The Multivesicular Bodies (MVBs)-Localized AAA ATPase LRD6-6 Inhibits Immunity and Cell Death Likely through Regulating MVBs-Mediated Vesicular Trafficking in Rice

The Multivesicular Bodies (MVBs)-Localized AAA ATPase LRD6-6 Inhibits Immunity and Cell Death Likely through Regulating MVBs-Mediated Vesicular Trafficking in Rice

Previous studies have shown that multivesicular bodies (MVBs)/endosomes-mediated vesicular trafficking may play key roles in plant immunity and cell death. However, the molecular regulation is poorly understood in rice. Here we report the identification and characterization of a MVBs-localized AAA A...

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Main Authors: Zhu, Xiaobo, Yin, Junjie, Liang, Sihui, Liang, Ruihong, Zhou, Xiaogang, Chen, Zhixiong, Zhao, Wen, Wang, Jing, Li, Weitao, He, Min, Yuan, Can, Miyamoto, Koji, Ma, Bingtian, Wang, Jichun, Qin, Peng, Chen, Weilan, Wang, Yuping, Wang, Wenming, Wu, Xianjun, Yamane, Hisakazu, Zhu, Lihuang, Li, Shigui, Chen, Xuewei
Format: Online
Language:English
Published: Public Library of Science 2016
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5019419/
id pubmed-5019419
recordtype oai_dc
spelling pubmed-50194192016-09-27 The Multivesicular Bodies (MVBs)-Localized AAA ATPase LRD6-6 Inhibits Immunity and Cell Death Likely through Regulating MVBs-Mediated Vesicular Trafficking in Rice Zhu, Xiaobo Yin, Junjie Liang, Sihui Liang, Ruihong Zhou, Xiaogang Chen, Zhixiong Zhao, Wen Wang, Jing Li, Weitao He, Min Yuan, Can Miyamoto, Koji Ma, Bingtian Wang, Jichun Qin, Peng Chen, Weilan Wang, Yuping Wang, Wenming Wu, Xianjun Yamane, Hisakazu Zhu, Lihuang Li, Shigui Chen, Xuewei Research Article Previous studies have shown that multivesicular bodies (MVBs)/endosomes-mediated vesicular trafficking may play key roles in plant immunity and cell death. However, the molecular regulation is poorly understood in rice. Here we report the identification and characterization of a MVBs-localized AAA ATPase LRD6-6 in rice. Disruption of LRD6-6 leads to enhanced immunity and cell death in rice. The ATPase activity and homo-dimerization of LRD6-6 is essential for its regulation on plant immunity and cell death. An ATPase inactive mutation (LRD6-6E315Q) leads to dominant-negative inhibition in plants. The LRD6-6 protein co-localizes with the MVBs marker protein RabF1/ARA6 and interacts with ESCRT-III components OsSNF7 and OsVPS2. Further analysis reveals that LRD6-6 is required for MVBs-mediated vesicular trafficking and inhibits the biosynthesis of antimicrobial compounds. Collectively, our study shows that the AAA ATPase LRD6-6 inhibits plant immunity and cell death most likely through modulating MVBs-mediated vesicular trafficking in rice. Public Library of Science 2016-09-12 /pmc/articles/PMC5019419/ /pubmed/27618555 http://dx.doi.org/10.1371/journal.pgen.1006311 Text en © 2016 Zhu et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author Zhu, Xiaobo
Yin, Junjie
Liang, Sihui
Liang, Ruihong
Zhou, Xiaogang
Chen, Zhixiong
Zhao, Wen
Wang, Jing
Li, Weitao
He, Min
Yuan, Can
Miyamoto, Koji
Ma, Bingtian
Wang, Jichun
Qin, Peng
Chen, Weilan
Wang, Yuping
Wang, Wenming
Wu, Xianjun
Yamane, Hisakazu
Zhu, Lihuang
Li, Shigui
Chen, Xuewei
spellingShingle Zhu, Xiaobo
Yin, Junjie
Liang, Sihui
Liang, Ruihong
Zhou, Xiaogang
Chen, Zhixiong
Zhao, Wen
Wang, Jing
Li, Weitao
He, Min
Yuan, Can
Miyamoto, Koji
Ma, Bingtian
Wang, Jichun
Qin, Peng
Chen, Weilan
Wang, Yuping
Wang, Wenming
Wu, Xianjun
Yamane, Hisakazu
Zhu, Lihuang
Li, Shigui
Chen, Xuewei
The Multivesicular Bodies (MVBs)-Localized AAA ATPase LRD6-6 Inhibits Immunity and Cell Death Likely through Regulating MVBs-Mediated Vesicular Trafficking in Rice
author_facet Zhu, Xiaobo
Yin, Junjie
Liang, Sihui
Liang, Ruihong
Zhou, Xiaogang
Chen, Zhixiong
Zhao, Wen
Wang, Jing
Li, Weitao
He, Min
Yuan, Can
Miyamoto, Koji
Ma, Bingtian
Wang, Jichun
Qin, Peng
Chen, Weilan
Wang, Yuping
Wang, Wenming
Wu, Xianjun
Yamane, Hisakazu
Zhu, Lihuang
Li, Shigui
Chen, Xuewei
author_sort Zhu, Xiaobo
title The Multivesicular Bodies (MVBs)-Localized AAA ATPase LRD6-6 Inhibits Immunity and Cell Death Likely through Regulating MVBs-Mediated Vesicular Trafficking in Rice
title_short The Multivesicular Bodies (MVBs)-Localized AAA ATPase LRD6-6 Inhibits Immunity and Cell Death Likely through Regulating MVBs-Mediated Vesicular Trafficking in Rice
title_full The Multivesicular Bodies (MVBs)-Localized AAA ATPase LRD6-6 Inhibits Immunity and Cell Death Likely through Regulating MVBs-Mediated Vesicular Trafficking in Rice
title_fullStr The Multivesicular Bodies (MVBs)-Localized AAA ATPase LRD6-6 Inhibits Immunity and Cell Death Likely through Regulating MVBs-Mediated Vesicular Trafficking in Rice
title_full_unstemmed The Multivesicular Bodies (MVBs)-Localized AAA ATPase LRD6-6 Inhibits Immunity and Cell Death Likely through Regulating MVBs-Mediated Vesicular Trafficking in Rice
title_sort multivesicular bodies (mvbs)-localized aaa atpase lrd6-6 inhibits immunity and cell death likely through regulating mvbs-mediated vesicular trafficking in rice
description Previous studies have shown that multivesicular bodies (MVBs)/endosomes-mediated vesicular trafficking may play key roles in plant immunity and cell death. However, the molecular regulation is poorly understood in rice. Here we report the identification and characterization of a MVBs-localized AAA ATPase LRD6-6 in rice. Disruption of LRD6-6 leads to enhanced immunity and cell death in rice. The ATPase activity and homo-dimerization of LRD6-6 is essential for its regulation on plant immunity and cell death. An ATPase inactive mutation (LRD6-6E315Q) leads to dominant-negative inhibition in plants. The LRD6-6 protein co-localizes with the MVBs marker protein RabF1/ARA6 and interacts with ESCRT-III components OsSNF7 and OsVPS2. Further analysis reveals that LRD6-6 is required for MVBs-mediated vesicular trafficking and inhibits the biosynthesis of antimicrobial compounds. Collectively, our study shows that the AAA ATPase LRD6-6 inhibits plant immunity and cell death most likely through modulating MVBs-mediated vesicular trafficking in rice.
publisher Public Library of Science
publishDate 2016
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5019419/
_version_ 1613648997485903872

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