Exploring the transcriptome of Staphylococcus aureus in its natural niche

Staphylococcus aureus is an important human pathogen and commensal, where the human nose is the predominant reservoir. To better understand its behavior in this environmental niche, RNA was extracted from the anterior nares of three documented S. aureus carriers and the metatranscriptome analyzed by...

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Main Authors:	Chaves-Moreno, Diego, Wos-Oxley, Melissa L., Jáuregui, Ruy, Medina, Eva, Oxley, Andrew PA, Pieper, Dietmar H.
Format:	Online
Language:	English
Published:	Nature Publishing Group 2016
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5027550/

id	pubmed-5027550
recordtype	oai_dc
spelling	pubmed-50275502016-09-22 Exploring the transcriptome of Staphylococcus aureus in its natural niche Chaves-Moreno, Diego Wos-Oxley, Melissa L. Jáuregui, Ruy Medina, Eva Oxley, Andrew PA Pieper, Dietmar H. Article Staphylococcus aureus is an important human pathogen and commensal, where the human nose is the predominant reservoir. To better understand its behavior in this environmental niche, RNA was extracted from the anterior nares of three documented S. aureus carriers and the metatranscriptome analyzed by RNAseq. In addition, the in vivo transcriptomes were compared to previously published transcriptomes of two in vitro grown S. aureus strains. None of the in vitro conditions, even growth in medium resembling the anterior nares environment, mimicked in vivo conditions. Survival in the nose was strongly controlled by the limitation of iron and evident by the expression of iron acquisition systems. S. aureus populations in different individuals clearly experience different environmental stresses, which they attempt to overcome by the expression of compatible solute biosynthetic pathways, changes in their cell wall composition and synthesis of general stress proteins. Moreover, the expression of adhesins was also important for colonization of the anterior nares. However, different S. aureus strains also showed different in vivo behavior. The assessment of general in vivo expression patterns and commonalities between different S. aureus strains will in the future result in new knowledge based strategies for controlling colonization. Nature Publishing Group 2016-09-19 /pmc/articles/PMC5027550/ /pubmed/27641137 http://dx.doi.org/10.1038/srep33174 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Chaves-Moreno, Diego Wos-Oxley, Melissa L. Jáuregui, Ruy Medina, Eva Oxley, Andrew PA Pieper, Dietmar H.
spellingShingle	Chaves-Moreno, Diego Wos-Oxley, Melissa L. Jáuregui, Ruy Medina, Eva Oxley, Andrew PA Pieper, Dietmar H. Exploring the transcriptome of Staphylococcus aureus in its natural niche
author_facet	Chaves-Moreno, Diego Wos-Oxley, Melissa L. Jáuregui, Ruy Medina, Eva Oxley, Andrew PA Pieper, Dietmar H.
author_sort	Chaves-Moreno, Diego
title	Exploring the transcriptome of Staphylococcus aureus in its natural niche
title_short	Exploring the transcriptome of Staphylococcus aureus in its natural niche
title_full	Exploring the transcriptome of Staphylococcus aureus in its natural niche
title_fullStr	Exploring the transcriptome of Staphylococcus aureus in its natural niche
title_full_unstemmed	Exploring the transcriptome of Staphylococcus aureus in its natural niche
title_sort	exploring the transcriptome of staphylococcus aureus in its natural niche
description	Staphylococcus aureus is an important human pathogen and commensal, where the human nose is the predominant reservoir. To better understand its behavior in this environmental niche, RNA was extracted from the anterior nares of three documented S. aureus carriers and the metatranscriptome analyzed by RNAseq. In addition, the in vivo transcriptomes were compared to previously published transcriptomes of two in vitro grown S. aureus strains. None of the in vitro conditions, even growth in medium resembling the anterior nares environment, mimicked in vivo conditions. Survival in the nose was strongly controlled by the limitation of iron and evident by the expression of iron acquisition systems. S. aureus populations in different individuals clearly experience different environmental stresses, which they attempt to overcome by the expression of compatible solute biosynthetic pathways, changes in their cell wall composition and synthesis of general stress proteins. Moreover, the expression of adhesins was also important for colonization of the anterior nares. However, different S. aureus strains also showed different in vivo behavior. The assessment of general in vivo expression patterns and commonalities between different S. aureus strains will in the future result in new knowledge based strategies for controlling colonization.
publisher	Nature Publishing Group
publishDate	2016
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5027550/
_version_	1613654609651302400

Exploring the transcriptome of Staphylococcus aureus in its natural niche

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