In vivo induction of neutrophil chemotaxis by secretory aspartyl proteinases of Candida albicans

Secretory aspartyl proteinases (Saps) of Candida albicans are key virulence traits which cause inflammasome-dependent, aseptic inflammation in a mouse model of vaginitis. In this paper, neutrophil migration in response to Sap2, Sap6 and chemo-attractive products released from Sap-treated vaginal epi...

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Main Authors:	Gabrielli, Elena, Sabbatini, Samuele, Roselletti, Elena, Kasper, Lydia, Perito, Stefano, Hube, Bernhard, Cassone, Antonio, Vecchiarelli, Anna, Pericolini, Eva
Format:	Online
Language:	English
Published:	Taylor & Francis 2016
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5029300/

id	pubmed-5029300
recordtype	oai_dc
spelling	pubmed-50293002016-09-30 In vivo induction of neutrophil chemotaxis by secretory aspartyl proteinases of Candida albicans Gabrielli, Elena Sabbatini, Samuele Roselletti, Elena Kasper, Lydia Perito, Stefano Hube, Bernhard Cassone, Antonio Vecchiarelli, Anna Pericolini, Eva Brief Report Secretory aspartyl proteinases (Saps) of Candida albicans are key virulence traits which cause inflammasome-dependent, aseptic inflammation in a mouse model of vaginitis. In this paper, neutrophil migration in response to Sap2, Sap6 and chemo-attractive products released from Sap-treated vaginal epithelium was measured in vitro, ex vivo and in vivo. Our results show that Sap2 and Sap6 induce neutrophil migration and production of potent chemoattractive chemokines such as IL-8 and MIP-2 by vaginal epithelial cells. Our data suggest that at least part of MIP-2 production depends upon IL-1β activity. The vaginal fluid of Candida-infected mice contained a heat-labile inhibitor of neutrophil candidacidal activity that was absent from the vaginal fluid of Sap-treated mice. Overall, our data provide additional information on the capacity of C. albicans Saps to cause aseptic vaginal inflammation and highlight the potential role of some chemokines released from vaginal epithelial cells in this phenomenon. Taylor & Francis 2016-04-29 /pmc/articles/PMC5029300/ /pubmed/27127904 http://dx.doi.org/10.1080/21505594.2016.1184385 Text en © 2016 The Author(s). Published with license by Taylor & Francis. http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Gabrielli, Elena Sabbatini, Samuele Roselletti, Elena Kasper, Lydia Perito, Stefano Hube, Bernhard Cassone, Antonio Vecchiarelli, Anna Pericolini, Eva
spellingShingle	Gabrielli, Elena Sabbatini, Samuele Roselletti, Elena Kasper, Lydia Perito, Stefano Hube, Bernhard Cassone, Antonio Vecchiarelli, Anna Pericolini, Eva In vivo induction of neutrophil chemotaxis by secretory aspartyl proteinases of Candida albicans
author_facet	Gabrielli, Elena Sabbatini, Samuele Roselletti, Elena Kasper, Lydia Perito, Stefano Hube, Bernhard Cassone, Antonio Vecchiarelli, Anna Pericolini, Eva
author_sort	Gabrielli, Elena
title	In vivo induction of neutrophil chemotaxis by secretory aspartyl proteinases of Candida albicans
title_short	In vivo induction of neutrophil chemotaxis by secretory aspartyl proteinases of Candida albicans
title_full	In vivo induction of neutrophil chemotaxis by secretory aspartyl proteinases of Candida albicans
title_fullStr	In vivo induction of neutrophil chemotaxis by secretory aspartyl proteinases of Candida albicans
title_full_unstemmed	In vivo induction of neutrophil chemotaxis by secretory aspartyl proteinases of Candida albicans
title_sort	in vivo induction of neutrophil chemotaxis by secretory aspartyl proteinases of candida albicans
description	Secretory aspartyl proteinases (Saps) of Candida albicans are key virulence traits which cause inflammasome-dependent, aseptic inflammation in a mouse model of vaginitis. In this paper, neutrophil migration in response to Sap2, Sap6 and chemo-attractive products released from Sap-treated vaginal epithelium was measured in vitro, ex vivo and in vivo. Our results show that Sap2 and Sap6 induce neutrophil migration and production of potent chemoattractive chemokines such as IL-8 and MIP-2 by vaginal epithelial cells. Our data suggest that at least part of MIP-2 production depends upon IL-1β activity. The vaginal fluid of Candida-infected mice contained a heat-labile inhibitor of neutrophil candidacidal activity that was absent from the vaginal fluid of Sap-treated mice. Overall, our data provide additional information on the capacity of C. albicans Saps to cause aseptic vaginal inflammation and highlight the potential role of some chemokines released from vaginal epithelial cells in this phenomenon.
publisher	Taylor & Francis
publishDate	2016
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5029300/
_version_	1613655719960117248

In vivo induction of neutrophil chemotaxis by secretory aspartyl proteinases of Candida albicans

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