Kidney Expression of Toll Like Receptors in Lupus Nephritis: Quantification and Clinicopathological Correlations

Objective. The study aimed at locating and quantifying Toll Like Receptor (TLR) 3, 7, 8, and 9 expression in kidney of patients with lupus nephritis (LN) and correlating them with clinicopathological features. Methods. Kidney sections from 26 LN patients and 4 controls were analyzed by immunohistoc...

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Main Authors:	Conti, Fabrizio, Spinelli, Francesca Romana, Truglia, Simona, Miranda, Francesca, Alessandri, Cristiano, Ceccarelli, Fulvia, Bombardieri, Michele, Giannakakis, Konstantinos, Valesini, Guido
Format:	Online
Language:	English
Published:	Hindawi Publishing Corporation 2016
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5011205/

id	pubmed-5011205
recordtype	oai_dc
spelling	pubmed-50112052016-09-15 Kidney Expression of Toll Like Receptors in Lupus Nephritis: Quantification and Clinicopathological Correlations Conti, Fabrizio Spinelli, Francesca Romana Truglia, Simona Miranda, Francesca Alessandri, Cristiano Ceccarelli, Fulvia Bombardieri, Michele Giannakakis, Konstantinos Valesini, Guido Research Article Objective. The study aimed at locating and quantifying Toll Like Receptor (TLR) 3, 7, 8, and 9 expression in kidney of patients with lupus nephritis (LN) and correlating them with clinicopathological features. Methods. Kidney sections from 26 LN patients and 4 controls were analyzed by immunohistochemistry using anti-human TLR3, TLR7, TLR8, and TLR9 polyclonal antibodies; the number of TLR-positive nuclei/mm2 was evaluated on digitalized images. Results. Compared to controls, LN showed a significantly higher amount of glomerular and tubulointerstitial TLR9 (p = 0.003 and p = 0.007), whole and tubulointerstitial TLR3 (p = 0.026 and p = 0.031), and a higher tubulointerstitial TLR7 (p = 0.022). TLR9 positively correlated with activity index (p = 0.0063) and tubular TLR7 with chronicity index (p = 0.026). TLR9 positively correlated with Renal-SLEDAI (p = 0.01). Conclusions. This is the first study quantifying kidney expressions of TLRs in LN patients; the results show an overexpression of TLR3, TLR7, and TLR9 and demonstrate a correlation with clinicopathological indices supporting a role of these mediators in the pathogenesis of LN. Hindawi Publishing Corporation 2016 2016-08-21 /pmc/articles/PMC5011205/ /pubmed/27635115 http://dx.doi.org/10.1155/2016/7697592 Text en Copyright © 2016 Fabrizio Conti et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Conti, Fabrizio Spinelli, Francesca Romana Truglia, Simona Miranda, Francesca Alessandri, Cristiano Ceccarelli, Fulvia Bombardieri, Michele Giannakakis, Konstantinos Valesini, Guido
spellingShingle	Conti, Fabrizio Spinelli, Francesca Romana Truglia, Simona Miranda, Francesca Alessandri, Cristiano Ceccarelli, Fulvia Bombardieri, Michele Giannakakis, Konstantinos Valesini, Guido Kidney Expression of Toll Like Receptors in Lupus Nephritis: Quantification and Clinicopathological Correlations
author_facet	Conti, Fabrizio Spinelli, Francesca Romana Truglia, Simona Miranda, Francesca Alessandri, Cristiano Ceccarelli, Fulvia Bombardieri, Michele Giannakakis, Konstantinos Valesini, Guido
author_sort	Conti, Fabrizio
title	Kidney Expression of Toll Like Receptors in Lupus Nephritis: Quantification and Clinicopathological Correlations
title_short	Kidney Expression of Toll Like Receptors in Lupus Nephritis: Quantification and Clinicopathological Correlations
title_full	Kidney Expression of Toll Like Receptors in Lupus Nephritis: Quantification and Clinicopathological Correlations
title_fullStr	Kidney Expression of Toll Like Receptors in Lupus Nephritis: Quantification and Clinicopathological Correlations
title_full_unstemmed	Kidney Expression of Toll Like Receptors in Lupus Nephritis: Quantification and Clinicopathological Correlations
title_sort	kidney expression of toll like receptors in lupus nephritis: quantification and clinicopathological correlations
description	Objective. The study aimed at locating and quantifying Toll Like Receptor (TLR) 3, 7, 8, and 9 expression in kidney of patients with lupus nephritis (LN) and correlating them with clinicopathological features. Methods. Kidney sections from 26 LN patients and 4 controls were analyzed by immunohistochemistry using anti-human TLR3, TLR7, TLR8, and TLR9 polyclonal antibodies; the number of TLR-positive nuclei/mm2 was evaluated on digitalized images. Results. Compared to controls, LN showed a significantly higher amount of glomerular and tubulointerstitial TLR9 (p = 0.003 and p = 0.007), whole and tubulointerstitial TLR3 (p = 0.026 and p = 0.031), and a higher tubulointerstitial TLR7 (p = 0.022). TLR9 positively correlated with activity index (p = 0.0063) and tubular TLR7 with chronicity index (p = 0.026). TLR9 positively correlated with Renal-SLEDAI (p = 0.01). Conclusions. This is the first study quantifying kidney expressions of TLRs in LN patients; the results show an overexpression of TLR3, TLR7, and TLR9 and demonstrate a correlation with clinicopathological indices supporting a role of these mediators in the pathogenesis of LN.
publisher	Hindawi Publishing Corporation
publishDate	2016
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5011205/
_version_	1613643230086168576

Kidney Expression of Toll Like Receptors in Lupus Nephritis: Quantification and Clinicopathological Correlations

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