 cells causes multisystemic diseases in a mouse model, with high viral loads and positive viral antigen expression in organs of infected mice after oral or intracerebral inoculation. Histological examination revealed alveolar fluid exudation, interstitial infiltration, alveolar fluid exudate and wall thickening in lungs, and hepatocyte degeneration and lymphocytic/monocytic inflammatory infiltrates with giant cell formation in liver sections of sacrificed mice. Since rosavirus A2 has been detected in fecal samples of children, further studies should elucidate the pathogenicity and emergence potential of different rosaviruses. Public Library of Science 2016-10-13 /pmc/articles/PMC5063349/ /pubmed/27737017 http://dx.doi.org/10.1371/journal.ppat.1005911 Text en © 2016 Lau et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Lau, Susanna K. P. Woo, Patrick C. Y. Li, Kenneth S. M. Zhang, Hao-Ji Fan, Rachel Y. Y. Zhang, Anna J. X. Chan, Brandon C. C. Lam, Carol S. F. Yip, Cyril C. Y. Yuen, Ming-Chi Chan, Kwok-Hung Chen, Zhi-Wei Yuen, Kwok-Yung
spellingShingle	Lau, Susanna K. P. Woo, Patrick C. Y. Li, Kenneth S. M. Zhang, Hao-Ji Fan, Rachel Y. Y. Zhang, Anna J. X. Chan, Brandon C. C. Lam, Carol S. F. Yip, Cyril C. Y. Yuen, Ming-Chi Chan, Kwok-Hung Chen, Zhi-Wei Yuen, Kwok-Yung Identification of Novel Rosavirus Species That Infects Diverse Rodent Species and Causes Multisystemic Dissemination in Mouse Model
author_facet	Lau, Susanna K. P. Woo, Patrick C. Y. Li, Kenneth S. M. Zhang, Hao-Ji Fan, Rachel Y. Y. Zhang, Anna J. X. Chan, Brandon C. C. Lam, Carol S. F. Yip, Cyril C. Y. Yuen, Ming-Chi Chan, Kwok-Hung Chen, Zhi-Wei Yuen, Kwok-Yung
author_sort	Lau, Susanna K. P.
title	Identification of Novel Rosavirus Species That Infects Diverse Rodent Species and Causes Multisystemic Dissemination in Mouse Model
title_short	Identification of Novel Rosavirus Species That Infects Diverse Rodent Species and Causes Multisystemic Dissemination in Mouse Model
title_full	Identification of Novel Rosavirus Species That Infects Diverse Rodent Species and Causes Multisystemic Dissemination in Mouse Model
title_fullStr	Identification of Novel Rosavirus Species That Infects Diverse Rodent Species and Causes Multisystemic Dissemination in Mouse Model
title_full_unstemmed	Identification of Novel Rosavirus Species That Infects Diverse Rodent Species and Causes Multisystemic Dissemination in Mouse Model
title_sort	identification of novel rosavirus species that infects diverse rodent species and causes multisystemic dissemination in mouse model
description	While novel picornaviruses are being discovered in rodents, their host range and pathogenicity are largely unknown. We identified two novel picornaviruses, rosavirus B from the street rat, Norway rat, and rosavirus C from five different wild rat species (chestnut spiny rat, greater bandicoot rat, Indochinese forest rat, roof rat and Coxing's white-bellied rat) in China. Analysis of 13 complete genome sequences showed that “Rosavirus B” and “Rosavirus C” represent two potentially novel picornavirus species infecting different rodents. Though being most closely related to rosavirus A, rosavirus B and C possessed distinct protease cleavage sites and variations in Yn-Xm-AUG sequence in 5’UTR and myristylation site in VP4. Anti-rosavirus B VP1 antibodies were detected in Norway rats, whereas anti-rosavirus C VP1 and neutralizing antibodies were detected in Indochinese forest rats and Coxing's white-bellied rats. While the highest prevalence was observed in Coxing's white-bellied rats by RT-PCR, the detection of rosavirus C from different rat species suggests potential interspecies transmission. Rosavirus C isolated from 3T3 cells causes multisystemic diseases in a mouse model, with high viral loads and positive viral antigen expression in organs of infected mice after oral or intracerebral inoculation. Histological examination revealed alveolar fluid exudation, interstitial infiltration, alveolar fluid exudate and wall thickening in lungs, and hepatocyte degeneration and lymphocytic/monocytic inflammatory infiltrates with giant cell formation in liver sections of sacrificed mice. Since rosavirus A2 has been detected in fecal samples of children, further studies should elucidate the pathogenicity and emergence potential of different rosaviruses.
publisher	Public Library of Science
publishDate	2016
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5063349/
_version_	1613683515303395328

Identification of Novel Rosavirus Species That Infects Diverse Rodent Species and Causes Multisystemic Dissemination in Mouse Model

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