Muscle contraction is required to maintain the pool of muscle progenitors via YAP and NOTCH during fetal myogenesis

The importance of mechanical activity in the regulation of muscle progenitors during chick development has not been investigated. We show that immobilization decreases NOTCH activity and mimics a NOTCH loss-of-function phenotype, a reduction in the number of muscle progenitors and increased differen...

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Main Authors:	Esteves de Lima, Joana, Bonnin, Marie-Ange, Birchmeier, Carmen, Duprez, Delphine
Format:	Online
Language:	English
Published:	eLife Sciences Publications, Ltd 2016
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5030091/

id	pubmed-5030091
recordtype	oai_dc
spelling	pubmed-50300912016-09-21 Muscle contraction is required to maintain the pool of muscle progenitors via YAP and NOTCH during fetal myogenesis Esteves de Lima, Joana Bonnin, Marie-Ange Birchmeier, Carmen Duprez, Delphine Developmental Biology and Stem Cells The importance of mechanical activity in the regulation of muscle progenitors during chick development has not been investigated. We show that immobilization decreases NOTCH activity and mimics a NOTCH loss-of-function phenotype, a reduction in the number of muscle progenitors and increased differentiation. Ligand-induced NOTCH activation prevents the reduction of muscle progenitors and the increase of differentiation upon immobilization. Inhibition of NOTCH ligand activity in muscle fibers suffices to reduce the progenitor pool. Furthermore, immobilization reduces the activity of the transcriptional co-activator YAP and the expression of the NOTCH ligand JAG2 in muscle fibers. YAP forced-activity in muscle fibers prevents the decrease of JAG2 expression and the number of PAX7+ cells in immobilization conditions. Our results identify a novel mechanism acting downstream of muscle contraction, where YAP activates JAG2 expression in muscle fibers, which in turn regulates the pool of fetal muscle progenitors via NOTCH in a non-cell-autonomous manner. eLife Sciences Publications, Ltd 2016-08-24 /pmc/articles/PMC5030091/ /pubmed/27554485 http://dx.doi.org/10.7554/eLife.15593 Text en © 2016, Esteves de Lima et al http://creativecommons.org/licenses/by/4.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Esteves de Lima, Joana Bonnin, Marie-Ange Birchmeier, Carmen Duprez, Delphine
spellingShingle	Esteves de Lima, Joana Bonnin, Marie-Ange Birchmeier, Carmen Duprez, Delphine Muscle contraction is required to maintain the pool of muscle progenitors via YAP and NOTCH during fetal myogenesis
author_facet	Esteves de Lima, Joana Bonnin, Marie-Ange Birchmeier, Carmen Duprez, Delphine
author_sort	Esteves de Lima, Joana
title	Muscle contraction is required to maintain the pool of muscle progenitors via YAP and NOTCH during fetal myogenesis
title_short	Muscle contraction is required to maintain the pool of muscle progenitors via YAP and NOTCH during fetal myogenesis
title_full	Muscle contraction is required to maintain the pool of muscle progenitors via YAP and NOTCH during fetal myogenesis
title_fullStr	Muscle contraction is required to maintain the pool of muscle progenitors via YAP and NOTCH during fetal myogenesis
title_full_unstemmed	Muscle contraction is required to maintain the pool of muscle progenitors via YAP and NOTCH during fetal myogenesis
title_sort	muscle contraction is required to maintain the pool of muscle progenitors via yap and notch during fetal myogenesis
description	The importance of mechanical activity in the regulation of muscle progenitors during chick development has not been investigated. We show that immobilization decreases NOTCH activity and mimics a NOTCH loss-of-function phenotype, a reduction in the number of muscle progenitors and increased differentiation. Ligand-induced NOTCH activation prevents the reduction of muscle progenitors and the increase of differentiation upon immobilization. Inhibition of NOTCH ligand activity in muscle fibers suffices to reduce the progenitor pool. Furthermore, immobilization reduces the activity of the transcriptional co-activator YAP and the expression of the NOTCH ligand JAG2 in muscle fibers. YAP forced-activity in muscle fibers prevents the decrease of JAG2 expression and the number of PAX7+ cells in immobilization conditions. Our results identify a novel mechanism acting downstream of muscle contraction, where YAP activates JAG2 expression in muscle fibers, which in turn regulates the pool of fetal muscle progenitors via NOTCH in a non-cell-autonomous manner.
publisher	eLife Sciences Publications, Ltd
publishDate	2016
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5030091/
_version_	1613656318621515776

Muscle contraction is required to maintain the pool of muscle progenitors via YAP and NOTCH during fetal myogenesis

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