Genomic evidence for plant-parasitic nematodes as the earliest Wolbachia hosts

Wolbachia, one of the most widespread endosymbionts, is a target for biological control of mosquito-borne diseases (malaria and dengue virus), and antibiotic elimination of infectious filarial nematodes. We sequenced and analyzed the genome of a new Wolbachia strain (wPpe) in the plant-parasitic nem...

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Main Authors:	Brown, Amanda M. V., Wasala, Sulochana K., Howe, Dana K., Peetz, Amy B., Zasada, Inga A., Denver, Dee R.
Format:	Online
Language:	English
Published:	Nature Publishing Group 2016
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5062116/

id	pubmed-5062116
recordtype	oai_dc
spelling	pubmed-50621162016-10-24 Genomic evidence for plant-parasitic nematodes as the earliest Wolbachia hosts Brown, Amanda M. V. Wasala, Sulochana K. Howe, Dana K. Peetz, Amy B. Zasada, Inga A. Denver, Dee R. Article Wolbachia, one of the most widespread endosymbionts, is a target for biological control of mosquito-borne diseases (malaria and dengue virus), and antibiotic elimination of infectious filarial nematodes. We sequenced and analyzed the genome of a new Wolbachia strain (wPpe) in the plant-parasitic nematode Pratylenchus penetrans. Phylogenomic analyses placed wPpe as the earliest diverging Wolbachia, suggesting two evolutionary invasions into nematodes. The next branches comprised strains in sap-feeding insects, suggesting Wolbachia may have first evolved as a nutritional mutualist. Genome size, protein content, %GC, and repetitive DNA allied wPpe with mutualistic Wolbachia, whereas gene repertoire analyses placed it between parasite (A, B) and mutualist (C, D, F) groups. Conservation of iron metabolism genes across Wolbachia suggests iron homeostasis as a potential factor in its success. This study enhances our understanding of this globally pandemic endosymbiont, highlighting genetic patterns associated with host changes. Combined with future work on this strain, these genomic data could help provide potential new targets for plant-parasitic nematode control. Nature Publishing Group 2016-10-13 /pmc/articles/PMC5062116/ /pubmed/27734894 http://dx.doi.org/10.1038/srep34955 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Brown, Amanda M. V. Wasala, Sulochana K. Howe, Dana K. Peetz, Amy B. Zasada, Inga A. Denver, Dee R.
spellingShingle	Brown, Amanda M. V. Wasala, Sulochana K. Howe, Dana K. Peetz, Amy B. Zasada, Inga A. Denver, Dee R. Genomic evidence for plant-parasitic nematodes as the earliest Wolbachia hosts
author_facet	Brown, Amanda M. V. Wasala, Sulochana K. Howe, Dana K. Peetz, Amy B. Zasada, Inga A. Denver, Dee R.
author_sort	Brown, Amanda M. V.
title	Genomic evidence for plant-parasitic nematodes as the earliest Wolbachia hosts
title_short	Genomic evidence for plant-parasitic nematodes as the earliest Wolbachia hosts
title_full	Genomic evidence for plant-parasitic nematodes as the earliest Wolbachia hosts
title_fullStr	Genomic evidence for plant-parasitic nematodes as the earliest Wolbachia hosts
title_full_unstemmed	Genomic evidence for plant-parasitic nematodes as the earliest Wolbachia hosts
title_sort	genomic evidence for plant-parasitic nematodes as the earliest wolbachia hosts
description	Wolbachia, one of the most widespread endosymbionts, is a target for biological control of mosquito-borne diseases (malaria and dengue virus), and antibiotic elimination of infectious filarial nematodes. We sequenced and analyzed the genome of a new Wolbachia strain (wPpe) in the plant-parasitic nematode Pratylenchus penetrans. Phylogenomic analyses placed wPpe as the earliest diverging Wolbachia, suggesting two evolutionary invasions into nematodes. The next branches comprised strains in sap-feeding insects, suggesting Wolbachia may have first evolved as a nutritional mutualist. Genome size, protein content, %GC, and repetitive DNA allied wPpe with mutualistic Wolbachia, whereas gene repertoire analyses placed it between parasite (A, B) and mutualist (C, D, F) groups. Conservation of iron metabolism genes across Wolbachia suggests iron homeostasis as a potential factor in its success. This study enhances our understanding of this globally pandemic endosymbiont, highlighting genetic patterns associated with host changes. Combined with future work on this strain, these genomic data could help provide potential new targets for plant-parasitic nematode control.
publisher	Nature Publishing Group
publishDate	2016
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5062116/
_version_	1613682211305816064

Genomic evidence for plant-parasitic nematodes as the earliest Wolbachia hosts

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