Knockdown of a laccase in Populus deltoides confers altered cell wall chemistry and increased sugar release

Plant laccases are thought to function in the oxidation of monolignols which leads to higher order lignin formation. Only a hand‐full of laccases in plants have been functionally evaluated, and as such little is known about the breadth of their impact on cell wall chemistry or structure. Here, we de...

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Main Authors:	Bryan, Anthony C., Jawdy, Sara, Gunter, Lee, Gjersing, Erica, Sykes, Robert, Hinchee, Maud A. W., Winkeler, Kimberly A., Collins, Cassandra M., Engle, Nancy, Tschaplinski, Timothy J., Yang, Xiaohan, Tuskan, Gerald A., Muchero, Wellington, Chen, Jin‐Gui
Format:	Online
Language:	English
Published:	John Wiley and Sons Inc. 2016
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5043505/

id	pubmed-5043505
recordtype	oai_dc
spelling	pubmed-50435052016-10-03 Knockdown of a laccase in Populus deltoides confers altered cell wall chemistry and increased sugar release Bryan, Anthony C. Jawdy, Sara Gunter, Lee Gjersing, Erica Sykes, Robert Hinchee, Maud A. W. Winkeler, Kimberly A. Collins, Cassandra M. Engle, Nancy Tschaplinski, Timothy J. Yang, Xiaohan Tuskan, Gerald A. Muchero, Wellington Chen, Jin‐Gui Research Articles Plant laccases are thought to function in the oxidation of monolignols which leads to higher order lignin formation. Only a hand‐full of laccases in plants have been functionally evaluated, and as such little is known about the breadth of their impact on cell wall chemistry or structure. Here, we describe a previously uncharacterized laccase from Populus, encoded by locus Potri.008G064000, whose reduced expression resulted in transgenic Populus trees with changes in syringyl/guaiacyl ratios as well as altered sugar release phenotypes. These phenotypes are consistent with plant biomass exhibiting reduced recalcitrance. Interestingly, the transgene effect on recalcitrance is dependent on a mild pretreatment prior to chemical extraction of sugars. Metabolite profiling suggests the transgene modulates phenolics that are associated with the cell wall structure. We propose that this particular laccase has a range of functions related to oxidation of phenolics and conjugation of flavonoids that interact with lignin in the cell wall. John Wiley and Sons Inc. 2016-04-15 2016-10 /pmc/articles/PMC5043505/ /pubmed/26997157 http://dx.doi.org/10.1111/pbi.12560 Text en © 2016 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Bryan, Anthony C. Jawdy, Sara Gunter, Lee Gjersing, Erica Sykes, Robert Hinchee, Maud A. W. Winkeler, Kimberly A. Collins, Cassandra M. Engle, Nancy Tschaplinski, Timothy J. Yang, Xiaohan Tuskan, Gerald A. Muchero, Wellington Chen, Jin‐Gui
spellingShingle	Bryan, Anthony C. Jawdy, Sara Gunter, Lee Gjersing, Erica Sykes, Robert Hinchee, Maud A. W. Winkeler, Kimberly A. Collins, Cassandra M. Engle, Nancy Tschaplinski, Timothy J. Yang, Xiaohan Tuskan, Gerald A. Muchero, Wellington Chen, Jin‐Gui Knockdown of a laccase in Populus deltoides confers altered cell wall chemistry and increased sugar release
author_facet	Bryan, Anthony C. Jawdy, Sara Gunter, Lee Gjersing, Erica Sykes, Robert Hinchee, Maud A. W. Winkeler, Kimberly A. Collins, Cassandra M. Engle, Nancy Tschaplinski, Timothy J. Yang, Xiaohan Tuskan, Gerald A. Muchero, Wellington Chen, Jin‐Gui
author_sort	Bryan, Anthony C.
title	Knockdown of a laccase in Populus deltoides confers altered cell wall chemistry and increased sugar release
title_short	Knockdown of a laccase in Populus deltoides confers altered cell wall chemistry and increased sugar release
title_full	Knockdown of a laccase in Populus deltoides confers altered cell wall chemistry and increased sugar release
title_fullStr	Knockdown of a laccase in Populus deltoides confers altered cell wall chemistry and increased sugar release
title_full_unstemmed	Knockdown of a laccase in Populus deltoides confers altered cell wall chemistry and increased sugar release
title_sort	knockdown of a laccase in populus deltoides confers altered cell wall chemistry and increased sugar release
description	Plant laccases are thought to function in the oxidation of monolignols which leads to higher order lignin formation. Only a hand‐full of laccases in plants have been functionally evaluated, and as such little is known about the breadth of their impact on cell wall chemistry or structure. Here, we describe a previously uncharacterized laccase from Populus, encoded by locus Potri.008G064000, whose reduced expression resulted in transgenic Populus trees with changes in syringyl/guaiacyl ratios as well as altered sugar release phenotypes. These phenotypes are consistent with plant biomass exhibiting reduced recalcitrance. Interestingly, the transgene effect on recalcitrance is dependent on a mild pretreatment prior to chemical extraction of sugars. Metabolite profiling suggests the transgene modulates phenolics that are associated with the cell wall structure. We propose that this particular laccase has a range of functions related to oxidation of phenolics and conjugation of flavonoids that interact with lignin in the cell wall.
publisher	John Wiley and Sons Inc.
publishDate	2016
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5043505/
_version_	1613666888586362880

Knockdown of a laccase in Populus deltoides confers altered cell wall chemistry and increased sugar release

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