Site-specific fluorescent labeling to visualize membrane translocation of a myristoyl switch protein
Fluorescence approaches have been widely used for elucidating the dynamics of protein-membrane interactions in cells and model systems. However, non-specific multi-site fluorescent labeling often results in a loss of native structure and function, and single cysteine labeling is not feasible when na...
Main Authors: | Yang, Sung-Tae, Lim, Sung In, Kiessling, Volker, Kwon, Inchan, Tamm, Lukas K. |
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Format: | Online |
Language: | English |
Published: |
Nature Publishing Group
2016
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Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5015116/ |
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