Conjugation of biotin-coated luminescent quantum dots with single domain antibody-rhizavidin fusions

•Single domain antibody—rhizavidin fusion bioconjugated biotin coated quantum dots.•Provided facile and effective method to orient antibodies on QD surface.•Accomplished improved production of His-tagged rhizavidin (RZh) in E. coli.•Demonstrated utility of RZh as a replacement for tetrameric biotin...

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Main Authors:	Liu, Jinny L., Walper, Scott A., Turner, Kendrick B., Lee, Audrey Brozozog, Medintz, Igor L., Susumu, Kimihiro, Oh, Eunkeu, Zabetakis, Dan, Goldman, Ellen R., Anderson, George P.
Format:	Online
Language:	English
Published:	Elsevier 2016
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5040863/

id	pubmed-5040863
recordtype	oai_dc
spelling	pubmed-50408632017-03-28 Conjugation of biotin-coated luminescent quantum dots with single domain antibody-rhizavidin fusions Liu, Jinny L. Walper, Scott A. Turner, Kendrick B. Lee, Audrey Brozozog Medintz, Igor L. Susumu, Kimihiro Oh, Eunkeu Zabetakis, Dan Goldman, Ellen R. Anderson, George P. Article •Single domain antibody—rhizavidin fusion bioconjugated biotin coated quantum dots.•Provided facile and effective method to orient antibodies on QD surface.•Accomplished improved production of His-tagged rhizavidin (RZh) in E. coli.•Demonstrated utility of RZh as a replacement for tetrameric biotin binders. Elsevier 2016-03-03 /pmc/articles/PMC5040863/ /pubmed/28352525 http://dx.doi.org/10.1016/j.btre.2016.03.001 Text en http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Liu, Jinny L. Walper, Scott A. Turner, Kendrick B. Lee, Audrey Brozozog Medintz, Igor L. Susumu, Kimihiro Oh, Eunkeu Zabetakis, Dan Goldman, Ellen R. Anderson, George P.
spellingShingle	Liu, Jinny L. Walper, Scott A. Turner, Kendrick B. Lee, Audrey Brozozog Medintz, Igor L. Susumu, Kimihiro Oh, Eunkeu Zabetakis, Dan Goldman, Ellen R. Anderson, George P. Conjugation of biotin-coated luminescent quantum dots with single domain antibody-rhizavidin fusions
author_facet	Liu, Jinny L. Walper, Scott A. Turner, Kendrick B. Lee, Audrey Brozozog Medintz, Igor L. Susumu, Kimihiro Oh, Eunkeu Zabetakis, Dan Goldman, Ellen R. Anderson, George P.
author_sort	Liu, Jinny L.
title	Conjugation of biotin-coated luminescent quantum dots with single domain antibody-rhizavidin fusions
title_short	Conjugation of biotin-coated luminescent quantum dots with single domain antibody-rhizavidin fusions
title_full	Conjugation of biotin-coated luminescent quantum dots with single domain antibody-rhizavidin fusions
title_fullStr	Conjugation of biotin-coated luminescent quantum dots with single domain antibody-rhizavidin fusions
title_full_unstemmed	Conjugation of biotin-coated luminescent quantum dots with single domain antibody-rhizavidin fusions
title_sort	conjugation of biotin-coated luminescent quantum dots with single domain antibody-rhizavidin fusions
description	•Single domain antibody—rhizavidin fusion bioconjugated biotin coated quantum dots.•Provided facile and effective method to orient antibodies on QD surface.•Accomplished improved production of His-tagged rhizavidin (RZh) in E. coli.•Demonstrated utility of RZh as a replacement for tetrameric biotin binders.
publisher	Elsevier
publishDate	2016
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5040863/
_version_	1613664548088184832

Conjugation of biotin-coated luminescent quantum dots with single domain antibody-rhizavidin fusions

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