3D bioprinting matrices with controlled pore structure and release function guide in vitro self-organization of sweat gland
3D bioprinting matrices are novel platforms for tissue regeneration. Tissue self-organization is a critical process during regeneration that implies the features of organogenesis. However, it is not clear from the current evidences whether 3D printed construct plays a role in guiding tissue self-org...
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| Format: | Online |
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Nature Publishing Group
2016
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5046070/ |
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pubmed-50460702016-10-11 3D bioprinting matrices with controlled pore structure and release function guide in vitro self-organization of sweat gland Liu, Nanbo Huang, Sha Yao, Bin Xie, Jiangfan Wu, Xu Fu, Xiaobing Article 3D bioprinting matrices are novel platforms for tissue regeneration. Tissue self-organization is a critical process during regeneration that implies the features of organogenesis. However, it is not clear from the current evidences whether 3D printed construct plays a role in guiding tissue self-organization in vitro. Based on our previous study, we bioprinted a 3D matrix as the restrictive niche for direct sweat gland differentiation of epidermal progenitors by different pore structure (300-μm or 400-μm nozzle diameters printed) and reported a long-term gradual transition of differentiated cells into glandular morphogenesis occurs within the 3D construct in vitro. At the initial 14-day culture, an accelerated cell differentiation was achieved with inductive cues released along with gelatin reduction. After protein release completed, the 3D construct guide the self-organized formation of sweat gland tissues, which is similar to that of the natural developmental process. However, glandular morphogenesis was only observed in 300-μm–printed constructs. In the absence of 3D architectural support, glandular morphogenesis was not occurred. This striking finding made us to identify a previously unknown role of the 3D-printed structure in glandular tissue regeneration, and this self-organizing strategy can be applied to forming other tissues in vitro. Nature Publishing Group 2016-10-03 /pmc/articles/PMC5046070/ /pubmed/27694985 http://dx.doi.org/10.1038/srep34410 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Liu, Nanbo Huang, Sha Yao, Bin Xie, Jiangfan Wu, Xu Fu, Xiaobing |
| spellingShingle |
Liu, Nanbo Huang, Sha Yao, Bin Xie, Jiangfan Wu, Xu Fu, Xiaobing 3D bioprinting matrices with controlled pore structure and release function guide in vitro self-organization of sweat gland |
| author_facet |
Liu, Nanbo Huang, Sha Yao, Bin Xie, Jiangfan Wu, Xu Fu, Xiaobing |
| author_sort |
Liu, Nanbo |
| title |
3D bioprinting matrices with controlled pore structure and release function guide in vitro self-organization of sweat gland |
| title_short |
3D bioprinting matrices with controlled pore structure and release function guide in vitro self-organization of sweat gland |
| title_full |
3D bioprinting matrices with controlled pore structure and release function guide in vitro self-organization of sweat gland |
| title_fullStr |
3D bioprinting matrices with controlled pore structure and release function guide in vitro self-organization of sweat gland |
| title_full_unstemmed |
3D bioprinting matrices with controlled pore structure and release function guide in vitro self-organization of sweat gland |
| title_sort |
3d bioprinting matrices with controlled pore structure and release function guide in vitro self-organization of sweat gland |
| description |
3D bioprinting matrices are novel platforms for tissue regeneration. Tissue self-organization is a critical process during regeneration that implies the features of organogenesis. However, it is not clear from the current evidences whether 3D printed construct plays a role in guiding tissue self-organization in vitro. Based on our previous study, we bioprinted a 3D matrix as the restrictive niche for direct sweat gland differentiation of epidermal progenitors by different pore structure (300-μm or 400-μm nozzle diameters printed) and reported a long-term gradual transition of differentiated cells into glandular morphogenesis occurs within the 3D construct in vitro. At the initial 14-day culture, an accelerated cell differentiation was achieved with inductive cues released along with gelatin reduction. After protein release completed, the 3D construct guide the self-organized formation of sweat gland tissues, which is similar to that of the natural developmental process. However, glandular morphogenesis was only observed in 300-μm–printed constructs. In the absence of 3D architectural support, glandular morphogenesis was not occurred. This striking finding made us to identify a previously unknown role of the 3D-printed structure in glandular tissue regeneration, and this self-organizing strategy can be applied to forming other tissues in vitro. |
| publisher |
Nature Publishing Group |
| publishDate |
2016 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5046070/ |
| _version_ |
1613669276668919808 |